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Synergistic Effect of Zinc Oxide Nanoparticles and Heat Stress on the Alleviation of Transcriptional Gene Silencing in Arabidopsis thaliana

  • Jingjing Wu
  • Ting WangEmail author
Article

Abstract

Phytotoxicity is an inevitable consideration in evaluating the potential ecological effects of nanoparticles (NPs). Natural ecosystems are complex and accompanied by many other environmental factors. Thus understanding the impact of NPs on plant response to other environmental stresses is crucial to assess the comprehensive toxicity of NPs in ecosystem. In the present study, Arabidopsis thaliana seedlings were cultured in medium containing zinc oxide NPs (ZnO-NPs) then subjected to heat stress at 37°C. Alleviation of transcriptional gene silencing (TGS) in aerial leafy tissues was assessed as an epi-genotoxic endpoint. Results showed that 1 µg/mL ZnO-NPs alone can not alleviate GUS gene (β-glucuronidase) which silenced by TGS (TGS-GUS), but it significantly enhanced heat stress-induced alleviation of TGS-GUS, suggesting an synergistic effect of ZnO-NPs and heat stress on genomic instability. Further study showed that the initiation of synergistic effect could be regulated by plant developmental stage, heat duration and temperature, and heat shock related genes might be involved in.

Keywords

Synergistic effect Zinc oxide nanoparticles Heat stress Transcriptional gene silencing Arabidopsis thaliana 

Notes

Acknowledgements

We thank Dr. Ortrun Mittelsten Scheid and NASC for their generous provision of the various A. thaliana seeds. This work was supported by the National Natural Science Foundation of China (11575233), and the Youth Innovation Promotion Association of Chinese Academy of Sciences (2017485).

Compliance with Ethical Standards

Conflict of interest

The authors declare no competing financial interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical ScienceChinese Academy of SciencesHefeiPeople’s Republic of China
  2. 2.University of Science and Technology of ChinaHefeiPeople’s Republic of China

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