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Diversity of the Aromatic-Ring-Hydroxylating Dioxygenases in the Monoaromatic Hydrocarbon Degraders Held by a Common Ancestor

Abstract

Aromatic ring hydroxylating dioxygenases (ARHDs), harboured by a variety of bacteria, catalyze the initial reaction in the degradation of a wide range of toxic environmental contaminants like aromatic and polycyclic aromatic hydrocarbons (PAHs). Regardless of the source, bacteria harbouring RHDs play major role in the removal of these toxic contaminants. The diversity of ARHDs in contaminated sites is supposed to be huge. However, most of the ARHD diversity studies are based on the PAH degraders and the ARHD diversity in the monoaromatic hydrocarbon degraders has not fully explored yet. In this study, therefore, the ARHD gene from nine different genara of the monoaromatic hydrocarbon degraders including Raoultella, Stenotrophomons, Staphylococcus, Acinetobacter, Pseudomonas, Serratia, Comamonas, Pantoea, and Micrococcus was analysed through polymerase chain reactions and sequencing. The sequence alignments of the ARHD amplicons with 81%–99% homologies were found to be highly related and held by divergent evolution from a common ancestor.

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Acknowledgements

The authors would like to thank Middle East Technical University Research Fund for the financial support through the projects BAP-03-11-2016-001.

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Correspondence to Bulent Icgen.

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Yavas, A., Icgen, B. Diversity of the Aromatic-Ring-Hydroxylating Dioxygenases in the Monoaromatic Hydrocarbon Degraders Held by a Common Ancestor. Bull Environ Contam Toxicol 101, 410–416 (2018). https://doi.org/10.1007/s00128-018-2350-4

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Keywords

  • ARHD
  • Aromatic ring hydroxylating dioxygenases
  • TodC1
  • Hydrocarbon degraders