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Overexpression of Three Duplicated BnPCS Genes Enhanced Cd Accumulation and Translocation in Arabidopsis thaliana Mutant cad13

  • Jiuyuan Bai
  • Xin Wang
  • Rui Wang
  • Jing Wang
  • Sixiu Le
  • Yun ZhaoEmail author
Article

Abstract

Phytochelatins are widely known to chelate heavy metal in vacuole and decrease plant damage. Phytochelatin synthase gene (PCS), which is involved in phytochelatins synthesis, is commonly designated as a key gene for phytoremediation. In our study, we cloned three duplicated BnPCS genes from Brassica napus and transformed them into Arabidopsis thaliana AtPCS1 mutant cad13, respectively. Three transgene lines and cad1–3 were subjected to a cascade of concentrations of cadmium (Cd) treatment. Evaluation of morphological and physiological measurement results show that transgene lines possess higher Cd tolerance and resistance than A. thaliana mutant cad13. The analysis of PCs and Cd contents in root and shoot collectively indicated that transgenic plants promoted Cd accumulation and translocation. In conclusion, all the three BnPCS transgene lines enhanced Cd tolerance, accumulation and translocation, which could provide gene resources for phytoremediation.

Keywords

Duplicated genes Phytochelatin synthase Cadmium response Overexpression 

Notes

Acknowledgements

This work was supported by the Science and Technology Commission of Sichuan Province, the People’s Republic of China.

Supplementary material

128_2018_2487_MOESM1_ESM.docx (86 kb)
Supplementary material 1 (DOCX 85 KB)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Jiuyuan Bai
    • 1
  • Xin Wang
    • 1
  • Rui Wang
    • 1
  • Jing Wang
    • 1
  • Sixiu Le
    • 1
  • Yun Zhao
    • 1
    Email author
  1. 1.Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life SciencesSichuan UniversityChengduChina

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