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Effects of Petroleum Hydrocarbon Contaminated Soil on Germination, Metabolism and Early Growth of Green Gram, Vigna radiata L.

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The objective of the present study was to evaluate effects of petroleum hydrocarbon contaminated soil on the leguminous plant, Vigna radiata L. Seed germination, metabolism and early growth performance of V. radiata L. were studied as parameters by applying a combined approach. The employed combined method which included microcalorimetry and analysis of the root cross section revealed dose dependent effects of petroleum hydrocarbon contaminated soil on V. radiata L. for most parameters. Although significant reductions in measured parameters were observed even at low total petroleum hydrocarbon (TPH) levels such as 1 % and 1.5 %, calculated inhibitions, IC50 values and metabolic heat emission-time curves inferred that substantial negative effects can be expected on V. radiata L. in soils with comparatively high contamination levels, such as 2.5 % TPH and higher.

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  1. Achuba FI (2006) The effect of sublethal concentrations of crude oil on the growth metabolism of cowpea (Vigna unguiculata) seedlings. Environmentalist 26:17–20

  2. Adam G, Ducan HJ (2002) Influence on diesel fuel on seed germination. Environ Pollut 120:363–370

  3. Ahemad M, Khan MS (2012) Alleviation of fungicide-induced phytotoxicity in greengram (Vigna radiata (L.) Wilczek) using fungicide-tolerant and growth promoting Pseudomonas strain. Saudi J Biol Sci 19:451–459

  4. Al-Mutairi N, Bufarsan A, Al-Rukaibi F (2008) Ecorisk evaluation and treatability potential of soils contaminated with petroleum hydrocarbon based fuels. Chemosphere 74:142–148

  5. Andreoni V, Cavalca L, Rao MA, Nocerino G, Bernasconi S, Della Mico E, Colombo M, Gianfreda L (2004) Bacterial communities and enzyme activities of PAHs polluted soil. Chemosphere 57:401–412

  6. Banks MK, Schultz KE (2005) Comparison of plants for germination toxicity tests in petroleum- contaminated soils. Water Air Soil Pollut 167:211–219

  7. Chaudhary SK, Rai UN, Mishra K, Huang HG, Yang XE, Inouhe M, Gupta DK (2011) Growth and metal accumulation potential of Vigna radiata L. grown under fly- ash amendments. Ecol Eng 37:1583–1588

  8. Chupakhina GN, Maslennikov PV (2004) Plant adaptation to oil stress. J Ecol 35:290–295

  9. De Jong E (1980) The effect of a crude oil spill on cereals. Environ Pollut 22:187–196

  10. Dorn PB, Salanitro JP (2000) Temporal ecological assessment of oil contaminated soils before and after bioremediation. Chemosphere 40:419–426

  11. Edelstein M, Bradford KJ, Burger DW (2001) Metabolic heat and CO2 production rates during germination of melon (Cucumis melo L.) seeds measured by microcalorimetry. Seed Sci Res 11:265–272

  12. Gerhardt KE, Hung XD, Glick BR, Greenberg BM (2009) Phytoremediation and rhizoremediation of organic soil contaminants: potential and challenges. Plant Sci 176:20–30

  13. Hodgson E (2004) A text book of modern toxicology, 3rd edn. Wiley, Hoboken, p 221

  14. Inckot RC, Santos GO, Souza LAD, Bona C (2011) Germination and development of Mimosa pilulifera in petroleum-contaminated soil and bioremediated soil. Flora 206:261–266

  15. ISO (1993b) ISO 11269-1. Soil quality: Determination of the pollutants effect on soil flora. Part 1. Method for the measurement of inhibition of root growth. International Organization for Standardization, Geneva

  16. Kaneko S, Inagaki M, Morishita T (2010) A simple method for the determination of nitrate in potassium chloride extracts from forest soils.19th World Congress of Soil Science, Soil Solution for a Changing World, Brisbane, Australia, Published on DVD

  17. Kazi TG, Jamali MK, Arain MB, Afridi HI, Jalbani N, Sarfraz RA, Ansari R (2009) Evaluation of an ultrasonic acid digestion procedure for total heavy metals. J Hazard Mater 161:1391–1398

  18. Kopponen TH, Jaakkola T, Keina MM, Toivola N, Kaipainen S, Tuomainen J, Servomaa K, Martikainen JP (2006) Microbial communities, biomass, and activities in soils as affected by freeze thaw cycles. Soil Biol Biochem 38:1861–1871

  19. Kupidlowska E, Gniazdowska A, Stepien J, Corbineau F, Vinel D, Skoczowski A, Janeczko A, Bogatek R (2006) Impact of sunflower (Helianthus annuus L.) extracts upon reserve mobilization and energy metabolism in germinating mustard (Sinapis alba L.) seeds. J Chem Ecol 32:2569–2583

  20. Leitgib L, Kalman J, Gruize K (2007) Comparison of bioassays by testing whole soil and their water extract from contaminated sites. Chemosphere 66:428–434

  21. Maclachalam S, Zalik S (1963) Plastid structure, chlorophyll concentration and free amino acid composition of a chlorophyll mutant of barley. Can J Bot 41:1053–1062

  22. Mehlich A (1985) Mehlich 3 soil test extractant: a modification of Mehlich 2 extractant. Commun Soil Sci Plant 15:1409–1416

  23. Merkl N, Schultze-Kraft R, Infante C (2004) Phytoremediation in the tropics-the effect of crude oil on the growth on tropical plants. Bioremediat J 8:177–184

  24. Merkl N, Schultze-Kraft R, Infante C (2005) Phytoremediation of petroleum-contaminated soils in the tropics-assessment of tropical grasses and legumes for enhancing oil-degradation. Water Air Soil Pollut 165:195–209

  25. Naidoo G (2010) Responses of the mangroves Avicennia marina and Bruguiera gymnorrhiza to oil contamination. Flora 205:356–361

  26. Nelson DW, Sommers LE (1996) Total carbon, organic carbon, and organic matter. In: Page AL, Methods of soil analysis Part 2, 2nd edn. Ed. Agronomy. 9:961–1010. Am. Soc. of Agron., Inc. Madison

  27. Piechalak A, Tomaszewska B, Baralkiewicz D, Malecka A (2002) Accumulation and detoxification of lead ions in legumes. Phytochemistry 60:153–162

  28. Reynoso-Cuevas L, Gallegos-Martinez ME, Cruz-Sosa F, Gutierrez-Rojas M (2008) In vitro evaluation of germination and growth of five plant species on medium supplemented with hydrocarbons associated with contaminated soil. Bioresour Technol 99:6379–6385

  29. Singh RP, Agrawal M (2010) Effect of different sewage sludge applications on growth and yield of Vigna radiata L. field crop: metal uptake by plant. Ecol Eng 36:969–972

  30. Singh RP, Tripathi RD, Dabas S, Rizvi SMH, Ali MB, Sinha SK, Gupta DK, Mishra S, Rai UN (2003) Effect of lead on growth and nitrate assimilation of Vigna radiata (L.) Wilczek seedlings in a salt affected environment. Chemosphere 52:1245–1250

  31. Smith NB, Criddle RS, Hansen LD (2000) Plant growth, respiration and environmental stress. J Plant Biol 27:89–97

  32. Tang J, Lu X, Sun Q, Zhu W (2012) Ageing of petroleum hydrocarbons in soil under different attenuation conditions. Agric Ecosyst Environ 149:109–117

  33. Wang Y, Oyaizu H (2009) Evaluation of the phytoremediation potential of four plant species for dibenzofuran-contaminated soil. J Hazard Mater 168:760–764

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This work was supported in part by grants from the International Joint Key Project from Chinese Ministry of Science and Technology (2010DFA12780), International Joint Key Project from National Natural Science Foundation of China (40920134003), National Natural Science Foundation of China (41273092) and National Outstanding Youth Research Foundation of China (40925010). KM acknowledges the financial support by the Chinese Government Scholarship through Chinese Scholarship Council.

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Correspondence to Jun Yao.

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Masakorala, K., Yao, J., Chandankere, R. et al. Effects of Petroleum Hydrocarbon Contaminated Soil on Germination, Metabolism and Early Growth of Green Gram, Vigna radiata L.. Bull Environ Contam Toxicol 91, 224–230 (2013).

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  • Petroleum contaminated soil
  • Vigna radiata L.
  • Metabolism
  • Microcalrimetry