Metallothionein Induced Time Dependent Histomorphometric Analysis of Clarias gariepinus Exposed to Cadmium
Cadmium (Cd), an extremely toxic heavy metal is extensively used in modern era because of its constructive chemical and physical properties. Recently Cd contamination was estimated in India’s major cities fresh water ecosystem, which may have firm impact on human health. Hence, this study was aimed to detect the time dependent effect of cadmium in fresh water fish C. gariepinus, a bioindicator species of water pollution. In a controlled environment, fishes were exposed to cadmium for different duration and analyzed for Cd accumulation. Cd induced toxicity was assessed by estimating metallothionein biomarker protein of heavy metal toxicity and histomorphometric changes in liver and kidney. Our results revealed that fish exposed to Cd induced apoptosis in fish tissues via induction of caspases and in contrast the metallothionein was also increased consistently with different doses of Cd exposure. Hence we conclude Cd induced structural damages to fishes are attributed to induction of caspases and estimating MT level in tissues can be effective biomarker to analyze the effect of acute environmental exposure to Cd.
KeywordsHeavy metal toxicity Cadmium Freshwater fish Histomorphometric analysis Metallothionein
The financial assistance and support from University Grants Commission (UGC), Government of India, New Delhi and Loyola College are greatly acknowledged.
- Bandara JM, Senevirathna DM, Dasanayake DM, Herath V, Bandara JM, Abeysekara T, Rajapaksha KH (2008) Chronic renal failure among farm families in cascade irrigation systems in Sri Lanka associated with elevated dietary cadmium levels in rice and freshwater fish (Tilapia). Environ Geochem Health 30(5):465–478CrossRefGoogle Scholar
- Environmental Protection Agency, EPA (2016) Aquatic Life Ambient Water Quality Criteria Cadmium (CAS # 7440-43-9). EPA 820-R-16-002, 30–87Google Scholar
- Food and Agriculture Organization of the United Nations, FAO (2016) Blue growth—unlocking the potential of seas and oceans. www.fao.org/zhc/details-events/en/c/233765/
- Ghiasi F, Mirzargar SS, Badakhshan H, Shamsi S (2010) Effects of low concentration of cadmium on the level of lysozyme in serum, leukocyte count and phagocytic index in Cyprinus carpio under the wintering conditions. JFAS 5:113–119Google Scholar
- Li Y, Chai X, Wu H, Jing W, Wang L (2013) The response of metallothionein and malondialdehyde after exclusive and combined Cd/Zn exposure in the crab Sinopotamon henanense. PLoS ONE 8(11) e80475Google Scholar
- Prakash S, Prithiviraj E, Suresh S (2008) Developmental changes of seminiferous tubule in prenatal, postnatal and adult testis of bonnet monkey (Macaca radiata). Anat Histol Embryol 37(1):19–23Google Scholar
- Rico D, Martín-González A, Díaz S, de Lucas P, Gutiérrez JC (2009) Heavy metals generate reactive oxygen species in terrestrial and aquatic ciliated protozoa. Comp Biochem Physiol C 149(1):90–96Google Scholar
- Rose S, Vincent S, Meena B, Suresh A, Mani R (2014) Immunolocalization of metallothionein in different tissues of fresh water catfish, C. gariepinus on exposure to cadmium. IJPSR 5(12):1000–1006Google Scholar
- Underwood W, Anthony R, Gwaltney-Brant S, Poison ASPCA, Meyer R (eds)(2013) AVMA guidelines for the euthanasia of animals. Schaumburg, American Veterinary Medical AssociationGoogle Scholar
- Wang WX, Rainbow PS (2010) Significance of metallothioneins in metal accumulation kinetics in marine animals. Comp Biochem Physiol C 2(1):1–8Google Scholar
- Zorita I, Strogyloudi E, Buxens A, Mazón LI, Papathanassiou E (2005) Application of two SH-based methods for metallothionein determination in mussels and intercalibration of the spectrophotometric method: laboratory and field studies in the Mediterranean Sea. Biomarkers 10(5):342–359CrossRefGoogle Scholar