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Herbicide Clomazone Effects on δ-Aminolevulinic Acid Activity and Metabolic Parameters in Cyprinus carpio

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The objective of this study was to investigate δ-aminolevulinic acid (δ-ALA-D) activity and metabolic parameters of Cyprinus carpio exposed to clomazone herbicide. Fish were exposed 2.5, 5, 10 and 20 mg L−1 of clomazone for 192 h. Results indicated that δ-ALA-D activity was decreased in the gills at concentrations of 5 and 10 mg L−1. Liver glycogen increased, while muscle and gill glycogen levels decreased at 5, 10 and 20 mg L−1. Glucose was increased in the gills and plasma. Lactate decreased in the gills and liver and increased in the muscle. Protein and amino acids levels increased in the liver and gills and decreased in the muscle. At a clomazone concentration of 20 mg L−1, ammonia increased in the gills and muscle and decreased in the liver. The results indicated that the metabolic parameters of glycogen, lactate, protein and amino acids in liver, muscle and gills, blood glucose levels, and the enzyme δ-ALA-D in gills may be useful indicators of clomazone toxicity in carp.

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  1. Barbosa NBV, Rocha JBT, Zeni G, Emanuelli T, Beque MC, Braga AL (1998) Effect of organic forms of selenium on δ-aminolevulinate dehydratase from liver, kidney, and brain of adult rats. Toxicol Appl Pharmacol 149:243–253

  2. Begum G (2004) Carbofuran insecticide induced biochemical alterations in liver and muscle tissues of the fish Clarias batrachus (linn) and recovery response. Aquat Toxicol 66:83–92

  3. Boyd CE, Tucker CS (1992) Water quality and pond soil analyses for aquaculture. Auburn University, Auburn, p 183

  4. Campana O, Sarasquete C, Blasco J (2003) Effect of on ALA-D activity, metallothionein levels, and lipid peroxidation in blood, kidney, and liver of the toadfish Halobatrachus didactylus. Ecotoxicol Environ Saf 55:116–125

  5. Cattaneo R, Loro VL, Spanevello R, Silveira FA, Luz L, Miron DS, Fonseca MB, Moraes BS, Clasen B (2008) Metabolic and histological parameters of silver catfish (Rhamdia quelen) exposed to commercial formulation of 2,4-dichlorophenoxiacetic acid (2,4-D) herbicide. Pestic Biochem Physiol 92:133–137

  6. Cattaneo R, Moraes BS, Loro VL, Pretto A, Menezes C, Sartori GMS, Clasen B, Avila LA, Marchesan E, Zanella R (2012) Tissue biochemical alterations of Cyprinus carpio exposed to commercial herbicide containing clomazone under rice-field conditions. Arch Environ Contam Toxicol 62:97–106

  7. Crestani M, Menezes C, Glusczak L, Miron DS, Lazzari R, Duarte MF, Morsch VM, Pippi AL, Vieira VP (2006) Effects of clomazone herbicide on hematological and some parameters of protein and carbohydrate metabolism of silver catfish Rhamdia quelen. Ecotoxicol Environ Saf 65:48–55

  8. Glusczak L, Miron DS, Moraes BS, Simões RR, Schetinger MRC, Morsch VM, Loro VL (2007) Acute effects of glyphosate herbicide on metabolic and enzymatic parameters of silver catfish (Rhamdia quelen). Comp Biochem Physiol C 146:519–524

  9. Jaffe EK (2000) The porphobilinogen synthase family of metalloenzymes. Acta Crystallogr 56:115–128

  10. Moraes BS, Loro VL, Pretto A, Fonseca MB, Menezes C, Marchezan E, Reimche GB, Avila LA (2009) Toxicological and metabolic parameters of the teleost fish (Leporinus obtusidens) in response to commercial herbicides containing clomazone and propanil. Pestic Biochem Physiol 95:57–62

  11. Pereira L, Fernandes MN, Martinez CBR (2013) Hematological and biochemical alterations in the fish Prochilodus lineatus caused by the herbicide clomazone. Environ Toxicol Pharmacol 36:1–8

  12. Sahib IKA, Sambasiva-Rao KRS, Ramana-Rao KV (1984) Effect of malathion on protein synthetic potentiality of the tissues of the teleost, Tilapia mossambica (Peters), as measured through incorporation of [14C] amino acids. Toxicol Lett 20:63–67

  13. Sassa S, Fujita H, Kappas A (1989) Genetic and chemical influences on heme biosynthesis. In: Kotyk A, Skoda J, Paces V, Kostka V (eds) Highlights of modern biochemistry, vol 1. VSP, Utrecht, pp 329–338

  14. Soares FA, Farina M, Boettcher A, Braga AL, Rocha JBT (2005) Organic and inorganic forms of selenium inhibited differently fish (Rhamdia quelen) and rat (Rattus norvergicus albinus) δ-aminolevulinate dehydratase. Environ Res 98:46–54

  15. Zanella R, Primel EG, Machado SLO, Gonçalves FF, Marchezan E (2002) Monitoring of the herbicide clomazone in environmental water samples by solid-phase extraction and high-performance liquid chromatography with ultraviolet detection. Chromatographia 55:573–577

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C.C.M. received fellowship of CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil), V.L.L. and N.B.V.B. received CNPq (Conselho Nacional de Pesquisa e Desenvolvimento Científico) research fellowship.

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Correspondence to Vania Lucia Loro.

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Menezes, C., Leitemperger, J., Murussi, C. et al. Herbicide Clomazone Effects on δ-Aminolevulinic Acid Activity and Metabolic Parameters in Cyprinus carpio . Bull Environ Contam Toxicol 92, 393–398 (2014).

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  • δ-Aminolevulinic acid
  • Carp
  • Clomazone
  • Metabolic parameters