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Expression Profile of Antioxidant Enzymes in Hemocytes from Freshwater Prawn Macrobrachium rosenbergii Exposed to an Elevated Level of Copper

  • Hui Guo
  • Yu-Tao Miao
  • Jian-An Xian
  • Kun Qian
  • An-Li WangEmail author
Article

Abstract

This study evaluated the expression level of antioxidant enzymes Cu, Zn-superoxide dismutase (Cu, Zn-SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione S-transferase (GST) in hemocytes of Macrobrachium rosenbergii exposed to copper by real-time PCR (qRT-PCR). Results showed that the mRNA expression of Cu, Zn-SOD increased to reach a peak at 6 h, then recovered to its normal level at 48 h. CAT expression level was significantly increased at 12 h and reached a peak at 24 h, but recovered to its normal level later. GPx expression level was significantly increased at 6 h and reached the peak at 12 h. GST expression level was significantly induced from 12 to 24 h and then dropped to its normal level at 48 h. These results indicated that antioxidant enzymes were inducible, possibly for removing excessive reactive oxygen species to protect prawn from oxidative stress.

Keywords

Macrobrachium rosenbergii Copper Antioxidant enzyme Gene expression Hemocyte 

Notes

Acknowledgments

This research was supported by the Scientific Research Foundation of Graduate School of South China Normal University (2013kyjj004), the National Natural Science Foundation of China (31302164), Guangdong Provincial Natural Science Foundation (S2012040008093).

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Hui Guo
    • 1
    • 2
  • Yu-Tao Miao
    • 1
  • Jian-An Xian
    • 3
  • Kun Qian
    • 1
  • An-Li Wang
    • 1
    Email author
  1. 1.Key Laboratory of Ecology and Environmental Science of Guangdong Higher Education Institutes, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, School of Life ScienceSouth China Normal UniversityGuangzhouPeople’s Republic of China
  2. 2.Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guang-dong Higher Education Institutes, College of FisheriesGuangdong Ocean UniversityZhanjiangPeople’s Republic of China
  3. 3.Institute of Tropical Bioscience and BiotechnologyChinese Academy of Tropical Agricultural SciencesHaikouPeople’s Republic of China

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