Advertisement

Effects of Copper on Root Morphology, Cations Accumulation, and Oxidative Stress of Grapevine Seedlings

  • Kai-Wei Juang
  • Yu-Ching Lo
  • Tzu-Hsuan Chen
  • Bo-Ching ChenEmail author
Article

Abstract

In the present study, a hydroponic experiment was conducted to investigate the oxidative stress and the copper (Cu) accumulation in grapevines exposed to three Cu levels (0, 5, and 15 µM) for 1, 2, and 3 days. The results showed that the root elongation was stunted at the highest-exposure concentration. The Cu accumulation in the grapevines increased with increasing Cu treatments, while the other nutrient elements (Ca, Mg and K) absorbed by the grapevines decreased. Most of the Cu taken up by the grapevines was accumulated in the roots. Compared to the data for 1 day after treatment, the Cu-addition significantly decreased the Mg and K concentration in the roots and leaves, yet increased the superoxide dismutase activity in the leaves after 3 days of treatment. For the reactive oxygen species, the malondialdehyde increased with increasing Cu levels in the roots and leaves; however, both the Cu-addition and exposure duration reduced the H2O2 level in the root. Additionally, the Cu-induced accumulation of ·O2 and H2O2 in the grapevine leaves can be observed by the histochemical staining of nitroblue tetrazolium and diaminobenzidine, respectively. In conclusion, the present results indicate that excess Cu results in a change of the root morphology and leads to oxidative stress for the grapevine leaves and roots.

Keywords

Antioxidant enzyme Copper Grapevine seedlings Oxidative stress Reactive oxygen species Root elongation 

Notes

Acknowledgments

This study was financed by the Ministry of Science and Technology, Taiwan under Grant Nos. MOST 105-2313-B-343-001 and MOST 106-2313-B-343-001.

References

  1. Ambrosini VG, Rosa DJ, de Melo GWB, Zalamena J, Cella C, Simao DG, da Silva LS, dos Santos HP, Toselli M, Tiecher TL, Brunetto G (2018) High copper content in vineyard soils promotes modifications in photosynthetic parameters and morphological changes in the root system of ‘Red Niagara’ plantlets. Plant Physiol Biochem 128:89–98CrossRefGoogle Scholar
  2. Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287CrossRefGoogle Scholar
  3. Cambrolle J, Garcia JL, Figueroa ME, Cantos M (2015) Evaluating wild grapevine tolerance to copper toxicity. Chemosphere 120:171–178CrossRefGoogle Scholar
  4. Chen PY, Lee YI, Chen BC, Juang KW (2013) Effects of calcium on rhizotoxicity and the accumulation and translocation of copper by grapevines. Plant Physiol Biochem 73:375–382CrossRefGoogle Scholar
  5. Ferreira PAA, Marchezan C, Ceretta CA, Tarouco CP, Lourenzi CR, Silva LS, Soriani HH, Nicoloso FT, Cesco S, Mimmo T, Brunetto G (2018) Soil amendment as a strategy for the growth of young vines when replanting vineyards in soils with high copper content. Plant Physiol Biochem 126:152–162CrossRefGoogle Scholar
  6. Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidants machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 48:909–930CrossRefGoogle Scholar
  7. Heath RL, Packer L (1968) Photoperoxidation in isolate chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198CrossRefGoogle Scholar
  8. Jana S, Choudhuri MA (1981) Glycolate metabolism of three submerged aquatic angiosperms during aging. Aquat Bot 12:345–354CrossRefGoogle Scholar
  9. Juang KW, Lai HY, Chen BC (2011) Coupling bioaccumulation and phytotoxicity to predict copper removal by switchgrass grown hydroponically. Ecotoxicology 20:827–835CrossRefGoogle Scholar
  10. Juang KW, Lee YI, Lai HY, Wang CH, Chen BC (2012) Copper accumulation, translocation, and toxic effects in grapevine cuttings. Environ Sci Pollut Res 19:1315–1322CrossRefGoogle Scholar
  11. Juang KW, Lee YI, Lai HY, Chen BC (2014) Influence of magnesium on copper phytotoxicity to and accumulation and translocation in grapevines. Ecotoxicol Environ Saf 104:36–42CrossRefGoogle Scholar
  12. Kopittke PM, Blamey FPC, Menzies NW (2008) Toxicities of soluble Al, Cu, and La include ruptures to rhizodermal and root cortical cells of cowpea. Plant Soil 303:217–227CrossRefGoogle Scholar
  13. Kopittke PM, Blamey FPC, Asher CJ, Menzies NW (2010) Trace metal phytotoxicity in solution culture: a review. J Exp Bot 61:945–954CrossRefGoogle Scholar
  14. Liu D, Kottke I (2004) Subcellular localization of copper in the root cells of Allium sativum by electron energy loss spectroscopy (EELS). Bioresour Technol 94:153–158CrossRefGoogle Scholar
  15. Liu D, Chen J, Mahmood Q, Li S, Wu J, Ye Z, Peng D, Yan W, Lu K (2014) Effects of Zn toxicity on root morphology, ultrastructure, and the ability to accumulate Zn in Moso bamboo (Phyllostachys pubescens). Environ Sci Pollut Res 21:13615–13624CrossRefGoogle Scholar
  16. Miotto A, Ceretta CA, Brunetto G, Nicoloso FT, Girotto E, Farias JG, Tiecher TL, de Conti L, Trentin G (2014) Copper uptake, accumulation and physiological changes in adult grapevines in response to excess copper in soil. Plant Soil 374:593–610CrossRefGoogle Scholar
  17. Mirlean N, Roisenberg A, Chies JO (2007) Metal contamination of vineyard soils in wet subtropics (Southern Brazil). Environ Pollut 149:10–17CrossRefGoogle Scholar
  18. Mostofa MG, Seraj ZI, Fujita M (2014) Exogenous sodium nitroprusside and glutathione alleviate copper toxicity by reducing copper uptake and oxidative damage in rice (Oryza sativa L.) seedlings. Protoplasma 251:1373–1386CrossRefGoogle Scholar
  19. Perez-de-los-Reyes C, Ortiz-Villajos JAA, Navarro FJG, Martin-Consuegra SB, Ballesta RJ (2013) Grapevine leaf uptake of mineral elements influenced by sugar foam amendment of an acidic soil. Vitis 52:157–164Google Scholar
  20. Rossini Oliva S, Mingorance MD, Valdes B, Leidi EO (2010) Uptake, localisation and physiological changes in response to cooper excess in Erica andevalensis. Plant Soil 328:411–420CrossRefGoogle Scholar
  21. Thounaojam TC, Panda P, Mazumdar P, Kumar D, Sharma GD, Sahoo L, Panda SK (2012) Excess copper induced oxidative stress and responses of antioxidants in rice. Plant Physiol Biochem 53:33–39CrossRefGoogle Scholar
  22. Thounaojam TC, Panda P, Choudhury S, Patra HK, Panda SK (2014) Zinc ameliorates copper-induced oxidative stress in developing rice (Oryza sativa L.) seedlings. Protoplasma 251:61–69CrossRefGoogle Scholar
  23. Tiecher TL, Ceretta CA, Ferreira PAA, Lourenzi CR, Tiecher T, Girotto E, Nicoloso FT, Soriani HH, de Conti L, Mimmo T, Cesco S, Brunetto G (2016) The potential of Zea mays L. in remediating copper and zinc contaminated soils for grapevine production. Geoderma 262:52–61CrossRefGoogle Scholar
  24. Tiecher TL, Tiecher T, Ceretta CA, Ferreira PAA, Nicoloso FT, Soriani HH, de Conti L, Kulmann MSS, Schneider RO, Brunetto G (2017) Tolerance and translocation of heavy metals in young grapevine (Vitis vinifera) grown in sandy acidic soil with interaction of high doses of copper and zinc. Sci Hortic 222:203–212CrossRefGoogle Scholar
  25. Tiecher TL, Soriani HH, Tiecher T, Ceretta CA, Nicoloso FT, Tarouco CP, Clasen BE, De Conti L, Tassinari A, Melo GWB, Brunetto G (2018) The interaction of high copper and zinc doses in acid soil changes the physiological state and development of the root system in young grapevines (Vitis vinifera). Ecotoxicol Environ Saf 148:985–994CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of AgronomyNational Chiayi UniversityChiayiTaiwan
  2. 2.Master Program of Green Technology for SustainabilityNanhua UniversityChiayiTaiwan

Personalised recommendations