Sublethal Effects of Waterborne Herbicides in Tropical Freshwater Fish
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The study evaluated the sublethal effects of the herbicides glyphosate (Roundup) and diuron (Hexaron) and the mixture of them, used extremely in agriculture, through biomarkers in fish. The glutathione S-transferase activity increased (74%) and catalase activity decreased (37%) at the higher exposure concentration of Hexaron in comparison to the control group, suggesting an activation of this metabolism route. Membrane damage was observed at the higher exposure of Roundup and in the mixture group compared to the control group, which can be related to the nuclear alterations observed in these exposed groups. The cholinesterase activity was also inhibited (37%) in mixture group compared to the control group and no gill morphology damage was found. The results suggested a potential synergic effect in some analysed parameters.
KeywordsAstyanax sp. Bioassay Biomarkers Freshwater fish Herbicides
This research was supported in part by CNPq (Brazilian Agency for Science and Technology).
- Akaishi FM, Silva de Assis HC, Jakobi SCG, Eiras-stofella DR, Stjean S, Courtenay SC, Lima EF, Wagener AL, Scofield AL, Oliveira Ribeiro CA (2004) Morphological and neurotoxicological findings in tropical freshwater fish (Astyanax sp.) after waterborne and acute exposure to water soluble fraction (WSF) of crude oil. Arch Environ Contam Toxicol 46:244–253Google Scholar
- Grisolia CK (2002) A comparison between mouse and fish micronucleus test using cyclophosphamide, mitomycin C and various pesticides. Mutat Res 518:145–150Google Scholar
- Keen JH, Habig WH, Jakoby WB (1976) Mechanism for several activities of the glutathione S-transferases. J Biol Chem 251:6183–6188Google Scholar
- Langiano VC, Martinez CBR (2008) Toxicity and effects of a glyphosate-based herbicide on the Neotropical fish Prochilodus lineatus. Comp Biochem Physiol 147:222–231Google Scholar
- Souza MD, Boeira RC, Gomes MAF (2000) Adsorção e dessorção de diuron em solos tropicais. Pesticidas R Ecotoxicol e Meio Ambiente 10:113–124Google Scholar
- Teisseire H, Vernet G (2000) Is the “Diuron Effect” due to a herbicide strengthening of antioxidative defenses of Lemna minor? Comp Biochem Physiol 66:153–160Google Scholar
- World Health Organization (WHO) (1994) Glyphosate, international programme on chemical safety. Environ Health Criteria 159, Geneva, Switzerland, pp 110–112Google Scholar
- Zhonglin Z, Zhengjun S, Daoji C (1998) Evaluation on effect of hexazinone on eco-environment. Adv Environ Sci 6:11–20Google Scholar