Acute Toxicity of Fipronil to the Stingless Bee Scaptotrigona postica Latreille

  • Cynthia Renata Oliveira Jacob
  • Hellen Maria Soares
  • Stephan Malfitano Carvalho
  • Roberta Cornélio Ferreira Nocelli
  • Osmar Malaspina
Article

Abstract

Fipronil is an insecticide widely used to control a great number of pests, thus the aim of this study was to determine the lethal dose and lethal concentration (LD50 and LC50) of this insecticide to the stingless bees Scaptotrigona postica Latreille, 1807. The LD50 and LC50 values obtained after 24 h of exposition were of 0.54 ng a.i./bee and 0.24 ng a.i./μL diet, respectively. These values were considered highly toxic to stingless bees.

Keywords

Phenylpyrazole Wild bee Lethal dose Lethal concentration 

References

  1. Aliouane Y, Hassani AKE, Gary V, Armengaud C, Lambin M, Gauthier M (2009) Subchonic exposure of honeybees to sublethal doses of pesticides: effects on behavior. Environ Toxicol Chem 28:113–122CrossRefGoogle Scholar
  2. Bernal J, Martin-Hernandez R, Diego JC, Nozal MJ, Gonzalez-Porto AV, Bernal JL, Higes M (2011) An exposure study to assess the potential impact of fipronil in treated sunflower seeds on honey bee colony losses in Spain. Pest Manag Sci 67:1320–1331CrossRefGoogle Scholar
  3. Brittain CA, Vighi M, Bommarco R, Settele J, Potts SG (2010) Impacts of a pesticide on pollinator species richness at different spatial scales. Basic Appl Ecol 11:106–115CrossRefGoogle Scholar
  4. Caboni P, Sammelson RE, Casida JE (2003) Phenylpyrazole insecticide photochemistry, metabolism, and GABAergic action: ethiprole compared with fipronil. J Agric Food Chem 51:7055–7061CrossRefGoogle Scholar
  5. Carvalho SM, Carvalho GA, Carvalho CF, Bueno Filho JSS, Baptista APM (2009) Toxicidade de acaricidas/inseticidas empregados na citricultura para abelha africanizada Apis mellifera L. 1758 (Hymenoptera: Apidae). Arq Inst Biol 76:597–606Google Scholar
  6. Cluzeau S (2002) Risk assessment of plant protection products on honey bees: regulatory aspects. In: Devillers J, Pham-Delègue MH (eds) Honey bees: Estimating the environmental impact of chemicals. Taylor & Francis, London, pp 42–55CrossRefGoogle Scholar
  7. Decourtye A, Devillers J, Genecque E, Le Menach K, Budzinski H, Cluzeau S, Pham-Delègue M (2005) Comparative sublethal toxicity of nine pesticides on olfactory learning performances of the honeybee Apis mellifera. Archiv Environ Contam Toxicol 48:242–250CrossRefGoogle Scholar
  8. Decourtye A, Lefort S, Devillers J, Gauthier M, Aupinel P, Tisseur M (2009) Sublethal effects of fipronil on the ability of honeybees (Apis mellifera L.) to orientate in a complex maze. Julius-Kühn-Archiv 423:75–83Google Scholar
  9. Devillers J (2002) The ecological importance of honey bees and their relevance to ecotoxicology. In: Devillers J, Pham-Delègue MH (eds) Honey Bees Estimating the Environmental Impact of Chemicals. Taylor & Francis, London, pp 1–332CrossRefGoogle Scholar
  10. El Hassani AKE, Dacher M, Gauthier M, Armengaud C (2005) Effects of sublethal doses of fipronil on the behavior of the honeybee (Apis mellifera). Pharmacol Biochem Behav 82:30–39CrossRefGoogle Scholar
  11. Ferreira RAC, Silva-Zacarin EMC, Tomotake MEM, Malaspina O (2012) (No prelo) Cellular responses in the Malpighian tubules of Scaptotrigona postica (Latreille, 1807) exposed to low doses of fipronil and boric acid. Micron, in pressGoogle Scholar
  12. Hainzl D, Casida JE (1996) Fipronil insecticide: novel photochemical desulfinylation with retention of neurotoxicity. PNAS 93:12764–12767CrossRefGoogle Scholar
  13. Imperatriz-Fonseca VL (2010) Polinização: Os desafios de um Brasil biodiverso para o uso dos serviços ambientais prestados pelas abelhas. In: Ribeiro MF (ed), Segunda Semana dos polinizadores. (pp. 49–59). Petrolina: Embrapa Semiárido-Documentos, p 229.Google Scholar
  14. Ishaaya I (2001) Biochemical processes related to insecticide action: an overview. In: Ishaaya I (ed) Biochemical sites of insecticide action and resistance. Springer, Berlin, pp 1–10CrossRefGoogle Scholar
  15. Lourenço CT, Carvalho SM, Malaspina O, Nocelli RCF (2012) Oral toxicity of fipronil insecticide against the stingless bee Melipona scutelaris (Latreilli, 1811). Bull Environ Contam Toxicol 89:921–924CrossRefGoogle Scholar
  16. Macieira OJD, Proni EA (2004) Capacidade de resistência a altas e baixas temperaturas em operárias de Scaptotrigona postica (Latreille) (Hymenoptera, Apidae) durante os períodos de verão e inverno. Rev Bras Zool 21:893–896CrossRefGoogle Scholar
  17. Mayer DF, Lunden JD (1999) Field and laboratory tests of the effects of fipronil on adult female bees of Apis mellifera, Megachile rotundata and Nomia melanderi. J Apic Res 38:191–197Google Scholar
  18. Moraes SS, Bautista ARL, Viana BF (2000) Avaliação da toxicidade aguda (DL50 e CL50) de inseticidas para Scaptotrigona tubiba (Smith) (Hymenoptera: Apidae): via de contato. An Soc Entomol Brasil 29:32–37CrossRefGoogle Scholar
  19. Organization for Economic Cooperation and Development (OECD) (1998a) Honeybees, acute contact toxicity test. OECD guidelines for the testing of chemicals, p 214Google Scholar
  20. Organization for Economic Cooperation and Development (OECD) (1998b) Honeybees, acute oral toxicity test. OECD guidelines for the testing of chemicals, p 213Google Scholar
  21. Pinheiro JN, Freitas BM (2010) Efeitos dos pesticidas agrícolas sobre polinizadores e perspectivas de manejo para os agroecossistemas brasileiros. Oecol Aust 14:266–281CrossRefGoogle Scholar
  22. Ritz C, Streibig JC (2005) Bioassay analysis using R. J Stat Softw 12:1–22Google Scholar
  23. Roessink I, Steen JVD, Kasina M, Gikungu M, Nocelli RCF (2011) Is the European honeybee (Apis mellifera mellifera) a good representative for other pollinators species? In: SETAC Europe annual meeting, 21 May 2011, Milan, pp 3–35Google Scholar
  24. Rortais A, Arnold G, Halm MP, Touffet-Briens F (2005) Modes of honeybees exposure to systemic insecticides: estimated amounts of contaminated pollen and nectar consumed by different categories of bees. Apidologie 36:71–83CrossRefGoogle Scholar
  25. Stanley J, Chandrasekaran S, Preetha G, Kuttalam S (2010) Toxicity of diafenthiuron to honey bees in laboratory, semi-field and field conditions. Pest Manag Sci 66:505–510CrossRefGoogle Scholar
  26. R Development Core Team (2012) R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. ISBN 3-900051-07-0. http://www.R-project.org/. Accessed 20 June 2012
  27. Tingle CC, Rother JA, Dewhurst CF, Lauer S, King WJ (2003) Fipronil environmental fate, ecotoxicology and human health concerns. Rev Environ Contam Toxicol 176:1–66Google Scholar

Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Cynthia Renata Oliveira Jacob
    • 1
  • Hellen Maria Soares
    • 1
  • Stephan Malfitano Carvalho
    • 1
  • Roberta Cornélio Ferreira Nocelli
    • 2
  • Osmar Malaspina
    • 1
  1. 1.Departamento de Biologia, Centro de Estudos de Insetos SociaisUniv. Estadual Paulista (UNESP)Rio ClaroBrazil
  2. 2.Centro de Ciências AgráriasUniv. Federal de São CarlosArarasBrazil

Personalised recommendations