Rapid Method for Multi-residue Determination of Avermectins in Bovine Liver Using High-performance Liquid Chromatography with Fluorescence Detection

Article
First Online:
Received:
Accepted:

Abstract

A simple method using high-performance liquid chromatography and fluorescence detection is presented for the simultaneous determination of the antiparasitic agents avermectins (abamectin, ivermectin and doramectin) in bovine liver. Samples were extracted using acetonitrile and cleaned up on an alumina SPE column and derivatized with trifluoroacetic anhydride. The derivatives were detected using fluorescence detector set at 365 nm excitation and 475 nm emission wavelengths. As the results, the limit of quantification for three avermectins was about 1 μg/kg. Inter-assay showed the mean recoveries of avermectins in bovine liver ranged from 72% to 81%, with RSD less than 9.9%.

Keywords

Avermectins Residue Liver Fluorescence 
This is a preview of subscription content, log in to check access.

Notes

Acknowledgements

Funding supports from Natural Science Foundation of China (20621703) and the Foundation of Ministry of Science & Technology of China (2002BA906A75) are gratefully acknowledged.

References

  1. Regulation No. 235 (2002) Ministry of Agriculture, People’s Republic of China, 24 Dec, Maximum residue limit in food of animal originGoogle Scholar
  2. Council Regulation (EEC) No. 2377/90 of 26 June 1990 (1990) Maximum residue limits of veterinary medicinal products in foodstuffs of animal origin. Off J Eur Commun L224:1Google Scholar
  3. David AD (2007) Positive and negative electrospray LC-MS/MS methods for quantitation of the antiparasitic endectocide drugs, abamectin, doramectin, emamectin, eprinomectin, ivermectin, moxidectin and selamectin in milk. J Chromatogr B 850:134–146. doi: 10.1016/j.jchromb.2006.11.014 CrossRefGoogle Scholar
  4. Pierre M, Shim Jung-Sook K, Pivnichny James V (1990) Liquid chromatographic determination of ivermectin in animal plasma with trifluoroacetic anhydride and N-methylimidazole as the derivatization reagent. J Pharm Biomed Anal 8:507–511. doi: 10.1016/0731-7085(90)80060-3 CrossRefGoogle Scholar
  5. Prieto JG, Merino G, Pulido MM, Estevez E, Molina AJ, Vila L, Alvarez AI (2003) Improved LC method to determine ivermectin in plasma. J Pharm Biomed Anal 31:639–645. doi: 10.1016/S0731-7085(02)00720-3 CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  1. 1.State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental SciencesChinese Academy of ScienceHaidian District, BeijingPeople’s Republic of China
  2. 2.Quality Control and Inspection Center for Animal Products, Ministry of AgricultureChina Animal Disease Control CenterChaoyang District, BeijingPeople’s Republic of China
  3. 3.Beijing Research Institute of Chemical Engineering and MetallurgyTongzhou District, BeijingPeople’s Republic of China

Personalised recommendations