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Intensive Care Medicine

, Volume 44, Issue 8, pp 1203–1211 | Cite as

Pathogen colonization of the gastrointestinal microbiome at intensive care unit admission and risk for subsequent death or infection

  • Daniel E. Freedberg
  • Margaret J. Zhou
  • Margot E. Cohen
  • Medini K. Annavajhala
  • Sabrina Khan
  • Dagmara I. Moscoso
  • Christian Brooks
  • Susan Whittier
  • David H. Chong
  • Anne-Catrin Uhlemann
  • Julian A. Abrams
Original

Abstract

Purpose

Loss of colonization resistance within the gastrointestinal microbiome facilitates the expansion of pathogens and has been associated with death and infection in select populations. We tested whether gut microbiome features at the time of intensive care unit (ICU) admission predict death or infection.

Methods

This was a prospective cohort study of medical ICU adults. Rectal surveillance swabs were performed at admission, selectively cultured for vancomycin-resistant Enterococcus (VRE), and assessed using 16S rRNA gene sequencing. Patients were followed for 30 days for death or culture-proven bacterial infection.

Results

Of 301 patients, 123 (41%) developed culture-proven infections and 76 (25%) died. Fecal biodiversity (Shannon index) did not differ based on death or infection (p = 0.49). The presence of specific pathogens at ICU admission was associated with subsequent infection with the same organism for Escherichia coli, Pseudomonas spp., Klebsiella spp., and Clostridium difficile, and VRE at admission was associated with subsequent Enterococcus infection. In a multivariable model adjusting for severity of illness, VRE colonization and Enterococcus domination (≥ 30% 16S reads) were both associated with death or all-cause infection (aHR 1.46, 95% CI 1.06–2.00 and aHR 1.47, 95% CI 1.00–2.19, respectively); among patients without VRE colonization, Enterococcus domination was associated with excess risk of death or infection (aHR 2.13, 95% CI 1.06–4.29).

Conclusions

Enterococcus status at ICU admission was associated with risk for death or all-cause infection, and rectal carriage of common ICU pathogens predicted specific infections. The gastrointestinal microbiome may have a role in risk stratification and early diagnosis of ICU infections.

Keywords

Critical care Nosocomial infection Vancomycin-resistant Enterococcus Microbiome Colonization resistance Mortality 

Notes

Funding

This research was supported in part by the National Institutes of Health (DK111847) and the American Gastroenterological Association Research Scholar Award (to DEF) and by NIH R01 AI116939 (to ACU).

Compliance with ethical standards

Conflicts of interest

None of the authors have conflicts of interest.

Supplementary material

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Supplementary material 1 (DOCX 24 kb)
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Supplementary material 2 (TIFF 507 kb)
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Supplementary material 3 (TIFF 795 kb)
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Supplementary material 4 (TIFF 795 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature and ESICM 2018

Authors and Affiliations

  • Daniel E. Freedberg
    • 1
  • Margaret J. Zhou
    • 2
  • Margot E. Cohen
    • 3
  • Medini K. Annavajhala
    • 4
  • Sabrina Khan
    • 4
  • Dagmara I. Moscoso
    • 1
  • Christian Brooks
    • 1
  • Susan Whittier
    • 5
  • David H. Chong
    • 6
  • Anne-Catrin Uhlemann
    • 4
    • 7
  • Julian A. Abrams
    • 1
    • 8
  1. 1.Division of Digestive and Liver Diseases, Department of MedicineColumbia University Medical CenterNew YorkUSA
  2. 2.Department of MedicineColumbia University Medical CenterNew YorkUSA
  3. 3.Department of MedicineHospital of the University of PennsylvaniaPhiladelphiaUSA
  4. 4.Microbiome and Pathogen Genomics Core, Department of MedicineColumbia University Medical CenterNew YorkUSA
  5. 5.Division of Laboratory Medicine, Department of Pathology and Cell BiologyColumbia University Medical CenterNew YorkUSA
  6. 6.Division of Pulmonary, Allergy, and Critical Care Medicine, Department of MedicineColumbia University Medical CenterNew YorkUSA
  7. 7.Division of Infectious Diseases, Department of MedicineColumbia University Medical CenterNew YorkUSA
  8. 8.Mailman School of Public HealthNew YorkUSA

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