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Application of Toxigenic Alternaria oxytropis to Soybeans and its Effect on Swainsonine Detection in Different Environments

  • Odeshnee MoodleyEmail author
  • Yue Sun
  • Frederick Leo Sossah
  • Makoto Kakishima
  • Igor N. Pavlov
  • Yu Li
  • Qi WangEmail author
Article

Abstract

Alternaria oxytropis is an endophytic fungus of locoweeds that synthesizes swainsonine toxin. In this work, we evaluated the effect of A. oxytropis on soybean seedlings and quantified swainsonine in different culture conditions. Soybean (Glycine max) seeds were co-cultured with A. oxytropis (at different concentrations of mycelial suspensions) in agar media and soil culture, and swainsonine was assayed using LC–MS/MS. The results showed evidence that A. oxytropis infected soybean seedlings produced detectable swainsonine in agar culture while the toxin was undetectable or below the detection limit (0.006% of swainsonine dry weight) in soil media even at higher concentrations of the fungus. These results suggest that swainsonine detection is highly dependent on culture conditions and that soybeans co-cultured with A. oxytropis in soil could potentially be used to limit toxin production.

Keywords

Alkaloid toxin Alternaria oxytropis Soybean Swainsonine Locoweeds Toxin detection 

Notes

Acknowledgements

This work was funded by the Taishan Industry Leader Talent Project Fund (LJNY 201611) and the Program of Creation and Utilization of Germplasm of Mushroom Crop of “111 Plan” project (Grant No. D17014). We thank the following people for their contributions in this research study: Chunlan Zhang and Xiangyang Zhu.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Odeshnee Moodley
    • 1
    • 2
    Email author
  • Yue Sun
    • 1
  • Frederick Leo Sossah
    • 1
  • Makoto Kakishima
    • 1
    • 3
  • Igor N. Pavlov
    • 4
  • Yu Li
    • 1
  • Qi Wang
    • 1
    Email author
  1. 1.Engineering Research Centre of Chinese Ministry of Education for Edible and Medicinal FungiJilin Agricultural UniversityChangchunChina
  2. 2.South African Department of AgricultureForestry and Fisheries (DAFF)PretoriaSouth Africa
  3. 3.Life and Environmental SciencesUniversity of TsukubaTsukubaJapan
  4. 4.V.N. Sukachev Institute of Forest SB RASKrasnoyarskRussia

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