Four-year-old seedlings of Ginkgo biloba were exposed to elevated O3, elevated CO2 and elevated O3 plus elevated CO2 in open-top chambers (OTCs) to study the responses of antioxidant system in Ginkgo biloba leaves. No significant changes in reactive oxygen production and scavenging systems were detected in seedlings exposed to high CO2. Significant increase in H2O2 and MDA content were induced by elevated O3. The ascorbate content and antioxidative enzymes activity were increased significantly by exposure to high O3 as well. But the promoted ability in scavenging did not prevent the increase in H2O2 content and cell membrane lipid peroxidation. The increase was mitigated by high CO2 in the combined exposure, but the effect was hardly significant.
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Aebi H, Lester P (1984) Catalase in vitro. Method Enzymol 105:121–126. doi:10.1016/S0076-6879(84)05016-3
Chernikova T, Robinson JM, Lee EH, Mulchi CL (2000) Ozone tolerance and antioxidant enzyme activity in soybean cultivars. Photosynth Res 64:15–26. doi:10.1023/A:1026500911237
He XY, Ruan YN, Chen W, Lu T (2006) Responses of anti-oxidative system in leaves of Ginkgo biloba to elevated ozone concentration in urban area. Bot Stud 47:409–416
He XY, Fu SL, Chen W, Zhao TH, Xu S, Tuba Z (2007) Changes in effects of ozone exposure on growth, photosynthesis, and respiration of Ginkgo biloba in Shenyang urban area. Photosynthetica 45:555–561. doi:10.1007/s11099-007-0095-0
Hodges DM, DeLong JM, Forney CF, Prange RK (1999) Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta 207:604–611. doi:10.1007/s004250050524
Jin YH, Tao DL, Hao ZQ, Ye J, Du YJ, Liu HL, Zhou YB (2003) Environmental stresses and redox status of ascorbate. Acta Bot Sin 45:795–801
Karnosky DF (2003) Impacts of elevated atmospheric CO2 on forest trees and forest ecosystems: knowledge gaps. Environ Int 29:161–169. doi:10.1016/S0160-4120(02)00159-9
Krivosheeva A, Tao DL, Ottander C, Wingsle G, Dube SL, Oquist G (1996) Cold acclimation and photoinhibition of photosynthesis in Scots pine. Planta 200:296–305
Kull O, Sober A, Coleman MD, Dickson RE, Isebrands JG, Gagnon Z, Karnosky DF (1996) Photosynthetic responses of aspen clones to simultaneous exposures of ozone and CO2. Can J For Res 26:639–648
Liu HL, Du YJ, Yang ZY (1994) High effective liquid chromatographic analysis of vitamin C in Pinus koraiensis and Picea koraiensis leaves. Chin J Appl Ecol 5:442–443
Mukherjee SP, Choudhuri MA (1983) Implications of water stress-induced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings. Physiol Plant 58:166–170. doi:10.1111/j.1399-3054.1983.tb04162.x
Polle A, Pfirrmann T, Chakrabarti S, Rennenberg H (1993) The effects of enhanced ozone and enhanced carbon-dioxide concentrations on biomass, pigments and antioxidative enzymes in spruce needles. Plant Cell Environ 16:311–316. doi:10.1111/j.1365-3040.1993.tb00874.x
Schwanz P, Haberle KH, Polle A (1996) Interactive effects of elevated CO2, ozone and drought stress on the activities of antioxidative enzymes in needles of Norway spruce trees grown with luxurious N-supply. J Plant Physiol 148:351–355
Song SQ, Berjak P, Pammenter N (2004) Desiccation sensitivity of Trichilia dregeana axes and antioxidant role of ascorbic acid. Acta Bot Sin 46:803–810
Tausz M, Olszyk DM, Monschein S, Tingey DT (2004) Combined effects of CO2 and O3 on antioxidative and photoprotective defense systems in needles of ponderosa pine. Biol Plant 48:543–548. doi:10.1023/B:BIOP.0000047150.82053.e9
Wang XK, Manning W, Feng ZW, Zhu YG (2007) Ground-level ozone in China: distribution and effects on crop yields. Environ Pollut 47:394–400. doi:10.1016/j.envpol.2006.05.006
This work was funded by the National Natural Science Foundation of China Important Project 90411019, the Foundation of Knowledge Innovation Program of Chinese Academy of Sciences kzcx3-sw-43.
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Lu, T., He, X., Chen, W. et al. Effects of Elevated O3 and/or Elevated CO2 on Lipid Peroxidation and Antioxidant Systems in Ginkgo biloba Leaves. Bull Environ Contam Toxicol 83, 92–96 (2009). https://doi.org/10.1007/s00128-009-9719-3
- Elevated O3 and CO2
- Antioxidant system