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Determination and Method Validation of the New Sulfone Fungicide 2-(4-Fluorophenyl)-5-Methylsulfonyl-1,3,4-Oxadiazole in Tomato and Soil by UPLC in Field Trial Samples from Guizhou Province, China


A new method was developed and validated for the determination of 2-(4-fluorophenyl)-5-methylsulfonyl-1,3,4-oxadiazole (jiahuangxianjunzuo, JHXJZ) by ultra-performance liquid chromatography equipped with photo-diode array detector. JHXJZ from tomato and soil was extracted with ethyl acetate without further cleanup. The limits of detection and quantification of JHXJZ were 0.0083 and 0.025 mg kg−1 in tomato, 0.0017 and 0.005 mg kg−1 in soil, respectively. The average recoveries of tomato and soil were studied at three spiked levels and ranged from 84.51 % to 101.30 % and 85.30 % to 101.53 %, respectively, with relative standard deviations of 2.61 %–4.13 % and 1.21 %–4.80 %, respectively. The results indicated that the reported method could meet the requirement for the analysis of JHXJZ in trace amount in tomato and soil.

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  1. Almeida C, Serôdio P, Florêncio MH, Nogueira JMF (2007) New strategies to screen for endocrine-disrupting chemicals in the Portuguese marine environment utilizing large volume injection–capillary gas chromatography–mass spectrometry combined with retention time locking libraries (LVI–GC–MS–RTL). Anal Bioanal Chem 387:2569–2583

  2. Al-Mutlaq KF (2006) Characteristics and alteration of pesticide residues in surface soils of agricultural fields and public parks. Environ Geol 51:493–497

  3. Andreu V, Picó Y (2012) Determination of currently used pesticides in biota. Anal Bioanal Chem 404:2659–2681

  4. Chen ZL, Zhang H, Liu B, Yang GS, Aboul-Enein HY, Wang WB, Ding RY, Du HX, Li HD (2007) Determination of herbicide residues in garlic by GC–MS. Chromatographia 66:887–891

  5. Crane DB, Younghans-Haug C (1992) Oxadiazon residue concentrations in sediment, fish, and shellfish from a combined residential/agricultural area in Southern California. Bull Environ Contam Toxicol 48:608–615

  6. Dong J, Pan YX, Lv JX, Sun J, Gong XM, Li K (2011) Multiresidue method for the determination of pesticides in fruits and vegetables using gas chromatography–negative chemical ionization-triple quadrupole tandem mass spectrometry. Chromatographia 74:109–119

  7. Du HX, Fang LP, Chen ZL, Li HD, Wang FE, Dong Z (2014) Study on residue and degradation of oxadiazon in cotton and soil. Shandong Agric Sci 46(6):108–110, 115

  8. Guardigli A, Lefar MS, Gallo MA, Laurent M, Buys M (1976) Residue uptake and depletion measurements of dietary oxadiazon in mammalian and avian species. Arch Environ Contam Toxicol 4:145–154

  9. Kamal A, Shaik AB, Reddy GN, Kumar CG, Joseph J, Kumar GB, Purushotham U, Sastry GN (2014) Synthesis, biological evaluation, and molecular modeling of (E)-2-aryl-5-styryl-1,3,4-oxadiazole derivatives as acetylcholine esterase inhibitors. Med Chem Res 23:2080–2092

  10. Navalón A, Prieto A, Araujo L, Vílchez JL (2001) Determination of tebufenpyrad and oxadiazon by solid-phase microextraction and gas chromatography–mass spectrometry. Chromatographia 54:377–382

  11. Odanaka Y, Matano O, Goto S (1991) The use of solid bonded-phase extraction as alternative to liquid–liquid partitioning for pesticide residue analysis of crops. Fresenius J Anal Chem 339:368–373

  12. Tsuda T, Kojima M, Harada H, Nakajima A, Aoki S (1998) Pesticides and their oxidation products in water and fish from rivers flowing into Lake Biwa. Bull Environ Contam Toxicol 60:151–158

  13. Xie YF, Yu JL, Song GC, Li RJ, Wang JX, Bi YL (2012) Determination of oxadiargyl residues in potato and soil by gas chromatography. Pestic Sci Adm 33(3):28–33

  14. Xu XL, Li L, Zhong WK, He YJ (2009) Multi-residue analysis of 205 crop pesticides using mini-solid phase extraction–large volume injection–GC–MS. Chromatographia 70:173–183

  15. Xu WM, Han FF, He M, Hu DY, He J, Yang S, Song BA (2012) Inhibition of tobacco bacterial wilt with sulfone derivatives containing an 1,3,4-oxadiazole moiety. J Agric Food Chem 60:1036–1041

  16. Yan H, Shao MY, Liang ZF, Zhang HQ, Yu AM (2013) Determination of four pesticides in soil by homogeneous ionic liquid-based microextraction coupled with high-performance liquid chromatography. Chem Res Chin Univ 29(2):218–222

  17. Zou R, Yang RB, Fu Q, Jiang B, Peng S (2013) Residue analysis of oxadiazon in rice field. Agrochemicals 52(5):363–365

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The authors thank the Special Fund for Agro-scientific Research in the Public Interest (No. 201203022), the Program of Science Foundation of Guizhou Province (No. 20132106), the National Natural Science Foundation of China (No. 21402034), the Program of Talents Science of Guizhou University (No. 201330), the Innovation Foundation of Guizhou University (No. 2014075) and the Science and Technology Programs of Guizhou Province (No. 20147664).

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Correspondence to Kankan Zhang or Deyu Hu.

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The authors declare that the experiments comply with the current laws of China in which they were performed.

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Linghu, R., Jin, M., Pan, S. et al. Determination and Method Validation of the New Sulfone Fungicide 2-(4-Fluorophenyl)-5-Methylsulfonyl-1,3,4-Oxadiazole in Tomato and Soil by UPLC in Field Trial Samples from Guizhou Province, China. Bull Environ Contam Toxicol 95, 373–378 (2015). https://doi.org/10.1007/s00128-015-1597-2

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  • New sulfone fungicide
  • Jiahuangxianjunzuo
  • Recovery
  • Tomato
  • Soil
  • Validation
  • UPLC