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Intensive Care Medicine

, Volume 24, Issue 9, pp 973–976 | Cite as

Thiopental inhibits migration of human leukocytes through human endothelial cell monolayers in vitro

  • R. HofbauerEmail author
  • H. Salfinger
  • S. Kapiotis
  • D. Moser
  • M. Frass
Experimental

Abstract

Objectives

The interactions between blood and vascular wall cells are essential for the understanding of pathophysiologic processes, e. g. inflammation. The influence of the anesthetic drug thiopental on leukocyte function is well documented. Recently, an inhibitory effect of thiopental on leukocyte chemotaxis in a Boyden chamber assay (i. e. endothelial cells were not included) was demonstrated. In vivo, leukocytes have to interact with endothelial cell monolayers to invade the tissue. The influence of thiopental on a monolayer of endothelial cells has not yet been investigated. The aim of the current study was to investigate the influence of thiopental on the migration of leukocytes through endothelial cell monolayers (ECM).

Material and methods

Human umbilical vein endothelial cells (HUVEC) were isolated and cultured on microporous membrane filters to achieve a monolayer. Isolated polymorphonuclear leukocytes (PMNL) as well as ECM were preincubated with different concentrations of thiopental. The rate of leukocyte migration against the chemotactic protein formyl-methyl-leucyl-phenyla-lanine was measured (n=7). Thiopental was able to reduce the amount of leukocyte migration through ECM significantly.

Conclusion

In conclusion, we could show that thiopental is able to reduce the migration of PMNL through ECM significantly.

Key words

Thiopental Endothelial cells Neutrophils Migration In vitro assay PMNL 

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Copyright information

© Springer-Verlag 1998

Authors and Affiliations

  • R. Hofbauer
    • 1
    • 2
    Email author
  • H. Salfinger
    • 1
  • S. Kapiotis
    • 2
  • D. Moser
    • 3
  • M. Frass
    • 4
  1. 1.Department of Anesthesiology and Intensive Care MedicineUniversity of ViennaViennaAustria
  2. 2.Clinical Institute of Medical and Chemical Laboratory DiagnosticsUniversity of ViennaViennaAustria
  3. 3.Department of Oral and Maxillofacial SurgeryUniversity of ViennaViennaAustria
  4. 4.Department of Internal Medicine I, Medical Intensive Care Unit 131.2University of ViennaViennaAustria

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