Diazinon Determination Using High Performance Liquid Chromatography: A Comparison of the ENVI-Carb Column with the Immunoaffinity Column for the Pretreatment of Water and Soil Samples

Article

Abstract

An immunoaffinity chromatography column was developed to remove diazinon from water and soil samples. In this paper, two types of absorbent columns, the immunoaffinity chromatography column and the ENVI-Carb column, were compared. To accomplish this, each of these columns was used to treat water and soil samples that had been spiked with diazinon at concentrations of 2.5 or 5 ng/mL (or ng/g). High performance liquid chromatography was then used to analyze the treated samples. The ENVI-Carb column recovered 87.99%–95.95% of the diazinon from water and soil with CVs of 5.08%–8.06%. The recoveries observed when the immunoaffinity chromatography column was used were slightly lower (52.61%–81.58%); however, it effectively clean up the soil samples.

Keywords

Immunoaffinity Solid phase extraction Diazinon Comparison 

References

  1. Bavcon M, Trebše P, Zupančič-Kralj L (2003) Investigations of the determination and transformations of diazinon and malathion under environmental conditions using gas chromatography coupled with a flame ionisation detector. Chemosphere 50:595–601CrossRefGoogle Scholar
  2. Cichna M, Markl P, Knopp D, Niessner R (2001) On-line coupling of sol–gel-generated immunoaffinity columns with high-performance liquid chromatography. J Chromatogr A 919:51–58CrossRefGoogle Scholar
  3. Degelmann P, Egger S, Jürling H, Müller J, Niessner R, Knopp D (2006) Determination of sulfonylurea herbicides in water and food samples using sol–gel glass-based immunoaffinity extraction and liquid chromatography-ultraviolet/diode array detection or liquid chromatography-tandem mass spectrometry. J Agric Food Chem 54:2003–2011CrossRefGoogle Scholar
  4. Godfrey MAJ (1998) Immunoaffinity extraction in veterinary residue analysis – a regulatory viewpoint. Analyst 123:2501–2506CrossRefGoogle Scholar
  5. Hernández F, Sancho JV, Pozo OJ (2005) Critical review of the application of liquid chromatography/mass spectrometry to the determination of pesticide residues in biological samples. Anal Bioanal Chem 382:934–946CrossRefGoogle Scholar
  6. Herrera A, Pérez-Arquillué C, Conchello P, Bayarri S, Lázaro R, Yagüe C, Ariño A (2005) Determination of pesticides and PCBs in honey by solid-phase extraction cleanup followed by gas chromatography with electron-capture and nitrogen-phosphorus detection. Anal Bioanal Chem 381:695–701CrossRefGoogle Scholar
  7. Kaware M, Bronshtein A, Safi J, Van Emon JM, Chuang JC, Hock B, Kramer K, Altstein M (2006) Enzyme-linked immunosorbent assay and sol–gel based immunoaffinity purification of the pyrethroid bioallethrin in food and environmental samples. J Agric Food Chem 54:6482–6492CrossRefGoogle Scholar
  8. Kumazawa T, Suzuki O (2000) Separation methods for amino group-possessing pesticides in biological samples. J Chromatogr-Biomed 747:241–254CrossRefGoogle Scholar
  9. Muggleton NG, Smith AJ, Scott EAM, Wilson SJ, Pearce PC (2005) A long-term study of the effects of diazinon on sleep, the electrocorticogram and cognitive behaviour in common marmosets. J Psychopharmacol 19:455–466CrossRefGoogle Scholar
  10. Pharmacia LKB Biotechnology (1995) Affinity chromatography: principles and methods. Pharmacia, SwedenGoogle Scholar
  11. Sullivan JJ, Goh KS (2000) Evaluation and validation of a commercial ELISA for diazinon in surface waters. J Agric Food Chem 48:4071–4078CrossRefGoogle Scholar
  12. Thomas SD, Li QX (2000) Immunoaffinity chromatography for analysis of polycyclic aromatic hydrocarbons in corals. Environ Sci Technol 34:2649–2654CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Jianshe Tang
    • 1
  • Min Zhang
    • 1
  • Guohua Cheng
    • 1
  • Yitong Lu
    • 1
  1. 1.Resource and Environmental Science Department, Agricultural and Biology SchoolShanghai Jiao Tong UniversityShanghaiPeople’s Republic of China

Personalised recommendations