Evaluation of the Health Status of the Silverside (Odontesthes bonariensis) at a RAMSAR Site in South America

  • M. L. Ballesteros
  • A. C. Hued
  • M. Gonzalez
  • K. S. B. Miglioranza
  • M. A. BistoniEmail author


The objective of this work was to evaluate the health status of an economic and ecologically important fish species from Mar Chiquita Lake, a RAMSAR site located in Cordoba, Argentina, relative to the levels of selected persistent organic pollutants (POPs) in lake water and fish tissues. Odontesthes bonariensis was used as a model species, and its health was estimated by means of histological indices in gills and liver. Sampling was performed according to rainy and dry seasons (i.e. dry, rainy and post-rainy). Gill and liver histopathology were evaluated by semi-quantitative indices and morphometric analysis. Although epithelial lifting in gills and lipid degeneration in liver were frequently registered, they are considered as reversible if environmental conditions improve. During rainy and post-rainy seasons fish presented significantly higher scores of liver and total indices. These higher index scores were correlated with increased levels of POPs in gill and liver tissue. Therefore, preventive measures are needed to mitigate the entry of these compounds into the lake.


Odontesthes bonariensis Histological indices Persistent Organic Pollutants Health status 



This work was supported by grants from the ANCyPT-FONCyT, SECyT-UNC, and National Research Council (CONICET-PIP). The authors thank to Dra. Ma. Angelina Roggio, Dra. Noelia F. Guyón, Dr. Ricardo Torres and Ing. Fernardo Monarde for field and laboratory assistance.


  1. Agency for Toxic Substances and Disease Registry (ATSDR) (2000) Toxicological profile for polychlorinated biphenyls (PCBs). Atlanta, GA.
  2. Ballesteros ML, Bianchi GE, Carranza M, Bistoni MA (2007) Endosulfan acute toxicity and histomorphological alterations in Jenynsia multidentata (Anablepidae, Cyprinodontiformes). J Environ Sci Health B 42:351–357CrossRefGoogle Scholar
  3. Ballesteros ML, Miglioranza KSB, González M et al (2014) Multimatrix measurement of persistent organic pollutants in Mar Chiquita, a continental saline shallow lake. Sci Total Environ 490:73–80CrossRefGoogle Scholar
  4. Bernet D, Schmidt H, Meier W et al. (1999) Histopathology in fish: proposal for a p.rotocol to assess aquatic pollution. J Fish Dis 22:25–34CrossRefGoogle Scholar
  5. Bonansea RI, Amé MV, Wunderlin DA (2013) Determination of priority pesticides in water samples combining SPE and SPME coupled to GC-MS. A case study: Suquía River basin (Argentina). Chemosphere 90:1860–1869CrossRefGoogle Scholar
  6. Boon JP, Lewis WE, Tjoen-A-Choy MR et al (2002) Levels of polybrominated diphenyl ether (PBDE) flame retardants in animals representing different trophic levels of the North Sea food web. Environ Sci Technol 36:4025–4032CrossRefGoogle Scholar
  7. Bucher EH, Etchegoin M (2006) El pejerrey como recurso. In: Bucher EH (ed) Bañados del río Dulce y Laguna Mar Chiquita (Córdoba, Argentina), Primea. Academia Nacional de Ciencias (Córdoba, Argentina), Córdoba, pp 201–217Google Scholar
  8. Carriquiriborde P, Díaz J, López GC et al (2009) Effects of cypermethrin chronic exposure and water temperature on survival, growth, sex differentiation, and gonadal developmental stages of Odontesthes bonariensis (Teleostei). Chemosphere 76:374–380CrossRefGoogle Scholar
  9. Cazenave J, Wunderlin DA, Bistoni M de los, et al (2005) Uptake, tissue distribution and accumulation of microcystin-RR in Corydoras paleatus, Jenynsia multidentata and Odontesthes bonariensis: A field and laboratory study. Aquat Toxicol 75:178–190CrossRefGoogle Scholar
  10. Costa PM, Diniz MS, Caeiro S et al (2009) Histological biomarkers in liver and gills of juvenile Solea senegalensis exposed to contaminated estuarine sediments: a weighted indices approach. Aquat Toxicol 92:202–212CrossRefGoogle Scholar
  11. Da Cuña RH, Rey Vázquez G, Piol MN et al (2011) Assessment of the acute toxicity of the organochlorine pesticide endosulfan in Cichlasoma dimerus (Teleostei, Perciformes). Ecotoxicol Environ Saf 74:1065–1073CrossRefGoogle Scholar
  12. Di Rienzo JA, Casanofes F, Balzarini MG, et al (2016) InfoStat Software Page, InfoStat, CórdobaGoogle Scholar
  13. Erdogrul Ö, Covaci A, Schepens P (2005) Levels of organochlorine pesticides, polychlorinated biphenyls and polybrominated diphenyl ethers in fish species from Kahramanmaras, Turkey. Environ Int 31:703–711CrossRefGoogle Scholar
  14. Fisk AT, Hobson KA, Norstrom RJ (2001) Influence of chemical and biological factors on trophic transfer of persistent organic pollutants in the Northwater Polynya marine food web. Environ Sci Technol 35:732–738CrossRefGoogle Scholar
  15. González M, Miglioranza KSB, Shimabukuro VM et al (2012) Surface and groundwater pollution by organochlorine compounds in a typical soybean system from the south Pampa, Argentina. Environ. Earth Sci 65:481–491CrossRefGoogle Scholar
  16. Hued AC, Oberhofer S, Bistoni MA (2012) Exposure to a commercial glyphosate formulation (Roundup) alters normal gill and liver histology and affects male sexual activity of Jenynsia multidentata (Anablepidae, Cyprinodontiformes). Arch Environ Contam Toxicol 62:107–117CrossRefGoogle Scholar
  17. Linde-Arias AR, Inácio AF, Novo LA et al (2008) Multibiomarker approach in fish to assess the impact of pollution in a large Brazilian river, Paraiba do Sul. Environ Pollut 156:974–979CrossRefGoogle Scholar
  18. Maggioni T, Hued AC, Monferrán MV et al (2012) Bioindicators and biomarkers of environmental pollution in the middle-lower basin of the Suquía River (Córdoba, Argentina). Arch Environ Contam Toxicol 63:337–353CrossRefGoogle Scholar
  19. Mallat J (1985) Fish gill structural changes induced by toxicants and other irritants: a statistical review. Can J Fish Aquat Sci 42:630–648CrossRefGoogle Scholar
  20. Mancini M, Nicola I, Salinas V, Bucco C (2009) Biology of silverside Odontesthes bonariensis (Pisces, Atherinopsidae) in the shallow lake “Los Charos” (Córdoba, Argentina). Rev Peru Biol 15:65–71Google Scholar
  21. Mitsch WJ, Gosselink JG (2000) Wetlands, Third edn. John Wiley & Sons, Inc, New YorkGoogle Scholar
  22. Monferrán MV, Galanti LN, Bonansea RI et al (2011) Integrated survey of water pollution in the Suquía River basin (Córdoba, Argentina). J Environ Monit 13:398–409CrossRefGoogle Scholar
  23. Nero V, Farwell A, Lee LEJ et al (2006) The effects of salinity on naphthenic acid toxicity to yellow perch: gill and liver histopathology. Ecotoxicol Environ Saf 65:252–264CrossRefGoogle Scholar
  24. Pesce SF, Cazenave J, Monferrán MV et al (2008) Integrated survey on toxic effects of lindane on neotropical fish: Corydoras paleatus and Jenynsia multidentata. Environ Pollut 156:775–783CrossRefGoogle Scholar
  25. Rasband WS (2011) ImageJ. v.1.46r.
  26. Rautenberg GE, Amé M V., Monferrán M V., et al (2014) A multi-level approach using Gambusia affinis as a bioindicator of environmental pollution in the middle-lower basin of Suquía River. Ecol Indic 48:706–720CrossRefGoogle Scholar
  27. Ringuelet RA., Arámburu RH., Alonso de Arámburu A (1967) Los peces argentinos de agua dulce. Com. Inv. Cient. Prov. Bs. As., La Plata, Buenos AiresGoogle Scholar
  28. Sagretti L, Bistoni MA (2001) Alimentacion de Odontesthes bonariensis (Cuvier y Valenciennes 1835) (Atheriniformes, Atherinidae) en la laguna Salada de Mar Chiquita (Cordoba, Argentina). Gayana 65:6–11Google Scholar
  29. Silva Barni MF, Ondarza PM, Gonzalez M et al (2016) Persistent organic pollutants (POPs) in fish with different feeding habits inhabiting a shallow lake ecosystem. Sci Total Environ 550:900–909CrossRefGoogle Scholar
  30. Stentiford GD, Longshaw M, Lyons BP et al (2003) Histopathological biomarkers in estuarine fish species for the assessment of biological effects of contaminants. Mar Environ Res 55:137–159CrossRefGoogle Scholar
  31. Subsecretaría de Recursos Hídricos de la Nación (2005) Niveles guía nacionales de calidad de agua ambiente correspondientes a LindanoGoogle Scholar
  32. Troncoso I, Cazenave J, Bacchetta C, Bistoni MA de los (2012) Histopahological changes in the gills and liver of Prochilodus lineatus from the Salado River basin (Santa Fe, Argentina). Fish Physiol Biochem 38:693–702CrossRefGoogle Scholar
  33. Van der Oost R, Beyer J, Vermeulen NPE (2003) Fish bioaccumulation and biomarkers in environmental risk assessment: A review. Environ Toxicol Pharmacol 13:57–149CrossRefGoogle Scholar
  34. Wood CM (2001) Toxic responses of the gill. In: Schlenk D, Benson WH (eds) Target organ toxicity in marine and freshwater teleosts. Taylor and Francis, USA, pp 1–67Google Scholar
  35. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) (2005) Resolución D No 1047: Marco Etico para las Investigaciones BiomédicasGoogle Scholar
  36. UNEP. Stockolm Convention (2005) Eliminando los COP del mundo: guia del convenio de Estocolmo sobre contaminantes orgánicos persistentesGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • M. L. Ballesteros
    • 1
  • A. C. Hued
    • 1
  • M. Gonzalez
    • 2
  • K. S. B. Miglioranza
    • 2
  • M. A. Bistoni
    • 1
    Email author
  1. 1.Instituto de Diversidad y Ecología Animal (CONICET-UNC) and Facultad de Ciencias Exactas Físicas y NaturalesUniversidad Nacional de CórdobaCórdoba,Argentina
  2. 2.Laboratorio de Ecotoxicología y Contaminación Ambiental Funes 3350Instituto de Investigaciones Marinas y Costeras (IIMyC), UNMdP, CONICETMar del PlataArgentina

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