Effects of Urban–rural Atmospheric Environment on Heavy Metal Accumulation and Resistance Characteristics of Pinus tabulaeformis in Northern China

  • Ruirui Zhao
  • Tong Yang
  • Cong Shi
  • Meili Zhou
  • Guoping Chen
  • Fuchen ShiEmail author


The washed and unwashed current (C) and previous year (C + 1) needles, branches and top soils of Pinus tabulaeformis trees were sampled at five sites Haitai industrial district (HT), Puji River (PJ), Fukang road (FK), Residential area (RA) and Baxian Mountain (BX) in Tianjin along an urban–rural gradient and analyzed for heavy metals (Cu, Mn, Zn, Pb and Cd) concentrations via ICP. C + 1 needles generally had higher Mn, Pb, Cd than C needles while the opposite was for Cu and Zn. Total Cu, Zn, Pb, Cd in soils peaked at HT and decreased at RA and BX. Heavy metals were generally higher in the unwashed needles than the washed needles at all sites. Meanwhile MDA, soluble sugar and free proline concentration in needles were increased with the increasing of heavy metal contents along the urban–rural gradient, further correlated with the heavy metal contents.


Pinus tabulaeformis Heavy metals Needle ages Resistance indexes 



This work was funded by Nankai University, College of Life Sciences, as well as the project of Tianjin Agricultural Environmental Monitoring Station (No. 2017SJ004).


  1. Al-Alawi MM, Mandiwana KL (2007) The use of Aleppo pine needles as a bio-monitor of heavy metals in the atmosphere. J Hazard Mater 148:43–46CrossRefGoogle Scholar
  2. Al-Khashman OA (2004) Heavy metal distribution in dust and soils from the work place in Korak Industrial Estate, Jordan. Atmos Environ 38:6803–6812CrossRefGoogle Scholar
  3. Aznar JC, Richer-Laflèche M, Bégin C, Bégin Y (2009) Lead exclusion and copper translocation in black spruce Needles. Water Air Soil Pollut 203:139–145CrossRefGoogle Scholar
  4. Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39:205–207CrossRefGoogle Scholar
  5. Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287CrossRefGoogle Scholar
  6. Celik A, Kartal AA, Akdoğan A, Kaska Y (2005) Determining the heavy metal pollution in Denizli (Turkey) by using Robinio pseudo-acacia L. Environ Int 31:105–112CrossRefGoogle Scholar
  7. Chen P, Zhang J, Bi X, Feng Y, Wu J (2013) Pollution characteristics and sources of heavy metals in PM10 and PM2.5 in Tianjin City. Acta Sci Nat Univ Nankaiensis 46:1–7 (in Chinese)Google Scholar
  8. Gu J, Bai Z, Li W, Wu L, Liu A, Dong H, Xie Y (2011) Chemical composition of PM2.5 during winter in Tianjin, China. Particuology 9:215–221CrossRefGoogle Scholar
  9. Huang D, Xu J, Zhang S (2012) Valuing the health risks of particulate air pollution in the Pearl River Delta, China. Environ Sci Policy 15:38–47CrossRefGoogle Scholar
  10. Hwang HJ, Yook SJ, Ahn KH (2011) Experimental investigation of submicron and ultrafine soot particle removal by tree leaves. Atmos Environ 45:6987–6994CrossRefGoogle Scholar
  11. Iii FSC, Kedrowski RA (1983) Seasonal changes in nitrogen and phosphorus fractions and autumn retranslocation in evergreen and deciduous taiga trees. Ecology 64:376–391CrossRefGoogle Scholar
  12. Kayama M, Sasa K, Koike T (2002) Needle life span, photosynthetic rate and nutrient concentration of Picea glehnii, P. jezoensis and P. abies planted on serpentine soil in northern Japan. Tree Physiol 22:707–716CrossRefGoogle Scholar
  13. Kingston HM (1996) Microwave assisted acid digestion of siliceous and or ganically-based matrices, method 3052. United States Environmental Protection Agency, Washington, DC, USAGoogle Scholar
  14. Kuang YW, Wen DZ, Zhou GY, Liu SZ (2007) Distribution of elements in needles of Pinus massoniana (Lamb.) was uneven and affected by needle age. Environ Pollut 145:146–153CrossRefGoogle Scholar
  15. Liu G (1996) Soil physical and chemical analysis & description of soil profiles. Standards Press of China, Beijing (in Chinese)Google Scholar
  16. Nicola FD, Maisto G, Prati MV, Alfani A (2008) Leaf accumulation of trace elements and polycyclic aromatic hydrocarbons (PAHs) in Quercus ilex L. Environ Pollut 153:376CrossRefGoogle Scholar
  17. Nouri J, Khorasani N, Lorestani B, Karami M, Hassani AH, Yousefi N (2009) Accumulation of heavy metals in soil and uptake by plant species with phytoremediation potential. Environ Earth Sci 59:315–323CrossRefGoogle Scholar
  18. Pietrzykowski M, Socha J, van Doorn NS (2014) Linking heavy metal bioavailability (Cd, Cu, Zn and Pb) in Scots pine needles to soil properties in reclaimed mine areas. Sci Total Environ 470–471:501–510CrossRefGoogle Scholar
  19. Raghunath R, Tripathi RM, Kumar AV, Sathe AP, Khandekar RN, Nambi KS (1999) Assessment of Pb, Cd, Cu, and Zn exposures of 6–10 year-old children in Mumbai. Environ Res 80:215CrossRefGoogle Scholar
  20. Rothpfeffer C, Karltun E (2007) Inorganic elements in tree compartments of Picea abies —concentrations versus stem diameter in wood and bark and concentrations in needles and branches. Biomass Bioenerg 31:717–725CrossRefGoogle Scholar
  21. Sameckacymerman A, Stankiewicz A, Kolon K, Kempers AJ (2009) Self-organizing feature map (neural networks) as a tool to select the best indicator of road traffic pollution (soil, leaves or bark of Robinia pseudoacacia L.). Environ Pollut 157:2061–2065CrossRefGoogle Scholar
  22. Serbula SM, Kalinovic T, Ilic A, Kalinovic J, Steharnik M (2013) Assessment of airborne heavy metal pollution using Pinus spp. and Tilia spp. Aerosol Air Qual Res 13:563–573CrossRefGoogle Scholar
  23. Shao L, Xiao H, Daishe WU (2013) Speciation of heavy metals in airborne particles, road dusts, and soils along expressways in China. Acta Geochim 32:420–429Google Scholar
  24. Shi C, Watanabe T, Koike T (2017) Leaf stoichiometry of deciduous tree species in different soils exposed to free-air O3 enrichment over two growing seasons. Environ Exp Bot 138:148–163CrossRefGoogle Scholar
  25. Sun F, Wen D, Kuang Y, Li J, Li J, Zuo W (2010) Concentrations of heavy metals and polycyclic aromatic hydrocarbons in needles of Masson pine (Pinus massoniana L.) growing nearby different industrial sources. J Environ Sci 22:1006–1013CrossRefGoogle Scholar
  26. Tan J, Duan J, He K, Ma Y, Duan F, Chen Y, Fu J (2009) Chemical characteristics of PM2.5 during a typical haze episode in Guangzhou. J Environ Sci 21:774–781CrossRefGoogle Scholar
  27. Uddin I, Bano A, Masood S (2015) Chromium toxicity tolerance of Solanum nigrum L. and Parthenium hysterophorus L. plants with reference to ion pattern, antioxidation activity and root exudation. Ecotoxicol Environ Safe 113:271–278CrossRefGoogle Scholar
  28. Wang Q et al (2015) Probing the severe haze pollution in three typical regions of China: characteristics, sources and regional impacts. Atmos Environ 120:76–88CrossRefGoogle Scholar
  29. Yilmaz S, Zengin M (2004) Monitoring environmental pollution in Erzurum by chemical analysis of Scots pine (Pinus sylvestris L.) needles. Environ Int 29:1041–1047CrossRefGoogle Scholar

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Ruirui Zhao
    • 1
  • Tong Yang
    • 1
  • Cong Shi
    • 2
  • Meili Zhou
    • 1
  • Guoping Chen
    • 1
  • Fuchen Shi
    • 1
    Email author
  1. 1.College of Life SciencesNankai UniversityTianjinChina
  2. 2.School of Environment and EnergyShenzhen Graduate School of Peking UniversityShenzhenChina

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