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Cytotoxic and Genotoxic Assessment of Silicon Dioxide Nanoparticles by Allium and Comet Tests

Abstract

Silicon nanoparticles gained a great interest due to its use in biomedical research. It is considered as safe and has been used in nanomedicine. But literature still states its toxicity depending upon the size and dose of silicon nanoparticles. So, current study was aimed to evaluate the cytotoxicity and genotoxicity of silicon dioxide nanoparticles (SiO2NPs) by Allium anaphase–telophase and Comet tests. Characterization of SiO2NPs showed the particle size as 16.12 ± 3.07 nm. The mean diameter of SiO2NPs was having range of 404.66 ± 93.39 nm in solution. Highest total anomalies (18.80 ± 0.45) were observed at 100 µg/mL, whereas least (11.2 ± 0.84) were observed by the 12.5 µg/mL concentration. There was concentration-response association in increased CAs and DNA damage. The highest concentration (100 µg/mL) of SiO2NPs induced the significant DNA damage (149.67 ± 1.15), whereas the least was observed by the negative control (2.67 ± 0.58). The current study revealed the cytotoxic and genotoxic effects of SiO2NPs on the root meristem cells of A. cepa.

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References

  1. Arnold M, Badireddy A, Wiesner M, Di Giulio R, Meyer J (2013) Cerium oxide nanoparticles are more toxic than equimolar bulk cerium oxide in Caenorhabditis elegans Arch Environ Contam Toxicol 65:224–233

  2. Baalousha M et al (2012) Characterization of cerium oxide nanoparticles—Part 2: nonsize measurements. Environ Toxicol Chem 31:994–1003

  3. Barbe C et al (2004) Silica particles: a novel drug-delivery system. Adv Mater 16:1959–1966

  4. Brunner TJ et al (2006) In vitro cytotoxicity of oxide nanoparticles: comparison to asbestos, silica, and the effect of particle solubility. Environ Sci Technol 40:4374–4381

  5. Caritá R, Marin-Morales MA (2008) Induction of chromosome aberrations in the Allium cepa test system caused by the exposure of seeds to industrial effluents contaminated with azo dyes. Chemosphere 72:722–725

  6. Chang J-S, Chang KLB, Hwang D-F, Kong Z-L (2007) In vitro cytotoxicitiy of silica nanoparticles at high concentrations strongly depends on the metabolic activity type of the cell line. Environ Sci Technol 41:2064–2068

  7. Chaparro T, Botta C, Pires E (2010) Biodegradability and toxicity assessment of bleach plant effluents treated anaerobically. Water Sci Technol 62:1312–1319

  8. Chen Y, Chen J, Dong J, Jin Y (2004) Comparing study of the effect of nanosized silicon dioxide and microsized silicon dioxide on fibrogenesis in rats. Toxicol Ind Health 20:21–27

  9. Chen M, von Mikecz A (2005) Formation of nucleoplasmic protein aggregates impairs nuclear function in response to SiO2 nanoparticles. Exp Cell Res 305:51–62

  10. Choi H-S, Kim Y-J, Song M, Song M-K, Ryu J-C (2011) Genotoxicity of nano-silica in mammalian cell lines. Toxicol Environ Health Sci 3:7

  11. Ciğerci İH, Liman R, Özgül E, Konuk M (2015) Genotoxicity of indium tin oxide by Allium and Comet tests. Cytotechnology 67:157–163

  12. Cvjetko P et al (2017) Toxicity of silver ions and differently coated silver nanoparticles in Allium cepa roots. Ecotoxicol Environ Saf 137:18–28

  13. De A, Chakrabarti M, Ghosh I, Mukherjee A (2016) Evaluation of genotoxicity and oxidative stress of aluminium oxide nanoparticles and its bulk form in Allium cepa. Nucleus 59:219–225

  14. Demir E et al (2013) Genotoxicity of different nano-sizes and ions of silica nanoparticles. Fresen Environ Bull 22:2901–2909

  15. Demir E, Kaya N, Kaya B (2014) Genotoxic effects of zinc oxide and titanium dioxide nanoparticles on root meristem cells of Allium cepa by comet assay. Turk J Biol 38:31–39

  16. Demir E, Aksakal S, Turna F, Kaya B, Marcos R (2015) In vivo genotoxic effects of four different nano-sizes forms of silica nanoparticles in Drosophila melanogaster. J Hazard Mater 283:260–266

  17. EI-Ghamery AA, El-Nahas AI, Mansour MM (2000) The action of atrazine herbicide as an inhibitor of cell division on chromosomes and nucleic acids content in root meristems of Allium cepa and Vicia faba. Cytologia 65:277–287

  18. El-Ghamery A, Mousa M (2017) Investigation on the effect of benzyladenine on the germination, radicle growth and meristematic cells of Nigella sativa L. and Allium cepa L. Ann Agric Sci 62:11–21

  19. Eom H-J, Choi J (2009) Oxidative stress of silica nanoparticles in human bronchial epithelial cell Beas-2B. Toxicol In Vitro 23:1326–1332

  20. Gerloff K, Albrecht C, Boots AW, Förster I, Schins RP (2009) Cytotoxicity and oxidative DNA damage by nanoparticles in human intestinal Caco-2 cells. Nanotoxicology 3:355–364

  21. Ghosh M, Chakraborty A, Bandyopadhyay M, Mukherjee A (2011) Multi-walled carbon nanotubes (MWCNT): induction of DNA damage in plant and mammalian cells. J Hazard Mater 197:327–336

  22. Ghosh M, Bhadra S, Adegoke A, Bandyopadhyay M, Mukherjee A (2015) MWCNT uptake in Allium cepa root cells induces cytotoxic and genotoxic responses and results in DNA hyper-methylation. Mutat Res 774:49–58

  23. Gong C, Tao G, Yang L, Liu J, He H, Zhuang Z (2012) The role of reactive oxygen species in silicon dioxide nanoparticle-induced cytotoxicity and DNA damage in HaCaT cells. Mol Biol Rep 39:4915–4925

  24. Gonzalez L et al (2010) Exploring the aneugenic and clastogenic potential in the nanosize range: A549 human lung carcinoma cells and amorphous monodisperse silica nanoparticles as models. Nanotoxicology 4:382–395

  25. Gupta K, Mishra K, Srivastava S, Kumar A (2018) Cytotoxic assessment of chromium and arsenic using chromosomal behavior of root meristem in Allium cepa L. Bull Environ Contam Toxicol 100:803–808

  26. Hidalgo A, Gonzalez-Reyes J, Navas P, Garcia-Herdugo G (1989) Abnormal mitosis and growth inhibition in Allium cepa roots induced by propham chlorpropham. Cytobios 57:7–14

  27. Hirsch LR et al (2003) Nanoshell-mediated near-infrared thermal therapy of tumors under magnetic resonance guidance. Proc Natl Acad Sci USA 100:13549–13554

  28. Hochella MF, Lower SK, Maurice PA, Penn RL, Sahai N, Sparks DL, Twining BS (2008) Nanominerals, mineral nanoparticles, and earth system. Science 319:1631–1635

  29. Jordan MA, Toso RJ, Thrower D, Wilson L (1993) Mechanism of mitotic block and inhibition of cell proliferation by taxol at low concentrations. Proc Natl Acad Sci USA 90:9552–9556

  30. Kaewamatawong T, Shimada A, Okajima M, Inoue H, Morita T, Inoue K, Takano H (2006) Acute and subacute pulmonary toxicity of low dose of ultrafine colloidal silica particles in mice after intratracheal instillation. Toxicol Pathol 34:958–965

  31. Kaygisiz ŞY, Ciğerci İH (2017) Genotoxic evaluation of different sizes of iron oxide nanoparticles and ionic form by SMART, Allium and comet assay. Toxicol Ind Health 33:802–809

  32. Kim Y-J, Yu M, Park H-O, Yang SI (2010) Comparative study of cytotoxicity, oxidative stress and genotoxicity induced by silica nanomaterials in human neuronal cell line. Mol Cell Toxicol 6:336–343

  33. Kumari M, Khan SS, Pakrashi S, Mukherjee A, Chandrasekaran N (2011) Cytogenetic and genotoxic effects of zinc oxide nanoparticles on root cells of Allium cepa. J Hazard Mater 190:613–621

  34. Lee S-W, Kim S-M, Choi J (2009) Genotoxicity and ecotoxicity assays using the freshwater crustacean Daphnia magna and the larva of the aquatic midge Chironomus riparius to screen the ecological risks of nanoparticle exposure. Environ Toxicol Pharm 28:86–91

  35. Liman R (2013) Genotoxic effects of Bismuth (III) oxide nanoparticles by Allium and Comet assay. Chemosphere 93:269–273

  36. Liman R, Ciğerci İH, Öztürk NS (2015) Determination of genotoxic effects of Imazethapyr herbicide in Allium cepa root cells by mitotic activity, chromosome aberration, and comet assay. Pest Biochem Physiol 118:38–42

  37. Lin W, Huang Y-w, Zhou X-D, Ma Y (2006) In vitro toxicity of silica nanoparticles in human lung cancer cells. Toxicol Appl Pharmcol 217:252–259

  38. Lin Y-S, Haynes CL (2010) Impacts of mesoporous silica nanoparticle size, pore ordering, and pore integrity on hemolytic activity. J Am Chem Soc 132:4834–4842

  39. Mangalampalli B, Dumala N, Grover P (2018) Allium cepa root tip assay in assessment of toxicity of magnesium oxide nanoparticles and microparticles. J Environ Sci 66:125–137

  40. Moghimi SM, Hunter AC, Murray JC (2005) Nanomedicine: current status and future prospects. FASEB J 19:311–330

  41. Mu Q, Hondow NS, Krzemiński Ł, Brown AP, Jeuken LJ, Routledge MN (2012) Mechanism of cellular uptake of genotoxic silica nanoparticles. Part Fibre Toxicol 9:29

  42. Napierska D, Thomassen LC, Lison D, Martens JA, Hoet PH (2010) The nanosilica hazard: another variable entity. Part Fibre Toxicol 7:39

  43. Nel A, Xia T, Mädler L, Li N (2006) Toxic potential of materials at the nanolevel. Science 311:622–627

  44. Oberdörster G, Oberdörster E, Oberdörster J (2005) Nanotoxicology: an emerging discipline evolving from studies of ultrafine particles. Environ Health Perspect 113:823–839

  45. Park MV et al (2009) In vitro developmental toxicity test detects inhibition of stem cell differentiation by silica nanoparticles. Toxicol Appl Pharmcol 240:108–116

  46. Rahman MM, Rahman MF, Nasirujjaman K (2017) A study on genotoxicity of textile dyeing industry effluents from Rajshahi, Bangladesh, by the Allium cepa test. Chem Ecol 33:434–446

  47. Rajeshwari A, Roy B, Chandrasekaran N, Mukherjee A (2016) Cytogenetic evaluation of gold nanorods using Allium cepa test. Plant Physiol Biochem 109:209–219

  48. Rodea-Palomares I, Boltes K, Fernández-Pinas F, Leganés F, García-Calvo E, Santiago J, Rosal R (2010) Physicochemical characterization and ecotoxicological assessment of CeO2 nanoparticles using two aquatic microorganisms. Toxicol Sci 119:135–145

  49. Rothen-Rutishauser BM, Schürch S, Haenni B, Kapp N, Gehr P (2006) Interaction of fine particles and nanoparticles with red blood cells visualized with advanced microscopic techniques. Environ Sci Technol 40:4353–4359

  50. Saxena PN, Chauhan LK, Gupta SK (2005) Cytogenetic effects of commercial formulation of cypermethrin in root meristem cells of Allium sativum: Spectroscopic basis of chromosome damage. Toxicology 216:244–252

  51. Sayes CM, Reed KL, Warheit DB (2007) Assessing toxicity of fine and nanoparticles: comparing in vitro measurements to in vivo pulmonary toxicity profiles. Toxicol Sci 97:163–180

  52. Sharma S, Vig AP (2012) Genotoxicity of atrazine, avenoxan, diuron and quizalofop-P-ethyl herbicides using the Allium cepa root chromosomal aberration assay. Terrest Aquat Environ Toxicol 6:90–95

  53. Singh D, Roy BK (2017) Evaluation of malathion-induced cytogenetical effects and oxidative stress in plants using Allium test. Acta Physiol Plant 39:92

  54. Slowing II, Vivero-Escoto JL, Wu C-W, Lin VS-Y (2008) Mesoporous silica nanoparticles as controlled release drug delivery and gene transfection carrier. Adv Drug Deliv Rev 60:1278–1288

  55. Sudhakar R, KN NG, Venu G (2001) Mitotic abnormalities induced by silk dyeing industry effluents in the cells of Allium cepa. Cytologia 66:235–239

  56. Tice RR, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H, Miyamae Y, Rojas E, Ryu JC, Sasaki YF (2000) Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen 35:206–221

  57. Türkoğlu Ş (2015) Evaluation of genotoxic effects of five flavour enhancers (glutamates) on the root meristem cells of Allium cepa. Toxicol Ind Health 31:792–801

  58. Wagner S, Münzer S, Behrens P, Scheper T, Bahnemann D, Kasper C (2009) Cytotoxicity of titanium and silicon dioxide nanoparticles. Journal of physics: conference series, vol 1. IOP Publishing, Bristol, p 012022

  59. Xue Z et al (2006) Biotoxicology and biodynamics of silica nanoparticle. J Cent South Univ Med Sci 31:6–8

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Correspondence to Muhammad Muddassir Ali.

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Liman, R., Acikbas, Y., Ciğerci, İ.H. et al. Cytotoxic and Genotoxic Assessment of Silicon Dioxide Nanoparticles by Allium and Comet Tests. Bull Environ Contam Toxicol 104, 215–221 (2020). https://doi.org/10.1007/s00128-020-02783-3

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Keywords

  • Silicon dioxide nanoparticles
  • Mitotic index
  • DNA damage
  • Allium cepa