Assessment of Genotoxic Effects of Boron on Wheat (Triticum aestivum L.) and Bean (Phaseolus vulgaris L.) by Using RAPD Analysis

Article

DOI: 10.1007/s00128-010-0031-z

Cite this article as:
Kekec, G., Sakcali, M.S. & Uzonur, I. Bull Environ Contam Toxicol (2010) 84: 759. doi:10.1007/s00128-010-0031-z

Abstract

In boron-rich soils of Turkey, boron tolerant wheat (Triticum aestivum L.) and sensitive bean (Phaseolus vulgaris L.) are most widely cultivated crops. In this study they have been studied to elucidate the probable genotoxic effects of boron by using RAPD analysis. During the study, root and stem lengths have been measured and inhibitory rates (%) of root growth have been found to be significant, starting from 10 (13%) and 5 ppm (19%) for wheat and bean, respectively, which is in strong correlation with the root DNA alterations; RAPD variations starting from 100 ppm for wheat and 25 ppm for bean. The preliminary findings encourage the use of these tools in investigation of genotoxic effects of boron on wheat, bean and the other crops.

Keywords

RAPD-PCR Genotoxicity Boron Wheat Bean 

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Department of BiologyFatih UniversityIstanbulTurkey

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