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Accumulation and Depuration of Nonylphenol and Its Effect on the Expressions of Vitellogenin and Vitellogenin Receptor in Freshwater Prawn Macrobrachium rosenbergii


Accumulation of nonylphenol (NP) in hepatopancreas, gonad, eyestalk, and muscle of freshwater prawn Macrobrachium rosenbergii following 72 h exposure to 100 µg/L NP, and depuration of NP in these tissues at 0.5–192 h post exposure were examined. We also examined the expressions of vitellogenin (Vg) and vitellogenin receptor (VgR) of prawn following 0–20 days exposure to 0, 1, 10, and 100 µg/L NP. NP accumulation in hepatopancreas and gonad with high concentration, and low concentration in muscle, but depurated faster in eyestalk and muscle. The expressions of vitellogenin (Vg) and vitellogenin receptor (VgR) increased directly with dose and time. In conclusion, NP accumulated significantly in gonad together with high Vg and VgR expressions, and depurated slow in hepatopancreas and gonad when prawns were removed back to control water. The induction of Vg and VgR under NP exposure might be a stress response in M. rosenbergii.

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  1. Ara F, Damrongphol P (2014) Vitellogenin gene expression at different ovarian stages in the giant freshwater prawn, Macrobrachium rosenbergii, and stimulation by 4-nonylphenol. Aquac Res 45(2):320–326

  2. Bai H, Qiao H, Li F, Fu H, Jiang S, Zhang W, Yan Y, Xiong Y, Sun S, Jin S, Gong Y, Wu Y (2016) Molecular and functional characterization of the vitellogenin receptor in oriental river prawn, Macrobrachium nipponense. Comp Biochem Physiol A 194:45–55

  3. Baldwin WS, Graham SE, Shea D, LeBlanc GA (2009) Metabolic androgenization of female Daphnia magna by the xenoestrogen 4-nonylphenol. Environ Toxicol Chem 16(9):1905–1911

  4. Bodin N, Abarnou A, Le Guellec AM, Loizeau V, Philippon X (2007) Organochlorinated contaminants in decapod crustaceans from the coasts of Brittany and Normandy (France). Chemosphere 67(9):S36–S47

  5. Boulangé-Lecomte C, Xuereb B, Trémolet G, Duflot A, Giusti N, Olivier S, Legrand E, Forget-Leray J (2017) Controversial use of vitellogenin as a biomarker of endocrine disruption in crustaceans: new adverse pieces of evidence in the copepod Eurytemora affinis. Comp Biochem Physiol C 201:66–75

  6. Fernandes D, Loi B, Porte C (2011) Biosynthesis and metabolism of steroids in molluscs. J Steroid Biochem Mol Biol 127(3):189–195

  7. Forget-Leray J, Landriau I, Minier C, Leboulenger F (2005) Impact of endocrine toxicants on survival, development, and reproduction of the estuarine copepod Eurytemora affinis (Poppe). Ecotoxicol Environ Saf 60(3):288–294

  8. Gatidou G, Vassalou E, Thomaidis NS (2010) Bioconcentration of selected endocrine disrupting compounds in the Mediterranean mussel, Mytilus galloprovincialis. Mar Pollut Bull 60(11):2111–2116

  9. Gautam GJ, Chaube R, Joy K (2015) Toxicity and tissue accumulation of 4-nonylphenol in the catfish Heteropneustes fossilis with a note on prevalence of 4-NP in water samples. Endocr Disrupt 3(1):1–12

  10. Grilo TF, Cardoso PG, Pato P, Duarte AC, Pardal MA (2014) Uptake and depuration of PCB-153 in edible shrimp Palaemonetes varians and human health risk assessment. Ecotoxicol Environ Saf 101:97–102

  11. Guo H, Miao YT, Xian JA, Qian K, Wang AL (2015) Expression profile of antioxidant enzymes in hemocytes from freshwater prawn Macrobrachium rosenbergii exposed to an elevated level of copper. Bull Environ Contam Toxicol 95(4):447–451

  12. Harris CA, Santos EM, Janbakhsh A, Pottinger TG, Tyler CR, Sumpter JP (2001) Nonylphenol affects gonadotropin levels in the pituitary gland and plasma of female rainbow trout. Environ Sci Technol 35(14):2909–2916

  13. He TT, Liang B, Liu WH, Shin PKS, Wu RSS (2012) Estrogenic potential of benzotriazole on marine medaka (Oryzias melastigma). Ecotoxicol Environ Saf 80:327–332

  14. Hutchinson TH (2002) Reproductive and developmental effects of endocrine disrupters in invertebrates: in vitro and in vivo approaches. Toxicol Lett 131(1):75–81

  15. Jubeaux G, Simon R, Salvador A, Quéau H, Chaumot A, Geffard O (2012) Vitellogenin-like proteins in the freshwater amphipod Gammarus fossarum (Koch, 1835): functional characterization throughout reproductive process, potential for use as an indicator of oocyte quality and endocrine disruption biomarker in males. Aquat Toxicol 112–113:72–82

  16. Lafontaine A, Hanikenne M, Boulangé-Lecomte C, Forget-Leray J, Thomé JP, Gismondi E (2016) Vitellogenin and vitellogenin receptor gene expression and 20-hydroxyecdysone concentration in Macrobrachium rosenbergii exposed to chlordecone. Environ Sci Pollut R 23(20):20661–20671

  17. Lepretti M, Paolella G, Giordano D, Marabotti A, Gay F, Capaldo A, Esposito C, Caputo I (2015) 4-Nonylphenol reduces cell viability and induces apoptosis and ER-stress in a human epithelial intestinal cell line. Toxicol In Vitro 29(7):1436–1444

  18. Liang X, Wang M, Chen X, Zha J, Chen H, Zhu L, Wang Z (2014) Endocrine disrupting effects of benzotriazole in rare minnow (Gobiocypris rarus) in a sex-dependent manner. Chemosphere 112:154–162

  19. Loos R, Hanke G, Umlauf G, Eisenreich SJ (2007) LC-MS-MS analysis and occurrence of octyl- and nonylphenol, their ethoxylates and their carboxylates in Belgian and Italian textile industry, waste water treatment plant effluents and surface waters. Chemosphere 66(4):690–699

  20. Lye CM, Bentley MG, Galloway T (2008) Effects of 4-nonylphenol on the endocrine system of the shore crab, Carcinus maenas. Environ Toxicol 23(3):309–318

  21. Salgueiro-González N, Concha-Graña E, Turnes-Carou I, Muniategui-Lorenzo S, López-Mahía P, Prada-Rodríguez D (2012) Determination of alkylphenols and bisphenol A in seawater samples by dispersive liquid-liquid microextraction and liquid chromatography tandem mass spectrometry for compliance with environmental quality standards (Directive 2008/105/EC). J Chromatogr A 1223:1–8

  22. Short S, Yang G, Kille P, Ford AT (2014) Vitellogenin is not an appropriate biomarker of feminisation in a Crustacean. Aquat Toxicol 153:89–97

  23. Smith MD, Hill EM (2006) Profiles of short chain oligomers in roach (Rutilus rutilus) exposed to waterborne polyethoxylated nonylphenols. Sci Total Environ 356(1–3):100–111

  24. Tatem HE (1986) Bioaccumulation of polychlorinated biphenyls and metals from contaminated sediment by freshwater prawns, Macrobrachium rosenbergii and clams, Corbicula fluminea. Arch Environ Contam Toxicol 15(2):171–183

  25. Tiu SHK, Benzie J, Chan SM (2008) From hepatopancreas to ovary: molecular characterization of a shrimp vitellogenin receptor involved in the processing of vitellogenin. Biol Reprod 79(1):66–74

  26. Vega-Morales T, Sosa-Ferrera Z, Santana-Rodríguez JJ (2010) Determination of alkylphenol polyethoxylates, bisphenol-A, 17α-ethynylestradiol and 17β-estradiol and its metabolites in sewage samples by SPE and LC/MS/MS. J Hazard Mater 183(1):701–711

  27. Wu B, Liu Z, Zhou L, Ji G, Yang A (2015) Molecular cloning, expression, purification and characterization of vitellogenin in scallop Patinopecten yessoensis with special emphasis on its antibacterial activity. Dev Comp Immunol 49(2):249–258

  28. Yılmaz AB, Yılmaz L (2007) Influences of sex and seasons on levels of heavy metals in tissues of green tiger shrimp (Penaeus semisulcatus de Han, 1844). Food Chem 101(4):1664–1669

  29. Zhang S, Wang S, Li H, Li L (2011) Vitellogenin, a multivalent sensor and an antimicrobial effector. Int J Biochem Cell 43(3):303–305

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This research was supported by the National Natural Science Foundation of China (31600321) and Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams (No. 2019KJ149). We are especially grateful for the critical comments from the anonymous reviewers.

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Correspondence to Chun-hua Zhu.

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Guo, H., Chen, L., Li, G. et al. Accumulation and Depuration of Nonylphenol and Its Effect on the Expressions of Vitellogenin and Vitellogenin Receptor in Freshwater Prawn Macrobrachium rosenbergii. Bull Environ Contam Toxicol 103, 729–733 (2019).

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  • Nonylphenol
  • Bioaccumulation
  • Depuration
  • Vitellogenin
  • Vitellogenin receptor
  • Macrobrachium rosenbergii