Effects of Dracontomelon duperreanum Leaf Litter on the Growth and Photosynthesis of Microcystis aeruginosa

  • Xiaoxiong Wang
  • Yim Tong Szeto
  • Chengchun Jiang
  • Xiaojun Wang
  • Yi Tao
  • Jianguo Tu
  • Jing Chen
Article

Abstract

This study investigated the use of Dracontomelon duperreanum leaf litter extract (DDLLE) in inhibiting the growth and photosynthesis of the algae Microcystis aeruginosa. The goal of the study was to evaluate a potential solution for cyanobacterial bloom prevention. M. aeruginosa was exposed to extract concentrations from 0.4 to 2.0 g L−1. Chlorophyll-a (Chl-a) content and photosynthesis levels were assessed using pulse amplitude modulated fluorimetry phytoplankton analyzer. Results suggested that the extract could efficiently suppress M. aeruginosa growth. The content of Chl-a was only 19.0 µg L−1 and achieved 96.0% inhibition rate when exposed to 2.0 g L−1 on day 15. Growth rate in response to different extract concentrations were consistent with changes in the photosynthesis efficiency (alpha), maximal relative electron transport rate and maximal photochemical efficiency of photosystem II (F v /F m ). Furthermore, several kinds of volatile chemicals and their concentrations in DDLLE had been identified by GC–MS, which of them play major role to suppress the growth of M. aeruginosa should be further studied.

Keywords

Allelopathy Dracontomelon duperreanum Growth suppression Microcystis aeruginosa Phyto-PAM 

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation (NSFC) of China, Grant No. 5137836 and the Science, Industry, Trade and Information Technology Commission of Shenzhen Municipality (JCYJ20150430163657307) for their financial support.

References

  1. Daniel ET, Ferrier MD, Armbrester EA, Anlauf KA (2002) Inhibition of dinoflagellate growth by extracts of barley straw (Hordeum vulgare). J Appl Phycol 14:275–280CrossRefGoogle Scholar
  2. Ferrier MD, Butler BR, Terlizzi DE, Lacouture R (2005) The effects of barley straw (Hordeum vulgare) on the growth of freshwater algae. Bioresour Technol 96:1788–1795CrossRefGoogle Scholar
  3. Gao L, Xie L (2011) Analysis of the influence of meteorological condition on cyanobacterial bloom and treatment methods in Taihu Lake. China Resour Compr Utilization 29:35–38 (In Chinese)Google Scholar
  4. Hong Y, Hu HY, Sakoda A, Sagehashi M (2011) Straw preservation effects of Arundo donax L. on its allelopathic activity to toxic and bloom-forming Microcystis aeruginosa. Water Sci Technol 63:1566–1573CrossRefGoogle Scholar
  5. Huang HM, Xiao X, Ghadouani A, Wu JP, Nie Z, Peng YC, Xu XH, Shi JY (2015) Effects of natural flavonoids on photosynthetic activity and cell integrity in Microcystis aeruginosa. Toxins 7:66–80CrossRefGoogle Scholar
  6. Jančula D, Maršálek B (2011) Critical review of actually available chemical compounds for prevention and management of cyanobacterial blooms. Chemosphere 85:1415–1422CrossRefGoogle Scholar
  7. Jia CS (2006) Calculatiang the LC50 of insecticides with software SPSS. Chin Bull Entomol 43:414–417 (Chinese)Google Scholar
  8. Li FM, Hu HY (2005) Isolation and characterization of a novel anti-algal allelochemical from Phragmites communis. Appl Environ Microbiol 71:6545–6553CrossRefGoogle Scholar
  9. Lu ZY, Sha J, Tian Y, Zhang XZ, Liu BY, Wu ZB (2017) Polyphenolic allelochemical pyrogallic acid induces caspase-3 (like)-dependent programmed cell death in the cyanobacterium Microcystis aeruginosa. Algal Res 21:148–155CrossRefGoogle Scholar
  10. Macías FA, Oliva RM, Simonet AM, Galindo JCG (1998) What are allelochemicals? In: Olofsdotter M (ed) Allelopathy in Rice. IRRI Press, ManillaGoogle Scholar
  11. Nakai S, Yamad S, Hosomi M (2005) Anti-cyanobacterial fatty acids released from Myriophyllum spicatum. Hydrobiologia 543:71–78CrossRefGoogle Scholar
  12. Paeri HW, Huisman J (2008) Climate-blooms like it hot. Science 320:57–58CrossRefGoogle Scholar
  13. Pan Q, Zou GY, Song XF, Nakai SS, Fu ZS (2014) Inhibitory effects of the roots of floating bed plants of Canna indica on Microcystis aeruginosa. Res of Environ Sci 27:193–1198 (Chinese)Google Scholar
  14. Platt T, Gallegos CL, Harrison WG (1980) Photoinhibition of photosynthesis in natural assemblages of marine phytoplankton. J Mar Res 38:687–701Google Scholar
  15. Qian HF, Xu XY, Chen W, Jiang H, Jin YX, Liu WP, Fu ZW (2009) Allelochemical stress causes oxidative damage and inhibition of photosynthesis in Chlorella vulgaris. Chemosphere 75:368–375CrossRefGoogle Scholar
  16. Ralph PJ, Gademann R (2005) Rapid light curves: a powerful tool to assess photosynthetic activity. Aquat Bot 82:222–237CrossRefGoogle Scholar
  17. Rice EL (1984) Allelopathy, 2nd edn. Academic Press, OrlandoGoogle Scholar
  18. Ridge I, Pillinger JM (1996) Towards understanding the nature of algal inhibitors from barley straw. Hydrobiologia 120:301–305CrossRefGoogle Scholar
  19. Ridge I, Walters J, Street M (1999) Algal growth control by terrestrial leaf litter: a realistic tool? Hydrobiologia 395–396:173–180CrossRefGoogle Scholar
  20. Rippka R, Deruelles J, Waterbury JB, Herdman M, Stanier RY (1979) Genetic assignments, stain histories and properties of pure culture of cyanobacteria. J Gen Microbiol 111:1–61Google Scholar
  21. Schrader KK, de Regt MQ, Tidwell PR, Turker CS, Duke SO (1998) Selective growth inhibition of the mushy-odour producing cyanobacterium Oscillatria cf. chalybea by natural compounds. Bull Environ Contam Toxicol 60:651–658CrossRefGoogle Scholar
  22. Su XF, Nong WT (2010) Study on antimicrobial activity of extracts from Dracontomelon duperreanum. Chin Pharm 21:2115–2117 (Chinese)Google Scholar
  23. Su W, Johannes AH, Jia YH, Lu YP, Kong FX (2014) Effects of rice straw on the cell viability, photosynthesis, and growth of Microcystis aeruginosa. Chin J Oceanol Limn 32:120–129CrossRefGoogle Scholar
  24. Suggett DJ, Borowitzka MA, Prášil O (2010) Chlorophyll a fluorescence in aquatic sciences: methods and applications. Springer, LondonCrossRefGoogle Scholar
  25. Wang R, Hua M, Yu Y, Zhang M, Xian QM, Yin DQ (2016a) Evaluating the effects of allelochemical ferulic acid on Microcystis aeruginosa by pulse-amplitude-modulated (PAM) fluorometry and flow cytometry. Chemosphere 147:264–271CrossRefGoogle Scholar
  26. Wang XX, Jiang CC, Szeto YT, Li HK, Yam KL, Wang XJ (2016b) Effects of Dracontomelon duperreanum defoliation extract on Microcystis aeruginosa: physiological and morphological aspects. Environ Sci Pollut Res 23:8731–8740CrossRefGoogle Scholar
  27. Wang SB, Wang YN, Ma XX, Xu ZR (2016c) Effects of garlic and diallyl trisulfide on the growth, photosynthesis, and alkaline phosphatase activity of the toxic cyanobacterium Microcystis aeruginosa. Environ Sci Pollut Res 23:5712–5720CrossRefGoogle Scholar
  28. Xiao X, Chen YX, Liang XQ, Lou LP, Tang XJ (2010) Tibetan hulless barley efficiently inhibited bloom-forming cyanobacterium Microcystis aeruginosa. Chemosphere 81:1118–1123CrossRefGoogle Scholar
  29. Zhou SQ, Shao YS, Gao NY, Deng Y, Qiao JL, Ou HS, Deng J (2013) Effects of different algaecides on the photosynthetic capacity, cell integrity and microcystin-LR release of Microcystis aeruginosa. Sci Total Environ 463–464:111–119CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Xiaoxiong Wang
    • 1
    • 2
  • Yim Tong Szeto
    • 3
  • Chengchun Jiang
    • 2
  • Xiaojun Wang
    • 1
  • Yi Tao
    • 4
  • Jianguo Tu
    • 5
  • Jing Chen
    • 5
  1. 1.College of Environment and EnergySouth China University of TechnologyGuangzhouChina
  2. 2.School of Civil and Environmental EngineeringShenzhen PolytechnicShenzhenChina
  3. 3.Diagnostix Medical CentreCentral, Hong KongChina
  4. 4.Key Laboratory of Microorganism Application and Risk Control (MARC) of Shenzhen, Graduate School at ShenzhenTsinghua UniversityShenzhenChina
  5. 5.Shenzhen Center for Analysis and Measurement of Material SurfaceShenzhenChina

Personalised recommendations