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Effect of Chronic Exposure to Methomyl on Tissue Damage and Apoptosis in Testis of Tilapia (Oreochromis niloticus) and Recovery Pattern

  • Shun Long Meng
  • Tao Liu
  • Xi Chen
  • Li Ping Qiu
  • Geng Dong Hu
  • Chao Song
  • LiMin Fan
  • Yao Zheng
  • Jia Zhang ChenEmail author
  • Pao XuEmail author
Article

Abstract

Tilapia were exposed to 0, 0.2, 2, 20, 200 µg/L methomyl for 30 days, and then transferred to methomyl-free water for 18 days. Caspase-8 in serum, apoptosis rate, microstructure and ultra-microstructure of testis were checked after methomyl exposure and at 18 days after transferring to methomyl-free water. There were no significant changes in Caspase-8 activity, apoptosis rate, and tissue structure in testis exposed to 0.2 and 2 µg/L compared with control. However, when tilapia exposed to 20 and 200 µg/L, the Caspase-8 activity and apoptosis rate were induced significantly, and tissue damage happened compared with the control. Thus it would appear 2 µg/L methomyl might be considered as the no observed adverse effect level. Recovery data showed that the effects produced by lower concentration of 20 µg/L were reversible but not at the higher 200 µg/L concentration.

Keywords

Methomyl Apoptosis rate Apoptosis enzyme Tissue structure Recovery pattern 

Notes

Acknowledgements

The authors acknowledge support from the Central Public-interest Scientific Institution Basal Research Fund, Freshwater Fisheries Research Center, CAFS (Grant No. 2017JBFM07) and China Agriculture Research System (Grant No. CARS-46) for the financial supports.

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Freshwater Fisheries Research CenterChinese Academy of Fishery SciencesWuxiPeople’s Republic of China
  2. 2.Wuxi Fishery CollegeNanjing Agricultural UniversityWuxiPeople’s Republic of China

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