Knee Surgery, Sports Traumatology, Arthroscopy

, Volume 15, Issue 9, pp 1083–1093 | Cite as

The role of the synovium in repairing cartilage defects

  • Ayato Miyamoto
  • Masataka Deie
  • Takuma Yamasaki
  • Atsuo Nakamae
  • Rikuo Shinomiya
  • Nobuo Adachi
  • Mitsuo Ochi
Knee
First Online:
Received:
Accepted:

Abstract

The purpose of this study was to examine the role of the synovium in the transitional zone between the articular cartilage and the synovial membrane in cartilage repair and the relationship between the origin of the repaired cartilage and the grafted synovium. We used 8-week-old Sprague Dawley (SD) rats and green fluorescent protein (GFP) transgenic rats. In study 1, a full-thickness cartilage defect was created at the medial condyle of the femur, and the synovium 5 × 5 mm extending up to the cartilage defect was resected in the left knee (cartilage defect without synovium group) but not resected in the right knee (cartilage defect with intact synovium group). In study 2, after the creation of a full-thickness cartilage defect and resection of the synovium, the synovium of the GFP rats was transplanted into the unilateral knee (cartilage defect with transpl.synovium group). At 2, 4, 6, and 8 weeks after surgery, we evaluated the repaired tissue in cartilage defects histologically and immunohistochemically, and the expression of aggrecan and type II collagen in the repaired tissue was also investigated using reverse transcriptase-polymerase chain reactions (RT-PCR). At 6 and 8 weeks after surgery, the defect was filled with cartilage-like tissue in cartilage defect with intact synovium group and cartilage defect with transpl.synovium group, but not in cartilage defect without synovium group. GFP positive cells were observed in the repaired tissue and the expression of aggrecan and type II collagen was found in cartilage defect with transpl.synovium group. We concluded that the synovium in the transitional zone between the articular cartilage and the grafted synovial membrane invades the cartilage defects where the cells could be detected as GFP-positive cells. Those cells may take part in the repair and may induce chondrogenesis.

Keywords

Synovium Transitional zone Chondrogenesis Cartilage defect Cartilage repair 
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References

  1. 1.
    Allard SA, Bayliss MT, Maini RN (1990) The synovium-cartilage junction of the normal human knee. Arthritis Rheum 33:1170–1179PubMedCrossRefGoogle Scholar
  2. 2.
    Allard SA, Muirden KD, Maini RN (1991) Correlation of histopathological features of pannus with patterns of damage in different joints in rheumatoid arthritis. Ann Rheum Dis 50:278–283PubMedGoogle Scholar
  3. 3.
    Arai K, Hotokebucki T, Miyahara H, Arita C, Mohtai M, Sugioka Y, Kaibara N (1989) Limb allografts in rats immunosuppressed with FK-506. Transplantation 48:782–786PubMedCrossRefGoogle Scholar
  4. 4.
    Chu CQ, Field M, Abney E, Zheng RQH, Allard S, Feldmann M, Maini RN (1991) Transforming growth factor-β1 in rheumatoid synovial membrane and cartilage/pannus junction. Clin Exp Immuno 86:380–386CrossRefGoogle Scholar
  5. 5.
    Cosimo De Bari, Francesco Dell’Accio, Przemyslaw Tyzanowski, Frank P Luyten (2001) Multipotent mesenchymal stem cells from adult human synovial membrane. Arthritis Rheum 44:1928–1942CrossRefGoogle Scholar
  6. 6.
    Fickert S, Fildler J, Brenner RE (2003) Identification, quantification and isolation of mesenchymal progenitor cells from osteoarthritic synovium by fluorescence automated cell sorting. Osteoarthritis Cartilage 11:790–800PubMedCrossRefGoogle Scholar
  7. 7.
    Hunziker EB, Rosenberg LC (1996) Repair of partial-thickness defects in articular cartilage: cell recruitment from the synovial membrane. J Bone Joint Surg Am 78-A:721–733Google Scholar
  8. 8.
    Iwata H, Ono S, Sato K, Sato T, Kawamura M (1993) Bone morphobenitic protein-induced muscle- and synovium-derived cartilage differentiation in vitro. Clin Orthop 296:295–300PubMedGoogle Scholar
  9. 9.
    Min Z, Jones NF (1995) Limb transplantation in rats: immunosuppression with FK-506. J Hand Surg Am 20(1):77–87PubMedCrossRefGoogle Scholar
  10. 10.
    Nishimura K, Solchaga LA, Caplan AI, Yoo JU, Goldberg VM, Johnstone B (1999) Chondroprogenitor cells of synovial tissue. Arthritis Rheum 42:2631–2637PubMedCrossRefGoogle Scholar
  11. 11.
    Okabe M, Ikawa M, Kominami K, Nakanishi T, Nishimune Y (1997) ‘Green mice’as a source of ubiquitous green cells. FEBS Lett 407:313–319PubMedCrossRefGoogle Scholar
  12. 12.
    Palmer DG, Selvendran Y, Allen C, Revell PA, Hogg N (1985) Features of synovial membrane identified with monoclonal antibodies. Clin Exp Immunol 59:529–538PubMedGoogle Scholar
  13. 13.
    Panasyuk A, Colantuoni G, Khatib AM, Lomri A, Mitrovic DR (2003) Human synovium produces substances that inhibit DNA and stimulate proteoglycan and collagen synthesis by cultured human articular chondrocytes and synovial fibroblasts. Scand J Rheumatol 32:240–246PubMedCrossRefGoogle Scholar
  14. 14.
    Pineda S, Pollack A, Stevenson S, Goldberg V, Caplan A (1992) A semiquantitative scale for histologic grading of articular cartilage repair. Acta Anat 143:335–340PubMedCrossRefGoogle Scholar
  15. 15.
    Rothwell AG (1990) Synovium transplantation onto the cartilage denuded patellar groove of the sheep knee joint. Orthopedics 13(4):434–442Google Scholar
  16. 16.
    Shibakawa A, Aoki H, Masuko-Hongo K, Kato T, Tanaka M, Nishioka K, Nakamura H (2003) Presence of pannus-like tissue on osteoarthritic cartilage and its histological character. Osteoarthritis Cartilage 11:133–140PubMedCrossRefGoogle Scholar
  17. 17.
    Shiozawa S, Shiozawa K, Fujita T (1983) Morphologic observations in the early phase of the cartilage-pannus junction. Arthritis Rheum 26:472–478PubMedCrossRefGoogle Scholar
  18. 18.
    Thompson AM, Stockwell RA (1983) An ultrastructural study of the marginal transitional zone in the rabbit knee joint. J Anat 136:701–713PubMedGoogle Scholar
  19. 19.
    Vidinov N, Djerov A (2002) Ultrastructural changes in the transitional zone between articular cartilage and synovial membrane during the development of experimental osteoarthrosis. Int Orthop 26:137–140PubMedCrossRefGoogle Scholar
  20. 20.
    Yuan GH, Tanaka M, Masuko-Hongo K, Shibakawa A, Kato T, Nishioka K, Nakamura H (2004) Characterization of cells from pannus-like tissue over articular cartilage of advanced osteoarthritis. Osteoarthritis Cartilage 12:38–45PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Ayato Miyamoto
    • 1
  • Masataka Deie
    • 1
  • Takuma Yamasaki
    • 1
  • Atsuo Nakamae
    • 1
  • Rikuo Shinomiya
    • 1
  • Nobuo Adachi
    • 1
  • Mitsuo Ochi
    • 1
  1. 1.Department of Orthopaedic Surgery, Graduate School of Biomedical SciencesHiroshima UniversityHiroshimaJapan

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