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Effects of Microcystis on Hypothalamic-Pituitary-Gonadal-Liver Axis in Nile Tilapia (Oreochromis niloticus)

  • Jiazhang Chen
  • Shunlong Meng
  • Hai XuEmail author
  • Zhen Zhang
  • Xiangyang Wu
Article

Abstract

In the present study, Nile tilapia (Oreochromis niloticus) were used to assess the endocrine disruption potential of Microcytis aeruginosa. Male Nile tilapia were exposed to lyophilized M. aeruginosa or purified microcystin-LR (8.3 μg/L) for 28 days. The levels of serum hormones (17β-estradiol and testosterone) and transcripts of selected genes in the hypothalamus-pituitary-gonadal-liver axis were analyzed. The results showed that serum hormones were significantly up-regulated, and transcripts of 13 genes (GHRH, PACAP, GH, GHR1, GHR2, IGF1, IGF2, CYP19a, CYP19b, 3β-HSD1, 20β-HSD, 17β-HSD1 and 17β-HSD8) were significantly altered after Microcytis exposure. These results indicate that fish reproduction can be altered in a Microcystis bloom-contaminated aquatic environment.

Keywords

Microcystis MC-LR Brain Hypothalamus-pituitary-gonadal-liver axis Nile tilapia 

Notes

Acknowledgements

This work was supported financially by the National Major Project on Quality & Safety Risk Assessment for Agricultural Products (Grants GJFP2014009), Natural Science Foundation of Jiangsu Province of China (Grants BK20130488).

Supplementary material

128_2017_2051_MOESM1_ESM.docx (18 kb)
Supplementary material 1 (DOCX 18 KB)

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Freshwater Fisheries Research CenterChinese Academy of Fishery SciencesWuxiChina
  2. 2.Key Laboratory of Fishery Eco-environment Assessment and Resource Conservation in Middle and Lower Reaches of the Yangtze RiverCAFSWuxiChina
  3. 3.School of Environment and Safety EngineeringJiangsu UniversityZhenjiangChina

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