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Identification of Differentially Expressed Genes of Rice Under Cadmium Stress Using DDRT-PCR Approach

  • Jian-hui Wang
  • Hai-Ming Zhao
  • Ce-hui MoEmail author
  • Xue-wen HouEmail author
Article

Abstract

Cadmium (Cd) is one of the hazardous environmental pollutants, and it can be harmful to human health through consumption of food-plants capable of bioaccumulating Cd. Therefore, lowering cadmium accumulation in plants is highly desirable. Here, a rice cultivar ‘Qisanzhan’ was studied using differential display reverse transcription-polymerase chain reaction (DDRT-PCR). Fifty-six differentially expressed genes were found in the root tips of 4-leaf stage rice seedlings exposed to 4 and 12 h of 50 µmol/L Cd(NO3)2 in a nutrient solution using DDRT-PCR. Further validation using semi-quantitative RT-PCR showed that the expression patterns of 16 genes were consistent with those found in DDRT-PCR. These genes encode receptor-like protein kinase, pleiotropic drug resistance protein, aquaporin protein, plasma membrane ATPase, etc. The differentially genes identified here can be used to obtain a better understanding of the molecular mechanisms of Cd absorption and accumulation in plants.

Keywords

Cadmium DDRT-PCR Differential gene expression Rice sqRT-PCR 

Notes

Acknowledgements

We sincerely thank Dr. David Leung (University of Canterbury, New Zealand) for his critical reading and editing of this manuscript. This work was sponsored by the Research Team Project of the Natural Science Foundation of Guangdong Province (2016A030312009), NSFC-Guangdong Joint Fund (U1501233), the Science and Technology Program of Guangzhou, China (201707010032) and the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry, China ([2007]1108).

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresourcesSouth China Agricultural UniversityGuangzhouChina
  2. 2.Guangdong Provincial Research Center for Environment Pollution Control and Remediation Materials, College of Life Science and TechnologyJinan UniversityGuangzhouChina

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