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Intensive Care Medicine

, 35:678 | Cite as

Increased circulating regulatory T cells (CD4+CD25+CD127) contribute to lymphocyte anergy in septic shock patients

  • Fabienne Venet
  • Chun-Shiang Chung
  • Hakim Kherouf
  • Anne Geeraert
  • Chistophe Malcus
  • Françoise Poitevin
  • Julien Bohé
  • Alain Lepape
  • Alfred Ayala
  • Guillaume Monneret
Original

Abstract

Purpose

Sepsis syndrome represents the leading cause of death in intensive care unit. Patients present features consistent with a decline in immune responsiveness potentially contributing to mortality. We investigated whether CD4+CD25+ regulatory T cells (Treg) participate in the induction of lymphocyte anergy after sepsis.

Method

Observational study in septic shock patients and experimental study in mice.

Results

We took advantage of the recently described flow cytometric gating strategy using the measurement of CD25 and CD127 expressions for monitoring Treg (CD4+CD25+CD127Foxp3+). In patients the increased circulating Treg percentage significantly correlated with a decreased lympho-proliferative response. In a murine model of sepsis mimicking these observations, the ex vivo downregulation of Foxp3 expression using siRNA was associated with a restoration of this response.

Conclusion

The relative increase in circulating Treg might play a role in lymphocyte anergy described after septic shock and represent a standardizable surrogate marker of declining proliferative capacity after sepsis.

Keywords

Septic shock Anergy CD4+CD25+ CD127 Regulatory T cells Foxp3 

Notes

Acknowledgments

Aspects of the work presented here were supported by funds from the Hospices Civils de Lyon (to G.M.) and a grant from the NIH R01 GM46354 (to A.A.). The study was conducted thanks to the logistic support of Centre d’Investigation Clinique (CIC 201) of INSERM and Hospices Civils de Lyon. None of the authors has any potential financial conflict of interest related to this manuscript.

Supplementary material

134_2008_1337_MOESM1_ESM.doc (64 kb)
Electronic supplementary material (DOC 64 kb)

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Copyright information

© Springer-Verlag 2008

Authors and Affiliations

  • Fabienne Venet
    • 1
  • Chun-Shiang Chung
    • 1
  • Hakim Kherouf
    • 2
  • Anne Geeraert
    • 2
  • Chistophe Malcus
    • 2
  • Françoise Poitevin
    • 2
  • Julien Bohé
    • 3
  • Alain Lepape
    • 3
  • Alfred Ayala
    • 1
  • Guillaume Monneret
    • 2
  1. 1.Division of Surgical Research, Rhode Island HospitalBrown UniversityProvidenceUSA
  2. 2.Flow Cytometry Unit, Immunology LaboratoryHôpital E. Herriot, Hospices Civils de LyonLyon Cedex 03France
  3. 3.Intensive Care Units, Centre Hospitalier Lyon-SudHospices Civils de LyonLyonFrance

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