Effect of EDTA and NTA on Arsenic Bioaccumulation and Translocation Using Phytoremediation by Mimosa pudica L. from Contaminated Soils

  • Pantawat Sampanpanish
  • Khamla Nanthavong


This study aimed to investigate the effects of Nitrilotriacetic acid (NTA) and Elthylenediaminetetraacetic acid (EDTA) on the bioaccumulation and translocation of arsenic (As) by Mimosa pudica L. using soils with 5 mg/kg of added As and NTA and EDTA concentrations of 50, 100, and 200 mg/kg. Soil and plant samples were collected every 30–120 days to analyze the As concentrations in the soil, underground part of the plants (root), and aboveground parts of the plants (shoots and leaves). The results showed that the plants with EDTA concentrations of 100 mg/kg had the highest As accumulation. At 120 days, M. pudica L. had a higher accumulation in the underground parts (29.71 mg/kg) than in the aboveground parts (6.32 mg/kg), with statistical significance (p < 0.05). The As translocation factor in the aboveground parts was less than 1, indicating As accumulation in the underground part only. With EDTA concentrations of 50 and 100 mg/kg, M. pudica L. had the highest bioaccumulation potential of As of 8.00 and 8.44, respectively. However, this research did not examine the reaction between As and any growth promoters. Further research should investigate the details of such a reaction at the molecular level, as well as explore how fertilizer factors might affect the As absorption of M. pudica L.


Phytoextraction Mimosa pudica L. Accumulation Chelating agent Arsenic 



The authors would like to thank the Office of Higher Education Commission (OHEC) and the S&T Postgraduate Education and Research Development Office (PERDO) for the financial support of the Research Program and the Ratchadaphiseksomphot Endowment Fund, Chulalongkorn University Research Unit. We also express our sincere thanks to the Environmental Research Institute, Chulalongkorn University (ERIC), the Center of Excellence on Hazardous Substance Management (HSM) and the Synchrotron Light Research Institute (SLRI) for their invaluable support in terms of facilities and scientific equipment.

Author’s Contributions

Authors participated in all experiments, coordinated the data-analysis and contributed to the written text of this manuscript.

Compliance with Ethical Standards

Conflict of interest

The authors declare no conflicts of interest.


  1. Aisien FA, Oboh IO, Aisien ET (2012) Phytotechnology remediation of inorganic contaminants. In: Anjum A, Pereira ME, Ahmad I, Duarte AC, Umar S, Khan NA (eds) Phytotechnologies. CRC Press, Boca Raton, pp 75–82. CrossRefGoogle Scholar
  2. Bu-Olayan AH, Thomas BV (2002) Bio-monitoring studies on the effect of lead in date palm (Phoenix dactylifera) in the arid ecosystem of Kuwait. J Arid Environ 51(1):133–139. CrossRefGoogle Scholar
  3. Chiu KK, Ye ZH, Wong MH (2005) Enhanced uptake of As, Zn, and Cu by Vetiveria zizanioides and Zea mays using chelating agents. Chemosphere 60(10):1365–1375. CrossRefGoogle Scholar
  4. Evangelou M, Ebel M, Schaeffer A (2007) Chelate assisted phytoextraction of heavy metals from soil. Effect, mechanism, toxicity and fate of chelating agents. Chemosphere 68(6):989–1003. CrossRefGoogle Scholar
  5. Goabas FAPC, Morrison HA (2000) Biococentration and biomagnification in the aquatic environment. In: Boethling RS, Mackay D (eds) Handbook of property estimation methods for chemicals: environmental and health sciences. Lewis, Boca Raton, pp 189–231Google Scholar
  6. Hsiao KH, Kao PH, Hseu ZY (2007) Effects of chelators on chromium and nickel uptake by Brassica juncea on serpentine-mine tailings for phytoextraction. J Hazard Mater 148(1–2):366–376. CrossRefGoogle Scholar
  7. Katagi T (2010) Bioconcentration, bioaccumulation, and metabolism of pesticides in aquatic organisms. Rev Environ Contam Toxicol.$41 Google Scholar
  8. Lu X, Kruatrachue M, Pokethitiyook P, Homyok K (2004) Removal of cadmium and zinc by water hyacinth Eichhornia crassipes. Sci Asia 30(10):93–103. CrossRefGoogle Scholar
  9. Otte ML, Dekkers IMJ, Rozema J, Broekman RA (1991) Uptake of by Aster tripolium in relation to rhizosphere oxidation. Can J Bot 69:2670–2677. CrossRefGoogle Scholar
  10. Pickering IJ, Prince RC, George MJ, Smith RD, George GN, Salt DE (2000) Reduction and coordination of in Indian mustard. Plant Physiol 122(4):1171–1177. CrossRefGoogle Scholar
  11. Sampanpanish P (2015) Phytoremediation, 1st edn. Chulalongkorn University, BangkokGoogle Scholar
  12. Schmoger MEV, Oven M, Grill E (2000) Detoxification of arsenic by phytochelatings in plants. Plant Physiol 122(3):793–801. CrossRefGoogle Scholar
  13. Smith E, Naidu R, Alston AM (2002) Arsenic in the soil environment. CRC for soil and land management glen Osmond, South Australia 5064 Australia. J Environ Qual 31:149–194. CrossRefGoogle Scholar
  14. Sun Y, Zhou Q, Wang L, Liu W (2009) The influence of different growth stages and dosage of EDTA on Cd uptake and accumulation in Cd-hyperaccumulator (Solanum nigrum L.). Bull Environ Contam Toxicol 82(3):348–353. CrossRefGoogle Scholar
  15. Surriya O, Sayeda S, Kinza W, Gul Kazi A (2015) Chap. 1-Phytoremediation of soils: prospects and challenges. Soil Rem Plants. Google Scholar
  16. Synchrotron Light Research Institute (2011) BL6b: micro-X-ray fluorescence and X-ray powder diffraction, Nakon Ratchasima Province, 250 km north-east of Bangkok, Thailand.
  17. Tananonchai A, Sampanpanish P (2014) Effect of EDTA and DTPA on cadmium removal from contaminated soil with water hyacinth. Appl Environ Res 36(3):65–76. Google Scholar
  18. USEPA (1996) Microwave assisted acid digestion of siliceous and organically based matrices. Method. 3052, Washington DCGoogle Scholar
  19. Wang KS, Huang LC, Lee HS, Chen PY, Chang SH (2008) Phytoextraction of cadmium by Ipomoea aquatica (water spinach) in hydroponic solution: effects of cadmium speciation. Chemosphere 72(4):666–672. CrossRefGoogle Scholar
  20. Yamamoto M, Yasuda M, Yamamoto Y (1985) Hydride-generation atomic absorption spectrometry coupled with flow injection analysis. Anal Chem 57(7):1382–1385. CrossRefGoogle Scholar
  21. Yang Y, Liu Z, Huang Y, Nan Z, Ma J, Wang H (2017) Effects of Cd on uptake of P in potato grown in sierozems. Soil Sedim Contam 26(3):323–335. CrossRefGoogle Scholar
  22. Zacchini M, Pietrini F, Mugnozza GS, Iori V, Pietrosanti L, Massacci A (2009) Metal tolerance, accumulation and translocation in poplar and willow clones treated with cadmium in hydroponics. Water Air Soil Pollut 197(1–4):23–34. CrossRefGoogle Scholar
  23. Zimmer D, Kruse J, Baum C, Borca C, Laue M, Hause G, Meissner R, Leinweber P (2011) Spatial distribution of arsenic and heavy metals in willow roots from a contaminated floodplain soil measured by X-ray fluorescence spectroscopy. Sci Total Environ 409:4094–4100. CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Environmental Research InstituteChulalongkorn University (ERIC)BangkokThailand
  2. 2.Research Program of Toxic Substance Management in the Mining IndustryCenter of Excellence on Hazardous Substance Management (HSM)BangkokThailand
  3. 3.Research Unit of Green Mining Management (GMM)Chulalongkorn UniversityBangkokThailand
  4. 4.International Postgraduate Program in Environmental Management, Graduate SchoolChulalongkorn UniversityBangkokThailand

Personalised recommendations