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Identification of Chromium Resistant Bacteria from Dry Fly Ash Sample of Mejia MTPS Thermal Power Plant, West Bengal, India

Abstract

Eight chromium resistant bacteria were isolated from a dry fly ash sample of DVC-MTPS thermal power plant located in Bankura, West Bengal, India. These isolates displayed different degrees of chromate reduction under aerobic conditions. According to 16S rDNA gene analysis, five of them were Staphylococcus, two were Bacillus and one was Micrococcus. The minimum inhibitory concentration towards chromium and the ability to reduce hexavalent chromium to trivalent chromium was highest in Staphylococcus haemolyticus strain HMR17. All the strains were resistant to multiple heavy metals (As, Cu, Cd, Co, Zn, Mn, Pb and Fe) and reduced toxic hexavalent chromium to relatively non toxic trivalent chromium even in the presence of these multiple heavy metals. All of them showed resistance to different antibiotics. In a soil microcosm study, S. haemolyticus strain HMR17 completely reduced 4 mM hexavalent chromium within 7 days of incubation.

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Acknowledgments

Laboratory facilities were provided by Techno India University. We are grateful to Mrs. Kalyani Pyne, Ex. Engineer (Elec.), EMPC division of DVC MTPS for providing us the fly ash samples.

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Correspondence to Madhumita Roy.

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Roychowdhury, R., Mukherjee, P. & Roy, M. Identification of Chromium Resistant Bacteria from Dry Fly Ash Sample of Mejia MTPS Thermal Power Plant, West Bengal, India. Bull Environ Contam Toxicol 96, 210–216 (2016). https://doi.org/10.1007/s00128-015-1692-4

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Keywords

  • Dry fly ash (DFA)
  • Chromate reducing bacteria
  • Minimal inhibitory concentration (MIC)
  • Multiple heavy metals
  • Antibiotic resistance
  • Soil microcosm