Advertisement

Sperm Morphology and Motility of Bufo raddei Under Long-Term Environmental Heavy Metal Stress

  • Rui Guo
  • Wenya ZhangEmail author
  • Ying Yang
  • Jian Ding
  • Shiwei Ai
  • Wenzhi Yang
  • Xiaojuan Bai
  • Yingmei ZhangEmail author
Article

Abstract

The present study aimed to investigate the effects of environmental heavy metals on sperm morphology and motility of Bufo raddei. We collected B. raddei from Baiyin (BY) and Liujiaxia (LJX); BY is an area heavily polluted by heavy metals, while LJX is a relatively unpolluted area. Significant accumulations of copper, zinc, lead and cadmium were observed in B. raddei from BY, together with relatively smaller sperm size and higher sperm abnormality rate. Unexpectedly, sperm velocity, percentages of sperm motility and viability of B. raddei from BY were significantly higher than those from LJX. Similarly, when sperm was suspended in heavy metal solutions, sperm velocity, percentages of sperm motility and viability significantly increased compared with those suspended in distilled water. These results indicate that environmental heavy metal stress may decrease sperm size, meanwhile enhance sperm motility through increasing the ratio of head length to total sperm length of B. raddei.

Keywords

Heavy metal Bufo raddei Sperm morphology Sperm motility 

Notes

Acknowledgements

The present study was supported by the National Natural Science Foundation of China (No: 31300437) and the Fundamental Research Funds for the Central Universities (Nos: lzujbky-2018-kb18 and lzujbky-2017-it49).

Compliance with Ethical Standards

Ethical Approval

Animal studies and experiments were approved and carried out according to Lanzhou University’s Institutional Animal Care and Use Committee guidelines.

Supplementary material

128_2018_2408_MOESM1_ESM.docx (17 kb)
Supplementary material 1 (DOCX 17 KB)

References

  1. Akinloye O, Abbiyesuku FM, Oguntibeju OO, Arowojolu AO, Truter EJ (2011) The impact of blood and seminal plasma zinc and copper concentrations on spermogram and hormonal changes in infertile Nigerian men. Reprod Biol 11:83–98CrossRefGoogle Scholar
  2. Boran C, Ozkan KU (2004) The effect of zinc therapy on damaged testis in pre-pubertal rats. Pediatr Surg Int 20:444–448CrossRefGoogle Scholar
  3. Cabrera-Guzman E, Crossland MR, Brown GP, Shine R (2013) Larger body size at metamorphosis enhances survival, growth and performance of young cane toads (Rhinella marina). PLoS ONE 8:70121CrossRefGoogle Scholar
  4. Canli M, Atli G (2003) The relationships between heavy metal (Cd, Cr, Cu, Fe, Pb, Zn) levels and the size of six Mediterranean fish species. Environ Pollut 121:129–136CrossRefGoogle Scholar
  5. Della TG, Trudeau VL, Gratwicke B, Evans M, Augustine L, Chia H, Bronikowski EJ, Murphy JB, Comizzoli P (2017) Effects of hormonal stimulation on the concentration and quality of excreted spermatozoa in the critically endangered Panamanian golden frog (Atelopus zeteki). Theriogenology 91:27–35CrossRefGoogle Scholar
  6. Dietrich GJ, Dietrich M, Kowalski RK, Dobosz S, Karol H, Demianowicz W, Glogowski J (2010) Exposure of rainbow trout milt to mercury and cadmium alters sperm motility parameters and reproductive success. Aquat Toxicol 97:277–284CrossRefGoogle Scholar
  7. Dziminski MA, Roberts JD, Simmons LW (2010) Sperm morphology, motility and fertilisation capacity in the myobatrachid frog Crinia georgiana. Reprod Fertil Dev 22:516–522CrossRefGoogle Scholar
  8. Gage MJG, Macfarlane CP, Yeates S, Ward RG, Searle JB, Parker GA (2004) Spermatozoal traits and sperm competition in Atlantic salmon: relative sperm velocity is the primary determinant of fertilization success. Curr Biol 14:44–47Google Scholar
  9. Guo R, Zhang WY, Ai SW, Ren L, Zhang YM (2017) Fluctuating asymmetry rather than oxidative stress in Bufo raddei can be an accurate indicator of environmental pollution induced by heavy metals. Environ Monit Assess 189:293CrossRefGoogle Scholar
  10. Hemelaar A (1985) An improved method to estimate the number of year rings resorbed in phalanges of Bufo bufo (L.) and its application to populations from different latitudes and altitudes. Amphibia-Reptilia 6:323–341CrossRefGoogle Scholar
  11. Karraker NE, Welsh HH Jr (2006) Long-term impacts of even-aged timber management on abundance and body condition of terrestrial amphibians in Northwestern California. Biol Conserv 131:132–140CrossRefGoogle Scholar
  12. Krapf D, O’Brien ED, Cabada MO, Visconti PE, Arranz SE (2009) Egg water from the amphibian Bufo arenarum modulates the ability of homologous sperm to undergo the acrosome reaction in the presence of the vitelline envelope. Biol Reprod 80:311–319CrossRefGoogle Scholar
  13. Kumosani TA, Elshal MF, Al-Jonaid AA, Abduljabar HS (2008) The influence of smoking on semen quality, seminal microelements and Ca2+-ATPase activity among infertile and fertile men. Clin Biochem 41:1199–1203CrossRefGoogle Scholar
  14. Kuwahara M, Gu Y, Ishibashi K, Marumo F, Sasaki S (1997) Mercury-sensitive residues and pore site in AQP3 water channel. Biochemistry 36:13973–13978CrossRefGoogle Scholar
  15. Lahnsteiner F, Berger B, Weismann T (1999) Sperm metabolism of the telost fishes Chalcalburnus chalcoides and Oncorhynchus mykiss and its relation to motility and viability. J Exp Zool 284:454–465CrossRefGoogle Scholar
  16. Liu BL, Ma XW, Ai SW, Zhu SY, Zhang WY, Zhang YM (2016) Spatial distribution and source identification of heavy metals in soils under different land uses in a sewage irrigation region, northwest China. J Soil Sediment 16:1547–1556CrossRefGoogle Scholar
  17. Lupold S, Calhim S, Immler S, Birkhead TR (2009) Sperm morphology and sperm velocity in passerine birds. Proc Biol Sci 276:1175–1181CrossRefGoogle Scholar
  18. Martelli A, Rousselet E, Dycke C, Bouron A, Moulis JM (2006) Cadmium toxicity in animal cells by interference with essential metals. Biochimie 88:1807–1814CrossRefGoogle Scholar
  19. Meeker JD, Rossano MG, Protas B, Diamond MP, Puscheck E, Daly D, Paneth N, Wirth JJ (2008) Cadmium, lead, and other metals in relation to semen quality: human evidence for molybdenum as a male reproductive toxicant. Environ Health Perspect 116:1473–1479CrossRefGoogle Scholar
  20. Nystrom P, Hansson J, Mansson J, Sundstedt M, Reslow C, Brostrom A (2007) A documented amphibian decline over 40 years: possible causes and implications for species recovery. Biol Conserv 138:399–411CrossRefGoogle Scholar
  21. Qureshi IZ, Kashif Z, Hashmi MZ, Su XM, Malik RN, Ullah K, Hu JX, Dawood M (2015) Assessment of heavy metals and metalloids in tissues of two frog species: Rana tigrina and Euphlyctis cyanophlyctis from industrial city Sialkot, Pakistan. Environ Sci Pollut Res 22:14157–14168CrossRefGoogle Scholar
  22. Rao MV, Gangadharan B (2008) Antioxidative potential of melatonin against mercury induced intoxication in spermatozoa in vitro. Toxicol In Vitro 22:935–942CrossRefGoogle Scholar
  23. Sharma B, Patino R (2009) Effects of cadmium on growth, metamorphosis and gonadal sex differentiation in tadpoles of the African clawed frog, Xenopus laevis. Chemosphere 76:1048–1055CrossRefGoogle Scholar
  24. Snook RR (2005) Sperm in competition: not playing by the numbers. Trends Ecol Evol 20:46–53CrossRefGoogle Scholar
  25. Sun N, Wang H, Ju Z, Zhao H (2018) Effects of chronic cadmium exposure on metamorphosis, skeletal development, and thyroid endocrine disruption in Chinese toad Bufo gargarizans tadpoles. Environ Toxicol Chem 37:213–223CrossRefGoogle Scholar
  26. Tvrda E, Peer R, Sikka SC, Agarwal A (2015) Iron and copper in male reproduction: a double-edged sword. J Assist Reprod Genet 32:3–16CrossRefGoogle Scholar
  27. Xu LC, Wang SY, Yang XF, Wang XR (2001) Effects of cadmium on rat sperm motility evaluated with computer assisted sperm analysis. Biomed Environ Sci 14:312–317Google Scholar
  28. Yamaguchi S, Miura C, Kikuchi K, Celino FT, Agusa T, Tanabe S, Miura T (2009) Zinc is an essential trace element for spermatogenesis. Proc Natl Acad Sci USA 106:10859–10864CrossRefGoogle Scholar
  29. Zhang HJ, Cai CC, Shi CL, Cao H, Han ZL, Jia XY (2012) Cadmium-induced oxidative stress and apoptosis in the testes of frog Rana limnocharis. Aquat Toxicol 122–123:67–74CrossRefGoogle Scholar
  30. Zhang WY, Guo R, Ai SW, Yang Y, Ding J, Zhang YM (2018) Long-term heavy metal pollution varied female reproduction investment in free-living anura, Bufo raddei. Ecotoxicol Environ Saf 159:136–142CrossRefGoogle Scholar
  31. Zhou QF, Zhang JB, Fu JJ, Shi JB, Jiang GB (2008) Biomonitoring: an appealing tool for assessment of metal pollution in the aquatic ecosystem. Anal Chim Acta 606:135–150CrossRefGoogle Scholar
  32. Zocche JJ, Damiani AP, Hainzenreder G, Mendonc RA, Peres PB, dos Santosc CEI, Debastiani R, Diasc JF, Andrade VM (2013) Assessment of heavy metal content and DNA damage in Hypsiboas faber (anuran amphibian) in coal open-casting mine. Environ Toxicol Pharmacol 36:194–201CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Gansu Key Laboratory of Biomonitoring and Bioremediation for Environmental Pollution, School of Life SciencesLanzhou UniversityLanzhouPeople’s Republic of China

Personalised recommendations