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Single Low-Dose Ionizing Radiation Induces Genotoxicity in Adult Zebrafish and its Non-Irradiated Progeny

  • J. Lemos
  • T. Neuparth
  • M. Trigo
  • P. Costa
  • D. Vieira
  • L. Cunha
  • F. Ponte
  • P. S. Costa
  • L. F. Metello
  • A. P. Carvalho
Article

Abstract

This study investigated to what extent a single exposure to low doses of ionizing radiation can induce genotoxic damage in irradiated adult zebrafish (Danio rerio) and its non-irradiated F1 progeny. Four groups of adult zebrafish were irradiated with a single dose of X-rays at 0 (control), 100, 500 and 1000 mGy, respectively, and couples of each group were allowed to reproduce following irradiation. Blood of parental fish and whole-body offspring were analysed by the comet assay for detection of DNA damage. The level of DNA damage in irradiated parental fish increased in a radiation dose-dependent manner at day 1 post-irradiation, but returned to the control level thereafter. The level of DNA damage in the progeny was directly correlated with the parental irradiation dose. Results highlight the genotoxic risk of a single exposure to low-dose ionizing radiation in irradiated individuals and also in its non-irradiated progeny.

Keywords

Zebrafish Ionizing radiation Genotoxicity Comet assay 

Notes

Acknowledgements

We are grateful to the staff of the bioterium of aquatic organisms (BOGA) from CIIMAR and to Júlio Teixeira SA-Radiotherapy Department for their help in zebrafish maintenance and zebrafish irradiation, respectively.

Compliance with Ethical Standards

Ethical Approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • J. Lemos
    • 1
    • 2
  • T. Neuparth
    • 3
  • M. Trigo
    • 4
  • P. Costa
    • 2
  • D. Vieira
    • 2
  • L. Cunha
    • 5
  • F. Ponte
    • 6
  • P. S. Costa
    • 6
  • L. F. Metello
    • 2
    • 5
  • A. P. Carvalho
    • 3
    • 4
  1. 1.ICBAS - Institute of Biomedical SciencesUniversity of PortoPortoPortugal
  2. 2.Nuclear Medicine DepartmentHigh Institute for Allied Health Technologies of Porto - Polytechnic Institute of Porto (ESTSP.IPP)Vila Nova de GaiaPortugal
  3. 3.CIIMAR/CIMAR - Interdisciplinary Centre of Marine and Environmental ResearchUniversity of PortoPortoPortugal
  4. 4.Department of Biology, Faculty of SciencesUniversity of PortoPortoPortugal
  5. 5.IsoPor SAErmesindePortugal
  6. 6.Radiotherapy DeptartmentJúlio Teixeira SAPortoPortugal

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