Advertisement

Accumulation of Microcystins in Nile Tilapia, Oreochromis niloticus L., and Effects of a Complex Cyanobacterial Bloom on the Dietetic Quality of Muscles

  • Miroslava Palikova
  • Jan Mares
  • Radovan KoppEmail author
  • Jana Hlavkova
  • Stanislav Navratil
  • Ondrej Adamovsky
  • Lubomír Chmelar
  • Ludek Blaha
Article

Abstract

The aim of this study was to investigate the kinetics of accumulation and elimination of microcystins in the tissues of Nile tilapia (Oreochromis niloticus) and to evaluate the effect of cyanobacterial exposure on fish muscle quality (levels of total fat and ash, protein, dry matter and the composition of saturated, monounsaturated and polyunsaturated fatty acids). Fish were exposed for 28 days to a natural cyanobacterial bloom with total microcystin concentration around 1,200 μg g−1 biomass dry weight. The hepatopancreas accumulated microcystins up to 350 ng g−1 fresh weight, but concentrations in muscle were generally below the detection limit (2 ng g−1 fresh weight). Following the exposure, fish were moved to the clean water, but only minor microcystin removal from the hepatopancreas was observed during a 4 week depuration period. Exposures of tilapia to the complex cyanobacterial bloom had only minor and temporary impacts on the investigated parameters of dietetic quality.

Keywords

Fatty acids Cyanobacteria Bioaccumulation Fish 

Notes

Acknowledgments

The authors highly acknowledge comments of two anonymous referees that substantially helped to improve quality of the manuscript. This study was supported by the Ministry of Education, Youth and Sports of the Czech Republic “Veterinary aspects of food safety and quality” No. MSM6215712402, Research plan No. MSM6215648905 “Biological and technological aspects of sustainability of controlled ecosystems and their adaptability to climate change, and by the National Agency for Agricultural Research (grant No. QH 71015)”. Infrastructure is supported by a project CETOCOEN (no. CZ.1.05/2.1.00/01.0001) from the European Regional Development Fund.

References

  1. Adamovsky O, Kopp R, Hilscherova K, Babica P, Palikova M, Paskova V, Navratil S, Blaha L (2007) Microcystin kinetics (bioaccumulation, elimination) and biochemical responses in common carp and silver carp exposed to toxic cyanobacterial blooms. Environ Toxicol Chem 26:687–2693CrossRefGoogle Scholar
  2. Ahlgren G (1992) Fatty acid content and chemical composition of freshwater microalgae. J Phycol 28:37–50CrossRefGoogle Scholar
  3. APHA (1981) Standard methods for the examination of water and wastewater, 15th ed. American Public Health Association Inc., Washington, DCGoogle Scholar
  4. Blaha L, Marsalek B (2003) Contamination of drinking water in the Czech Republic by microcystins. Arch Hydrobiol 158:21–429CrossRefGoogle Scholar
  5. Blahova L, Babica P, Marsalkova E, Smutna M, Marsalek B, Blaha L (2007) Concentrations and seasonal trends of extracellular microcystins in freshwaters of the Czech Republic—results of the national monitoring program. CLEAN Soil Air Water 35:348–354CrossRefGoogle Scholar
  6. Deblois CP, Aranda-Rodrigues R, Gianic A, Bird DF (2008) Microcystin accumulation in liver and muscle of tilapia in two large Brazilian hydroelectric reservoirs. Toxicon 5:435–448CrossRefGoogle Scholar
  7. Folsch J, Lees M, Sloane-Stanley H (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226:497–509Google Scholar
  8. ISO 10260 (1992) Water quality. Measurement of biochemical parameters. Spectrometric determination of the chlorophyll-a concentration. Int. Org. Standard, 1st edn. GenevaGoogle Scholar
  9. Kohoutek J, Adamovsky O, Oravec M, Simek Z, Palikova M, Kopp R, Blaha L (2010) LC-MS analyses of microcystins in fish tissues overestimate toxin levels—critical comparison with LC-MS/MS. Anal Bionala Chem 398:1231–1237CrossRefGoogle Scholar
  10. Magalhaes VF, Soares RM, Azevedo SMFO (2001) Microcystin contamination in fish from the Jacarepagua Lagoon (Rio de Janeiro, Brazil): ecological implication and human health risk. Toxicon 39:1077–1085CrossRefGoogle Scholar
  11. Malbrouck C, Trausch G, Devos P, Kestemont P (2003) Hepatic accumulation and effects of microcystin-LR on juvenile goldfish Carassius auratus L. Comp Biochem Physiol C Toxicol Pharmacol 135:39–48Google Scholar
  12. Mares J, Palikova M, Kopp R, Navratil S, Pikula J (2009) Changes in the nutritional parameters of muscles of the common carp (Cyprinus carpio) and the silver carp (Hypophthalmichthys molitrix) following environmental exposure to cyanobacterial water bloom Aquacul Res 40:148–156Google Scholar
  13. Mohamed Z, Hussein A (2006) Depuration of microcystins in tilapia fish exposed to natural populations of toxic cyanobacteria: a laboratory study. Ecotox Environ Safe 63:424–429CrossRefGoogle Scholar
  14. Peng LA, Liu YM, Chen W, Liu LM, Kent M, Song LR (2010) Health risks associated with consumption of microcystin-contaminated fish and shellfish in three Chinese lakes: significance for freshwater aquacultures. Ecotox Environ Safe 73:1804–1811CrossRefGoogle Scholar
  15. Soares RM, Magalhaes VF, Azevedo SMFO (2004) Accumulation and depuration of microcystins (cyanobacteria hepatotoxins) in Tilapia rendalli (Cichlidae) under laboratory conditions. Aquat Toxicol 70:1–10CrossRefGoogle Scholar
  16. Tadesse Z, Boberg M, Sonesten L, Ahlgren G (2003) Effects of algal diets and temperature on the growth and fatty acid content of the cichlid fish Oreochromis niloticus L.—a laboratory study. Aquat Exil 37:169–182CrossRefGoogle Scholar
  17. Tricarico E, Bertocchi S, Brusconi S, Casalone E, Gherardi F, Giorgi G, Mastromei G, Parisi G (2008) Depuration of microcystin-LR from the red swamp crayfish Procambarus clarkii with assessment of its food quality. Aquaculture 285:90–95CrossRefGoogle Scholar
  18. WHO (1998) Guidelines for drinking water quality. World Health Organization, GenevaGoogle Scholar
  19. Yang H, Xie P, Xu J, Zheng L, Deng D, Zhou Q, Wu S (2006) Seasonal variation of microcystin concentration in Lake Chaohu, a shallow subtropical lake in the People’s Republic of China. Bull Environ Contam Toxicol 77:367–374CrossRefGoogle Scholar
  20. Zhang D, Xie P, Chen J (2010) Effects of temperature on the stability of microcystins in muscle of fish and its consequences for food safety. Bull Environ Contam Toxicol 84:202–207CrossRefGoogle Scholar
  21. Zhao M, Xie S, Zhu X, Yang Y, Gan N, Song L (2006) Effect of dietary cyanobacteria on growth and accumulation of microcystins in Nile tilapia (Oreochromis niloticus). Aquaculture 261:960–966CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Miroslava Palikova
    • 1
  • Jan Mares
    • 2
  • Radovan Kopp
    • 2
    • 5
    Email author
  • Jana Hlavkova
    • 2
  • Stanislav Navratil
    • 1
  • Ondrej Adamovsky
    • 3
  • Lubomír Chmelar
    • 4
  • Ludek Blaha
    • 3
    • 5
  1. 1.University of Veterinary and Pharmaceutical SciencesBrnoCzech Republic
  2. 2.Department of Fishery and HydrobiologyMendel UniversityBrnoCzech Republic
  3. 3.Faculty of Science, Research Centre for Toxic Compounds in the Environment, RECETOXMasaryk UniversityBrnoCzech Republic
  4. 4.MedistatBrnoCzech Republic
  5. 5.Institute of BotanyCzech Academy of SciencesBrnoCzech Republic

Personalised recommendations