Simultaneous Determination of 15 Multiresidue Organophosphorous Pesticides in Camellia Oil by MSPD-GC–MS

  • Yihua Liu
  • Danyu Shen
  • Runhong Mo
  • Donglian Zhong
  • Fubin TangEmail author


A one step method based on matrix solid-phase dispersion for simultaneous determination of 15 organophosphorous pesticide residues in camellia oil was developed. The sample preparation could finish in 5 min without extraction procedure, and then analyzed by gas chromatograph-mass spectrometer. Average recoveries ranged between 73.2 % and 108.6 %, with relative standard deviation values (intra-day and inter-day) lower than 16 % at two concentration levels. The method limit of detection was 5 ng/g, which could meet the regulatory maximum residue limits for the pesticides.


Pesticide residue Camellia oil MSPD GC–MS 



This work was supported by the Applied Research Project in the Public Interest of Zhejiang Province (2012C22090) and Special Fund for Forestry Scientific Research in the Public Interest (201204414, 201304705).


  1. Benincasa C, Perri E, Iannotta N, Scalercio S (2011) LC/ESI-MS/MS method for the identification and quantification of spinosad residues in olive oils. Food Chem 125:1116–1120CrossRefGoogle Scholar
  2. Dong NT, Hee LM, Ho LG (2010) Rapid determination of 95 pesticides in soybean oil using liquid–liquid extraction followed by centrifugation, freezing and dispersive solid phase extraction as cleanup steps and gas chromatography with mass spectrometric detection. Microchem J 95:113–119CrossRefGoogle Scholar
  3. Garcia-Reyes JF, Ferrer C, Gomez-Ramos MJ, Molina-Diaz A, Fernandez-Alba AR (2007) Determination of pesticide residues in olive oil and olives. Trends Anal Chem 26:239–251CrossRefGoogle Scholar
  4. Harley KG, Huen K, Schall RA, Holland NT, Bradman A, Barr DB, Eskenazi B (2011) Association of organophosphate pesticide exposure and paraoxonase with birth outcome in Mexican-American women. Plos One 6(8):e23923CrossRefGoogle Scholar
  5. Hayden KM, Norton MC, Darcey D, Ostbye T, Zandi PP, Breitner JCS, Welsh-Bohmer KA, Investigators CCS (2010) Occupational exposure to pesticides increases the risk of incident AD the cache county study. Neurology 74:1524–1530CrossRefGoogle Scholar
  6. Li H, Zhou GY, Zhang HY, Liu JA (2011) Research progress on the health function of tea oil. J Med Plant Res 5:485–489Google Scholar
  7. Moore PD, Patlolla AK, Tchounwou PB (2011) Cytogenetic evaluation of malathion-induced toxicity in Sprague-Dawley rats. Mutat Res 725:78–82CrossRefGoogle Scholar
  8. Mu GF, Liu HT, Xu LN, Tian LH, Luan F (2012) Matrix solid-phase dispersion extraction and capillary electrophoresis determination of tetracycline residues in milk. Food Anal Meth 5:148–153CrossRefGoogle Scholar
  9. Perez-Parada A, Colazzo M, Besil N, Geis-Asteggiante L, Rey F, Heinzen H (2011) Determination of coumaphos, chlorpyrifos and ethion residues in propolis tinctures by matrix solid-phase dispersion and gas chromatography coupled to flame photometric and mass spectrometric detection. J Chromatogr A 1218:5852–5857CrossRefGoogle Scholar
  10. Rodrigues SA, Caldas SS, Primel EG (2010) A simple; efficient and environmentally friendly method for the extraction of pesticides from onion by matrix solid-phase dispersion with liquid chromatography-tandem mass spectrometric detection. Anal Chim Acta 678:82–89CrossRefGoogle Scholar
  11. Zainudin BH, Abubakar NK, Muhamad H (2009) Determination of cypermethrin in palm oil matrices. Eur J Lipid Sci Technol 111:1014–1019CrossRefGoogle Scholar
  12. Zhang Y, Chen QP, Luo XW, Dai TT, Lu BQ, Shen JF (2011) Mutagenicity and safety evaluation of the water extract of Camellia oleifera Abel. J Food Sci 76:T84–T89CrossRefGoogle Scholar
  13. Zhang YP, Xu X, Qi X, Gao WQ, Sun S, Li XT, Jiang CF, Yu AM, Zhang HQ, Yu Y (2012) Determination of sulfonamides in livers using matrix solid-phase dispersion extraction high-performance liquid chromatography. J Sep Sci 35:45–52CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Yihua Liu
    • 1
  • Danyu Shen
    • 1
  • Runhong Mo
    • 1
  • Donglian Zhong
    • 1
  • Fubin Tang
    • 1
    Email author
  1. 1.Research Institute of Subtropical ForestryChinese Academy of ForestryFuyangPeople’s Republic of China

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