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Physiological Responses in Chinese Rare Minnow Larvae Following Exposure to Low-Dose Tributyltin

Abstract

In the present study, the antioxidant response and acetylcholinesterase (AChE) activity were measured in Chinese rare minnow larvae (Gobiocypris rarus) after exposure to tributyltin (TBT) (0, 100, 400 and 800 ngL−1) for 7 days, as well as the expression of a series of genes, including cr, aptase and prl genes involved in the ion-regulatory process and igfbp3 and gh related to growth rate. Results shows that oxidative stress was generated in fish exposed to TBT, as evidenced by elevated malondialdehyde levels and the inhibition of antioxidant parameters. The activity of acetylcholinesterase (AChE) was also inhibited in fish under higher TBT stress. Moreover, genes involved in ion regulation and growth were affected, based on the regulated transcription of the cr, atpase, gh, prl and igfbp3 genes in the treated groups. The observed effects of TBT upon antioxidant responses and altered expression of genes provides insight into the use of these molecular biomarkers in evaluating mechanisms of TBT toxicity in fish.

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Acknowledgments

This study was supported by Special Scientific Research Funds for Central Non-profit Institutes, Chinese Academy of Fishery Sciences (2014A02YQ01), Technology Foundation for Selected Overseas Chinese Scholar of MOHRSS, and the Ministry of Education, Youth and Sports of the Czech Republic – projects “CENAKVA” (No. CZ.1.05/2.1.00/01.0024) and “CENAKVA II” (No. LO1205 under the NPU I program).

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Correspondence to Zhi-Hua Li.

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Li, P., Li, Z. Physiological Responses in Chinese Rare Minnow Larvae Following Exposure to Low-Dose Tributyltin. Bull Environ Contam Toxicol 95, 588–592 (2015). https://doi.org/10.1007/s00128-015-1655-9

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Keywords

  • Tributyltin
  • Chinese rare minnow
  • Physiological responses
  • Gene expression