Expression Profile of Antioxidant Enzymes in Hemocytes from Freshwater Prawn Macrobrachium rosenbergii Exposed to an Elevated Level of Copper
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This study evaluated the expression level of antioxidant enzymes Cu, Zn-superoxide dismutase (Cu, Zn-SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione S-transferase (GST) in hemocytes of Macrobrachium rosenbergii exposed to copper by real-time PCR (qRT-PCR). Results showed that the mRNA expression of Cu, Zn-SOD increased to reach a peak at 6 h, then recovered to its normal level at 48 h. CAT expression level was significantly increased at 12 h and reached a peak at 24 h, but recovered to its normal level later. GPx expression level was significantly increased at 6 h and reached the peak at 12 h. GST expression level was significantly induced from 12 to 24 h and then dropped to its normal level at 48 h. These results indicated that antioxidant enzymes were inducible, possibly for removing excessive reactive oxygen species to protect prawn from oxidative stress.
KeywordsMacrobrachium rosenbergii Copper Antioxidant enzyme Gene expression Hemocyte
This research was supported by the Scientific Research Foundation of Graduate School of South China Normal University (2013kyjj004), the National Natural Science Foundation of China (31302164), Guangdong Provincial Natural Science Foundation (S2012040008093).
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