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Bewertung von neuartigen Biomaterialien zum Zweck der Knochenrekonstruktion und -regeneration

In-vitro-Versuche mit Mono- und Kokulturen von Osteoblasten und Endothelzellen

In vitro trials with single and co-cultures of osteoblasts and endothelial cells

Evaluation of new biomaterials for bone reconstruction and regeneration

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Zusammenfassung

Viele verschiedene Knochenersatzstoffe werden für klinische Anwendungen entwickelt. Laut geläufigem Dogma gelten diese Stoffe für die Rekonstruktion und Regeneration von Knochengewebe als geeignet, wenn Osteoblasten und Endothelzellen als Monokulturen oder Kokulturen auf ihnen wachsen und normale Zellfunktionen aufweisen. Jedoch werden auch Tierexperimente vorausgesetzt, um die klinische Eignung der Materialien zu bestätigen. Gute standardisierte In-vitro-Tests dienen also dazu, nur ausgewählte und vielversprechende Stoffe für Tierversuche zuzulassen. Vor allem Kokulturmodelle mit Beteiligung aller relevanter Zelltypen haben ein vielversprechendes Zukunftspotential.

Abstract

Many different types of bone substitute biomaterials are being developed for different applications in the body. The current dogma is that if osteoblasts and endothelial cells grow and exhibit normal cell functions on these materials in vitro as single cultures or in co-cultures, then the biomaterials are suitable for implantation for bone reconstruction and regeneration. Generally, only in vivo animal studies will prove whether this is the case. However, in vitro studies offer a good pre-screening and selection basis to evaluate the biocompatibility of novel biomaterials prior to animal studies. Multicell type co-culture systems hold a great promise for the future.

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Danksagung

Diese Arbeit wurde durch Mittel des Bundesministerium für Bildung und Forschung (Ref. Nr. 0313405C) sowie durch das NoE EXPERTISSUES (Contract No. 500283-2) von der Europäischen Union unterstützt.

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Authors and Affiliations

  1. REPAIR Lab, Institut für Pathologie, Johannes-Gutenberg-Universität, Langenbeckstraße 1, 55133, Mainz, Deutschland

    R.E. Unger, S. Halstenberg, H. Günther, A. Sartoris, C. Brochhausen & C.J. Kirkpatrick

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  1. R.E. Unger
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  2. S. Halstenberg
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  3. H. Günther
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  4. A. Sartoris
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  5. C. Brochhausen
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Correspondence to R.E. Unger.

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Unger, R., Halstenberg, S., Günther, H. et al. Bewertung von neuartigen Biomaterialien zum Zweck der Knochenrekonstruktion und -regeneration. Orthopäde 38, 1020–1028 (2009). https://doi.org/10.1007/s00132-009-1491-x

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