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Imidacloprid Affects Pardosa Pseudoannulata Adults and Their Unexposed Offspring

Abstract

Imidacloprid is a nicotine-based, systemic, widely used insecticide. In order to investigate the effects of imidacloprid on the spider Pardosa pseudoannulata (Araneae: Lycosidae), specimens were exposed to different concentrations of imidacloprid (12.5, 25, 50, 100, 200 mg/L) by the dipping method. Surviving spiders were used to determine the fecundity, development time of unexposed offspring, predation, and the activities of detoxification enzymes. Significant reductions were observed in survival rate and fecundity of spiders exposed to imidacloprid. The development times of unexposed offspring (F1) were prolonged significantly with increased concentrations of imidacloprid. Spiders exposed to concentrations of imidacloprid above 25 mg/L showed significantly weaker predation on Drosophila melanogaster than the control group, but a low dose of imidacloprid (12.5 mg/L) increased predation ability. The activities of carboxyl esterase, acetyl cholinesterase, and the mixed-function oxidase were significantly inhibited by imidacloprid. With increasing concentrations of imidacloprid, the activities of all three kinds of enzymes were decreased significantly. These results suggest that imidacloprid can stimulate the performance of spiders (in low concentration) and has chronic toxicity to the spiders.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (31071895), the Foundation of Hubei Provincial Department of Education (Q20111001) and the Key Scientific and Technological Projects of Wuhan (201120722216-3). We thank Dr. Janet W. Reid (Biological consulting and editing services, JWR Associates, New York, USA) for emending the English of the manuscript.

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Correspondence to Yu Peng.

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Chen, X., Xiao, Y., Wu, L. et al. Imidacloprid Affects Pardosa Pseudoannulata Adults and Their Unexposed Offspring. Bull Environ Contam Toxicol 88, 654–658 (2012). https://doi.org/10.1007/s00128-012-0584-0

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Keywords

  • Pardosa pseudoannulata
  • Imidacloprid
  • Fecundity
  • Development times
  • Predation
  • Activities of detoxification enzymes