Roundup® Herbicide Decreases Quality Parameters of Spermatozoa of Silversides Odontesthes Humensis
The silverside (Odontesthes humensis) is a very interesting model for toxicological studies due its high sensitivity and need for good water quality. The aim of this study was to evaluate the effects of Roundup on spermatozoa of O. humensis, after acute exposure. The fish were exposed to 0 and 7.8 mg L−1 (a.e.) of glyphosate, respectively. Through computer-assisted sperm analysis, a significant decrease in concentration, total and progressive motility, average path distance, straight line distance, path average velocity, curved line velocity, straight line velocity linearity, wobble, amplitude of lateral head displacement, cross beat frequency, and motility period of silverside spermatozoa exposed to Roundup was observed. Also, increase in membrane fluidity, ROS production and lipid peroxidation and a decrease in the mitochondrial functionality was observed in spermatozoa of Roundup exposed silversides. It was demonstrated that Roundup exposure in a concentration that can be achieve in natural water bodies soon after its application in fields is able to cause losses in several sperm quality parameters, consequently decreasing the fertilization potential of O. humensis spermatozoa.
KeywordsGlyphosate Sperm Fish Toxicity South America
This study was supported by the Brazilian CAPES, CNPQ (Grant No. 422292/2016-8) and FAPERGS (Grant No. 16/2551).
- Bemvenuti MA (2006) Silversides in South Brazil: morphological and ecological aspects. Biocell 30:111–118Google Scholar
- Christen R, Gatti J-L, Billard R (1987) Trout sperm motility: the transient movement of trout sperm is related to changes in the concentration of ATP following the activation of the flagellar movement. Eur J Biochem 166:667–671. https://doi.org/10.1111/j.1432-1033.1987.tb13565.x CrossRefGoogle Scholar
- Gillan L, Evans G, Maxwell WMC (2005) Flow cytometric evaluation of sperm parameters in relation to fertility potential. Theriogenology 63:445–457. https://doi.org/10.1016/j.theriogenology.2004.09.024 CrossRefGoogle Scholar
- Hulak M, Gazo I, Shaliutina A, Linhartova P (2013) In vitro effects of bisphenol A on the quality parameters, oxidative stress, DNA integrity and adenosine triphosphate content in sterlet (Acipenser ruthenus) spermatozoa. Comp Biochem Physiol C 158:64–71. https://doi.org/10.1016/j.cbpc.2013.05.002 Google Scholar
- Perchec G, Jeulin C, Cosson J et al (1995) Relationship between sperm ATP content and motility of carp spermatozoa. J Cell Sci 108:747–753Google Scholar
- Petrunkina AM, Volker G, Weitze K-F et al (2005) Detection of cooling-induced membrane changes in the response of boar sperm to capacitating conditions. Theriogenology 63:2278–2299. https://doi.org/10.1016/j.theriogenology.2004.10.008 CrossRefGoogle Scholar
- Pettitt MJ, Buhr MM (1998) Extender components and surfactants affect boar sperm function and membrane behaviour during cryopreservation. J Androl 19:736–746Google Scholar
- Salbego J, Pretto A, Gioda CR et al (2010) Herbicide formulation with glyphosate affects growth, acetylcholinesterase activity, and metabolic and hematological parameters in Piava (Leporinus obtusidens). Arch Environ Contam Toxicol 58:740–745. https://doi.org/10.1007/s00244-009-9464-y CrossRefGoogle Scholar
- Topal A, Atamanalp M, Uçar A et al (2015) Effects of glyphosate on juvenile rainbow trout (Oncorhynchus mykiss): transcriptional and enzymatic analyses of antioxidant defence system, histopathological liver damage and swimming performance. Ecotoxicol Environ Saf 111:206–214. https://doi.org/10.1016/j.ecoenv.2014.09.027 CrossRefGoogle Scholar
- Varela Junior AS, Corcini CD, Gheller SMM et al (2012) Use of amides as cryoprotectants in extenders for frozen sperm of tambaqui, Colossoma macropomum. Theriogenology 78:244–251. https://doi.org/10.1016/j.theriogenology.2012.02.029 CrossRefGoogle Scholar
- Zebral YD, Lansini LR, Costa PG et al (2018) A glyphosate-based herbicide reduces fertility, embryonic upper thermal tolerance and alters embryonic diapause of the threatened annual fish Austrolebias nigrofasciatus. Chemosphere 196:260–269. https://doi.org/10.1016/j.chemosphere.2017.12.196 CrossRefGoogle Scholar