Effects of Copper Oxide Nanoparticles on Tissue Accumulation and Antioxidant Enzymes of Galleria mellonella L.
Effects of copper oxide nanoparticles (CuO NPs) were investigated in the midgut and fat body of Galleria mellonella. Fourth instar larvae were exposed to 10 µg Cu/L of CuO until becoming last instar larvae, and catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione-s-transferase (GST) and acetylcholinesterase (AChE) and metal accumulation were evaluated. Copper accumulation was observed in midgut and fat body tissues of G. mellonella larvae exposed to CuO NPs. CuO NPs increased CAT activities in midgut and fat body, while SOD activities were decreased. CuO NPs exhibited significant increases in GST activity in fat body, while no significant differences were observed in the midgut of G. mellonella larvae. AChE activity significantly decreased in the midgut of G. mellonella whereas there is no significant effect on fat body in CuO NPs exposed larvae. In overall, these findings demonstrate that tissue accumulation and oxidative stress that is countered by antioxidant enzymes occur when G. mellonella larvae exposed to environmental concentration of CuO nanoparticles.
KeywordsAcetylcholinesterase Antioxidant enzymes Copper oxide nanoparticles Galleria mellonella Glutathione-s-transferase
We would like to thank to Prof.Dr. Cahit Erdem.
- Abd El Wahab RA, Anwar EM (2014) The effect of direct and indirect use of nanoparticles on cotton leaf worm, Spodoptera littoralis. Int J Chem Biol Sci 1(7):17–24Google Scholar
- Bronksill JF (1961) A cage to simplify the rearing of the greater wax moth, Galleria mellonella (Pyralidae). J Lep Soc 15:102–104Google Scholar
- Fouad H, Hongjie L, Hosni D, Wei J, Abbas G, Ga’al H, Jianchu M (2018) Controlling Aedes albopictus and Culex pipiens pallens using silver nanoparticles synthesized from aqueous extract of Cassia fistula fruit pulp and its mode of action. Artif Cells Nanomed Biotechnol 46:558–567CrossRefGoogle Scholar
- Greenwald RA (1985) Handbook of methods for oxygen radical research. CRC Press, Boca RatonGoogle Scholar
- Habig WH, Pabst MJ, Jakoby WB (1974) Glutathione-s-transferases, the first enzymatic step in mercapturic acid formation. J Biol Chem 249:7130–7139Google Scholar
- Kafel A, Bednarska K, Augustyniak M, Witas I, Szulińska E (2003) Activity of glutathione transferase in Spodoptera exigua larvae exposed to cadmium and zinc in two subsequent generations. Environ 28:683–686Google Scholar
- Langston WJ, Bebianno MJ, Burt GR (1998) Metal handling strategies in molluscs. In Metal metabolism in aquatic environments, pp 219–283. Kluwer Academic Publishers, NorwellGoogle Scholar
- Liu J, Fand D, Wang L, Shi L, Ding J, Chen Y, Shen S (2014) Effects of ZnO, CuO, Au, and TiO2 nanoparticles on daphnia magna and early life stages of zebrafish Danio rerio. Environ Prot Eng 40(1):139–149Google Scholar
- McCord JM, Fridovich I (1969) Superoxide dismutase, an enzymatic function for erythrocuprein (hemocurprein). J Biol Chem 244(22):6049–6055Google Scholar
- Memarizadeh N, Ghadamyari M, Adeli M, Talebi K (2014) Biochemical biomarkers of Glyphodes pyloalis Walker (Lepidoptera: Pyralidae) in exposure to TiO2 nanoparticles. Invertebr Surviv J 11:47–53Google Scholar
- Muramoto S (1983) Elimination of copper from Cu contaminated fish by long-term exposure to EDTA and freshwater. J Environ Sci Health 18(3):455–461Google Scholar
- Topal A, Alak G, Ozkaraca M, Cilingir Yeltekin A, Comaklı S, Acıl G, Kokturk M, Atamanalp M (2017) Neurotoxic responses in brain tissues of rainbow trout exposed to imidacloprid pesticide: assessment of 8-hydroxy-2-deoxyguanosine activity, oxidative stress and acetylcholinesterase activity. Chemosphere 175:186–191CrossRefGoogle Scholar