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Identification of Sesbania sesban (L.) Merr. as an Efficient and Well Adapted Phytoremediation Tool for Cd Polluted Soils

  • Mayank Varun
  • Clement O. Ogunkunle
  • Rohan D’Souza
  • Paulo Favas
  • Manoj Paul
Article

Abstract

A pot experiment was carried out to assess Cd uptake and accumulation efficiency of Sesbania sesban. Plants were grown in soil spiked with 25, 50, 100, 150, 200, 250, and 300 mg/kg Cd. After 120 days, plants were harvested and analyzed for Cd content. A steady increase in Cd accumulation with increasing metal concentration in soil was observed for all treatments. Accumulation of Cd was greatest in roots (86.7 ± 6.3 mg/kg), followed by stem (18.59 ± 1.9 mg/kg), and leaf (3.16 ± 1.1 mg/kg). Chlorophyll content declined with increasing Cd concentration, while proline and protein content increased as compared to control. At higher Cd levels, root, shoot length, and biomass were all significantly reduced (p ≤ 0.001). An increase in total protein along with greater A250/A280 value suggested an increase in metal-protein complexes. Considering the rapid growth, high biomass, accumulation efficiency, and adaptive properties, this plant could be used as a valuable tool for the phytoremediation of Cd contaminated soils.

Keywords

Toxicity Soil contamination Metal Phytoextraction 

Notes

Acknowledgements

We gratefully acknowledge the University Grants Commission for providing financial support by sanctioning the Post Doctoral Fellowship No. F./PDFSS-2014-15-SC-UTT-8854 to Mayank Varun. The support of Fundação para a Ciência e a Tecnologia, through the strategic project UID/MAR/04292/2013 granted to MARE is gratefully acknowledged.

References

  1. Abbas SZ, Rafatullah M, Ismail N, Lalung J (2014) Isolation, identification, characterization, and evaluation of cadmium removal capacity of Enterobacter species. J Basic Microbiol 54:1279–1287CrossRefGoogle Scholar
  2. Adriano D (2001) Cadmium. In: Trace elements in terrestrial environments: biogeochemistry, bioavailability, and risks of metals, 2nd edn. Springer-Verlag, New York, pp. 264–314CrossRefGoogle Scholar
  3. Aery NC, Rana DK (2003) Growth and cadmium uptake in barley under cadmium stress. J Environ Biol 24:117–123Google Scholar
  4. Ahmed I, Akhtar MJ, Zahir ZA, Jamil A (2012) Effect of cadmium on seed germination and seedling growth of four wheat (Triticum aestivum L.) cultivars. Pak J Bot 44(5):1569–1574Google Scholar
  5. Arnon DI (1949) Copper enzymes in isolated chloroplasts: polyphenol oxidase in Beta vulgaris. Plant Physiol 24:1–15CrossRefGoogle Scholar
  6. Ashraf M, Foolad MR (2007) Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environ Exp Bot 59:206–216CrossRefGoogle Scholar
  7. Baryla A, Carrier P, Franck F, Coulomb C, Sahut C, Havaux M (2001) Leaf chlorosis in oilseed rape plants (Brassica napus) grown on cadmium-polluted soil: causes and consequences for photosynthesis and growth. Planta 212:696–709CrossRefGoogle Scholar
  8. Bates LS, Waldren RD, Teare TD (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–207CrossRefGoogle Scholar
  9. Bhargava A, Shukla S, Srivastava J, Singh N, Ohri D (2008) Chenopodium: a prospective plant for phytoextraction. Acta Physiol Plant 30:111–120CrossRefGoogle Scholar
  10. Branzini A, González RS, Zubillaga M (2012) Absorption and translocation of copper, zinc and chromium by Sesbania virgate. J Environ Manage 102:50–54CrossRefGoogle Scholar
  11. Chen X, Wang J, Shi Y, Zhao MQ, Chi GY (2011) Effects of cadmium on growth and photosynthetic activities in pakchoi and mustard. Bot Stud 52:41–46Google Scholar
  12. D’Souza R, Varun M, Masih J, Paul MS (2010) Identification of Calotropis procera L. as a potential phytoaccumulator of heavy metals from contaminated soils in urban north central India. J Haz Mat 184:457–464CrossRefGoogle Scholar
  13. D’Souza R, Varun M, Pratas J, Paul MS (2013) Spatial distribution of heavy metals in soil and flora associated with the glass industry in North Central India: implications for phytoremediation. Soil Sediment Contam 22:1–20CrossRefGoogle Scholar
  14. Degefu T, Wolde-Meskel E, Frostegard A (2011) Multilocus sequence analyses reveal several unnamed Mesorhizobium genospecies nodulating Acacia species and Sesbania sesban trees in southern regions of Ethiopia. Syst Appl Microbiol 34:216–226CrossRefGoogle Scholar
  15. Dias LE, Melo RF, de Mello JWV, Oliveira JA, Daniels WL (2010) Growth of seedlings of pigeon pea (Cajanus cajan (L.) Millsp), and riverhemp (Sesbania virgata (Cav.) Pers.), and lead tree (Leucaena leucocephala (Lam.) De Wit) in an arsenic-contaminated soil. R Bras Ci Solo 34:975–983CrossRefGoogle Scholar
  16. Dinakar N, Nagajyothi PC, Suresh S, Damodharam T, Suresh C (2009) Cadmium induced changes on proline, antioxidant enzymes, and nitrite reductases in Arachis hypogea L. J Environ Biol 30(2):289–294Google Scholar
  17. Faheed FA (2005) Effect of lead stress on the growth and metabolism of Eruca sativa M. seedlings. Acta Agronom Hung 53(3):319–327CrossRefGoogle Scholar
  18. Favas PJC, Pratas J, Varun M, D’Souza R, Paul MS (2014) Accumulation of uranium by aquatic plants in field conditions: potential for phytotechnologies. Sci Total Environ 470–471:993–1002CrossRefGoogle Scholar
  19. Figueiredo NL, Areias A, Mendes R, Canário J, Duarte A, Carvalho C (2014) Mercury-resistant bacteria from salt marsh of Tagus Estuary: the influence of plants presence and mercury contamination levels. J Toxicol Environ Health A 77:959–971CrossRefGoogle Scholar
  20. Fitz WJ, Wenzel WW (2002) Arsenic transformation in the soil-rhizosphere-plant system, fundamentals and potential application of phytoremediation. J Biotechnol 99:259–278CrossRefGoogle Scholar
  21. Ghosh M, Singh SP (2005) A review on phytoremediation of heavy metals and utilization of its byproducts. Appl Ecol Environ Res 3(1):1–18CrossRefGoogle Scholar
  22. Ginneken LV, Meers E, Guisson R, Ruttens A, Elst K, Tack FMG, Vangronsveld J, Diels L, Dejonghe W (2007) Phytoremediation for heavy metal contaminated soils combined with energy production. J Environ Eng Landsc Manage 15:227–236Google Scholar
  23. Hirve M, Bafna A (2013) Effect of cadmium exposures on growth and biochemical parameters of Vigna radiata seedlings. Int J Environ Sci 4:315–322Google Scholar
  24. Khatamipour M, Piri E, Esmaeilian Y, Tavassoli A (2011) Toxic effect of cadmium on germination, seedling growth and proline content of milk thistle (Silybum marianum). Ann Biol Res 2(5):527–532Google Scholar
  25. Kim S, Takahashi M, Higuchi K, Tsunoda K, Nakanishi H, Yoshimura E, Mori S, Nishizawa N (2005) Increased nicotianamine biosynthesis confers enhanced tolerance of high levels of metals, in particular nickel, to plants. Plant Cell Physiol 46(11):1809–1818CrossRefGoogle Scholar
  26. Küpper H, Lombi E, Zhao FJ, McGrath SP (2000) Cellular compartmentation of cadmium and zinc in relation to other elements in the hyperaccumulator Arabidopsis halleri. Planta 212:75–84CrossRefGoogle Scholar
  27. Lowry OH, Rosebrough NJ, Farr AL, Randall RL (1951) Protein measurement with the folin reagent. J Biol Chem 193:265–275Google Scholar
  28. Mahmood T (2010) Phytoextraction of heavy metals: the process and scope for remediation of contaminated soils. Soil Environ 29:91–109Google Scholar
  29. Malar S, Manikandan R, Favas PJC, Sahi SV, Venkatachalam P (2014) Effect of lead on phytotoxicity, growth, biochemical alterations and its role on genomic template stability in Sesbania grandiflora: a potential plant for phytoremediation. Ecotoxicol Environ Saf 108:249–257CrossRefGoogle Scholar
  30. Malar S, Sahi SV, Favas PJC, Venkatachalam P (2015) Assessment of mercury heavy metal toxicity-induced physiochemical and molecular changes in Sesbania grandiflora L. Int J Environ Sci Technol 12(10):3273–3282CrossRefGoogle Scholar
  31. Mangal M, Agarwal M, Bhaegava D (2013) Effect of cadmium and zinc on growth and biochemical parameters of selected vegetables. J Pharmacogn Phytochem 2(1):106–114Google Scholar
  32. McComb J, Hentz S, Miller GS, Begonia M, Begonia G (2012) Effects of lead on plant growth, lead accumulation and phytochelatin contents of hydroponically-grown Sesbania exaltata. World Environ 2(3):38–43CrossRefGoogle Scholar
  33. McGrath SP, Zhao FJ, Lombi E (2001) Plant and rhizosphere processes involved in phytoremediation of metal-contaminated soils. Plant Soil 232:207–214CrossRefGoogle Scholar
  34. Mendez MO, Maier RM (2008) Phytostabilization of mine tailings in arid and semiarid environments-an emerging remediation technology. Environ Health Perspect 116:278–283CrossRefGoogle Scholar
  35. Patel MJ, Patel JN, Subramanian RB (2005) Effect of cadmium on growth and the activity of H2O2 scavenging enzymes in Colocassia esculentum. Plant Soil 273:183–188CrossRefGoogle Scholar
  36. Prasad MNV, Freitas H (2006) Metal tolerant plants: biodiversity prospecting for phytoremediation technology. In: Prasad MNV, Sajwan KS, Naidu R (eds) Trace elements in the environment: biogeochemistry, biotechnology, and bioremediation. Taylor & Francis, Boca Raton, pp 483–506Google Scholar
  37. Pratas J, Favas PJC, D’Souza R, Varun M, Paul MS (2013) Phytoremedial assessment of flora tolerant to heavy metals in the contaminated soils of an abandoned Pb mine in central Portugal. Chemosphere 90:2216–2225CrossRefGoogle Scholar
  38. Pratas J, Favas PJC, D’Souza R, Varun M, Paul MS (2014) Heavy metals in soil and spontaneous flora in a lead mine area, Barbadalhos mine, central Portugal. Comunicações Geológicas 101(Especial II):1047–1050Google Scholar
  39. Saraswat S, Rai JPN (2009) Phytoextraction potential of six plant species grown in multimetal contaminated soil. Chem Ecol 25(1):1–11CrossRefGoogle Scholar
  40. Seregin TV, Ivanov VB (2001) Physiological aspects of toxin action of cadmium and lead on high plants. Plant Physiol 48:606–630Google Scholar
  41. Sharma P, Shanker R (2005) Lead toxicity in plant. Braz J Plant Physiol 17:33–52CrossRefGoogle Scholar
  42. Subin MP, Francis S (2013) Phytotoxic effects of cadmium on seed germination, early seedling growth and antioxidant enzyme activities in Cucurbita maxima Duchesne. Int Res J Biol Sci 2(9):40–47Google Scholar
  43. United States Environmental Protection Agency (USEPA) (2000). Introduction to phytoremediation. EPA 600/R-99/107, U.S. Environmental Protection Agency, Office of Research and Development, Cincinnati, OH, USAGoogle Scholar
  44. Varun M, D’Souza R, Pratas J, Paul MS (2012) Metal contamination of soils and plants associated with the glass industry in North Central India: prospects of phytoremediation. Environ Sci Pollut Res 19(1):269–281CrossRefGoogle Scholar
  45. Varun M, D’Souza R, Favas PJC, Pratas J, Paul MS (2015a) Utilization and supplementation of phytoextraction potential of some terrestrial plants in metal contaminated soils. In: Ansari AA, Gill SS, Gill R, Lanza GR, Newman L (eds.), Phytoremediation: management of environmental contaminants, vol I. Springer International Publishing, New York, pp. 177–200, ISBN 978-3-319-10394-5CrossRefGoogle Scholar
  46. Varun M, Jaggi D, D’Souza R, Paul M, Kumar B (2015b) Abutilon indicum L.: a prospective weed for phytoremediation. Environ Monit Assess 187(8):527. doi: 10.1007/s10661-015-4748-3 CrossRefGoogle Scholar
  47. Yang X, Feng Y, Zhenli H, Stoffella PJ (2005) Molecular mechanisms of heavy metal hyperaccumulation and phytoremediation. J Trace Elem Med Biol 18(4):339–353CrossRefGoogle Scholar
  48. Yourtchi MS, Bayat HR (2013) Effect of cadmium toxicity on growth, cadmium accumulation and macronutrient content of durum wheat (Dena CV). Int Res J Biol Sci 2(9):40–47Google Scholar
  49. Zhuang P, Yang QW, Wang HB, Shu WS (2007) Phytoextraction of heavy metals by eight plant species in the field. Water Air Soil Pollut 184:235–242CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Department of BotanySt. John’s CollegeAgraIndia
  2. 2.Environmental Biology Unit, Department of Plant BiologyUniversity of IlorinIlorinNigeria
  3. 3.School of Life Sciences and the EnvironmentUniversity of Trás-os-Montes e Alto DouroVila RealPortugal
  4. 4.Faculty of Sciences and Technology, MARE – Marine and Environmental Sciences CentreUniversity of CoimbraCoimbraPortugal

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