Lead Bone Toxicity in Growing Rats Exposed to Chronic Intermittent Hypoxia
- 212 Downloads
Lead chronic intoxication under hypoxic conditions revealed growth retardation in growing rats and damages on femoral and mandibular bones that predispose to fractures. These findings aimed us to investigate if bone material and geometric properties, bone mass in terms of histomorphometry or antioxidant capacity are also impaired in such experimental model. Combined treatments significantly reduced hemimandible cross sectional geometry and intrinsic stiffness (−16 % and −34 %); tibia and hemimandible bone volume (−45 % and −40 %) and growth plate cartilage thickness (−19 %). These results show a previously unreported toxic effect of lead on mandible however, longer studies should be necessary to evaluate if an adaptation of bone architecture to maintain structural properties may occur and if the oxidative stress can be identified as the primary contributory agent in the pathogenesis of lead poisoning.
KeywordsLead poisoning Intermittent hypoxia Bone biomechanics Bone histomorphometry Oxidative stress
The authors acknowledge the collaboration of physiology laboratory technicians Graciela M. Champin and Elsa Lingua, Department of Physiology, School of Dentistry, University of Buenos Aires. This work was supported by research grants from University of Buenos Aires (UBACyT 20020090200013).
- Conti MI, Terrizzi AR, Lee CM, Mandalunis PM, Bozzini C, Piñeiro AE, Martínez MP (2012) Effects of lead exposure on growth and bone biology in growing rats exposed to simulated high altitude. Bull Environ Contam Toxicol. doi: 10.1007/s00128-012-0602-2
- Ferretti JL (1997) Biomechanical properties of bone. From osteoporosis and bone densitometry. Springer, Berlin, pp 143–161Google Scholar
- Ferretti JL, Cointry GR, Capozza RF, Capiglioni R, Chiappe MA (2001) Analysis of biomechanical effects on bone and on the bone muscle interactions in small animal models. J Musculoskel Neuron Interact 1:263–274Google Scholar
- Haschek WM, Rousseaux CG, Wallig MA (2010) Bone toxicity. In: Fundamentals of toxicologic pathology, 2nd edn. Academic Press, imprint of Elsevier Inc., Typeset by Macmillan Publishing SolutionsGoogle Scholar
- Lyn Patrick ND (2006) Lead toxicity part II: the role of free radical damage and the use of antioxidants in the pathology and treatment of lead toxicity. Altern Med Rev 11(2):114–127Google Scholar
- Mosier HD (1969) Allometry of body weight and tail length in studies of catch-up growth in rats. Growth 33:319–330Google Scholar