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Partial mRNA Sequences of the Biomarkers CYP1A, GST, CAT, GR, SOD, GPx, VTG and p53 in Flatfish Syacium gunteri from Gulf of Mexico

Abstract

The present study reports partial sequences of a group of genes used as exposure and effect biomarkers of organic contaminants and/or heavy metals in Syacium gunteri. In order to isolate these sequences, cDNA was used to amplify fragments between 200 and 800 bp, which were then cloned and sequenced. The sequences presented high percentages of identity with genes involved in the metabolism of xenobiotic biotransformation (cytochrome P4501A and glutathione S-transferase), oxidative stress (catalase, glutathione reductase, glutathione peroxidase and superoxide dismutase), reproductive system (vitellogenin) and with the tumor suppressor protein p53 reported in the GenBank database. Subsequently, from the deduced sequence of amino acids of each fragment, their tridimensional structure was predicted, using homologous proteins from the Protein Data Base. This study generates an important base of molecular biomarkers for the monitoring of environmental health in the Gulf of Mexico.

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Acknowledgements

We thank PEMEX Exploracion y Produccion (PEP), for the financial support granted during the environmental monitoring services of the Gulf of Mexico carried out by the CINVESTAV-IPN Campus Merida and the Sector Fund CONACyT - SENER - Hydrocarbons, project 201441. This is a contribution from the Gulf of Mexico Research Consortium (CIGoM).

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Correspondence to Omar Zapata-Pérez.

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Puch-Hau, C., Quintanilla-Mena, M., Rubio-Piña, J. et al. Partial mRNA Sequences of the Biomarkers CYP1A, GST, CAT, GR, SOD, GPx, VTG and p53 in Flatfish Syacium gunteri from Gulf of Mexico. Bull Environ Contam Toxicol 100, 798–802 (2018). https://doi.org/10.1007/s00128-018-2329-1

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Keywords

  • Molecular biomarkers
  • Contaminants
  • Environmental monitoring
  • Syacium gunteri