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Genome Analysis of Cytochrome in Dinotefuran-Treated Apolygus lucorum (Meyer-Dür)

  • Shaoying Wu
  • Denghui Deng
  • Wayne Jiang
  • Kun Zhang
  • Jianglong Guo
  • Wenbo Duan
  • Hao Wang
Article
  • 25 Downloads

Abstract

In this study, two CYP genes, CYP395G1 and CYP4EY1, were analyzed in Apolygus lucorum (Hemiptera: Miridae). The expression pattern in different developmental stages of both CYP395G1 and CYP4EY1 revealed that first instar nymphs possessed the highest gene-transcript levels. After 12 h of dinotefuran treatments, the expression levels of CYP395G1 increased by 1.92-fold, while the CYP4EY1 expression decreased. It was observed that the sensitivities of dinotefuran to laboratory strains from F0 to F9 and the mRNA expression levels of CYP395G1 in the F9 dinotefuran selected strain were higher when compared to the control strain. The results suggest that CYP395G1 was possibly a candidate P450 that was involved in dinotefuran detoxification. The dinotefuran resistant strain of A. lucorum was difficult to increase, due to its special molecular structure of dinotefuran compared with other neonicotinoids.

Keywords

Apolygus lucorum Cytochrome P450 monooxygenase Dinotefuran Induction Resistance 

Notes

Acknowledgements

This study was supported by National Natural Science Foundation of China: Construction of a suppression subtractive hybridization cDNA library and functional analysis of differentially expressed cytochrome P450s (Grant No. 31201541) and Chinese Postdoctoral Science Foundation (First Class Support) 2018M640675. The authors wish to thank the supports from Hainan University (HNU) and Michigan State University (MSU) and Henan Agricultural University (HAU).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.College of Plant ProtectionKey Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests (Hainan University), Ministry of EducationHaikouChina
  2. 2.Department of EntomologyMichigan State UniversityEast LansingUSA
  3. 3.College of Plant ProtectionHenan Agricultural UniversityZhengzhouChina

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