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Effects of Dietary Benzo[a]pyrene on Growth and Hematological Parameters in Juvenile Rockfish, Sebastes schlegeli (Hilgendorf)

  • Seong Gil KimEmail author
  • Dae Kuk Park
  • Suck Woo Jang
  • Jae Seong Lee
  • Seong Soo Kim
  • Mi Hee Chung
Article

Abstract

Experiments were conducted to evaluate the effect of dietary benzo[a]pyrene (B[a]P) on the growth and hematological parameters of rockfish, Sebastes schlegeli, after they were fed with subchronic dietary B[a]P for 30 days. The weight growth rate of the rockfish was significantly different from that of the control group at dietary B[a]P concentration of 1.5 and 2.0 mg/kg. Significant reduction in red blood cells, hematocrit, and hemoglobin were observed during 30 days of exposure to the highest concentration. Over 30 days, serum AST increased at a B[a]P concentration of 1.5 mg/kg and LDH increased at a B[a]P concentration of 1.0 mg/kg concentration. However, no changes were observed in ALT, total protein, magnesium or calcium.

Keywords

Dietary benzo[a]pyrene Sebastes schlegeli Growth Hematological parameter 

Notes

Acknowledgement

This work is funded by a grant from the National Fisheries Research and Development Institute (RP-2008-ME-032) of Korea.

References

  1. Bálint T, Ferenczy J, Kátai F, Kiss I, Kráczer L, Kufcsák O, Láng G, Polyhos C, Szabó I, Szegletes T, Nemcsók J (1997) Similarities, differences between the massive eel (Anguilla anguilla L.) devastations that occurred in Lake Balaton in 1991 and 1995. Ecotoxicol Environ Saf 37:17–23. doi: 10.1006/eesa.1996.1509 CrossRefGoogle Scholar
  2. Boleas S, Fernandez C, Beyer J, Tarazona JV, Golsøyr A (1998) Accumulation and effects of benzo(a)pyrene on cytochrome P450 1A in waterborne exposed and intraperitoneal injected juvenile turbot (Scophthalmus maximus). Mar Environ Res 46:17–20. doi: 10.1016/S0141-1136(98)00022-1 CrossRefGoogle Scholar
  3. German Society for Clinical Chemistry (1972) Recommendations of the german association for clinical chemistry (DGKC). J Clinic Chem Clinic Biochem 10:182–193Google Scholar
  4. Gill TS, Epple A (1993) Stress-related changes in the hematological profile of the American eel (Anguilla rostrata). Ecotoxicol Environ Saf 25:227–235. doi: 10.1006/eesa.1993.1021 CrossRefGoogle Scholar
  5. Haux C, Larsson A (1984) Long-term sublethal physiological effects in rainbow trout, Salmo gairdneri, during exposure to cadmium and after subsequent recovery. Aquatic Toxicol 5:129–142. doi: 10.1016/0166-445X(84)90004-3 CrossRefGoogle Scholar
  6. Heintz RA, Rice SD, Wertheimer AC, Bradshaw RF, Thrower FP, Joyce JE, Short JW (2000) Delayed effects on growth and marine survival of pink salmon Oncorhynchus gorbuscha after exposure to crude oil during embryonic development. Mar Ecol Prog Ser 208:205–216. doi: 10.3354/meps208205 CrossRefGoogle Scholar
  7. Holbrook DJ (1994) Effects of toxicants on nucleic acid and protein metabolism. In: Hodgson E, Levi PE (eds) Introduction to biochemical toxicology. Appleton & Langer, Conenecticut, pp 367–379Google Scholar
  8. Jee JH, Kim SG, Kang JC (2004) Effects of phenanthrene on growth and basic physiological functions of the olive flounder, Paralichthys olivaceus. J Exp Mar Biol Ecol 304:123–136. doi: 10.1016/j.jembe.2003.12.001 CrossRefGoogle Scholar
  9. Kwon JY, Chang YJ (1996) Effects of ammonia concentration on histological and physiological status in black seabream Acanthopagrus schlegeli. J Korean Fish Soc 29:828–836Google Scholar
  10. Lemaire P, Drai P, Mathieu A, Lemaire S, Carriere S, Giudicelli J, Lafaurie M (1991) Changes with different diets in plasma enzymes (GOT, GPT, LDH, ALP) and plasma lipids (cholesterol, triglycerides) of sea-bass (Dicentrachus labrax). Aquaculture 93:63–75. doi: 10.1016/0044-8486(91)90205-L CrossRefGoogle Scholar
  11. Long SM, Ryder KJ, Holdwaya DA (2003) The use of respiratory enzymes as biomarkers of petroleum hydrocarbon exposure in Mytilus edulis planulatus. Ecotoxicol Environ Saf 55:261–270. doi: 10.1016/S0147-6513(02)00137-9 CrossRefGoogle Scholar
  12. Moles A, Babcock MM, Rice SD (1987) Effects of oil exposure on pink salmon, Oncorhynchus gorbuscha, alevins in a simulated intertidal environment. Mar Environ Res 21:49–58. doi: 10.1016/0141-1136(87)90073-0 CrossRefGoogle Scholar
  13. Nelson DL, Cox MM (2000) Lehninger Principles of Biochemistry, 3rd ed. Worth Publishers, New YorkGoogle Scholar
  14. Reitman S, Frankel S (1957) A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am J Clinic Pathol 28:56–63Google Scholar
  15. Schwaiger J, Spieser OH, Bauer C, Ferling H, Mallow U, Kalbfus W, Negele RD (2000) Chronic toxicity of nonylphenol and ethinylestradiol: haematological and histopathological effects in juvenile common carp (Cyprinus carpio). Aquatic Toxicol 51:69–78. doi: 10.1016/S0166-445X(00)00098-9 CrossRefGoogle Scholar
  16. Vaglio A, Landriscina C (1999) Changes in liver enzyme activity in the teleost Sparus aurata in response to cadmium intoxication. Ecotoxicol Environ Saf 43:111–116. doi: 10.1006/eesa.1999.1778 CrossRefGoogle Scholar
  17. van der Oost R, Vindimianb E, van den Brink PJ, Satumalay K, Heida H, Vermeulend NPE (1997) Biomonitoring aquatic pollution with feral eel (Anguilla anguilla). III. Statistical analyses of relationships between contaminant exposure and biomarkers. Aquatic Toxicol 39:45–75. doi: 10.1016/S0166-445X(96)00851-X CrossRefGoogle Scholar
  18. Walker GW, Livingstone DR (1992) Persistent pollutants in marine ecosystems. Pergamon Press, OxfordGoogle Scholar
  19. Wedemeyer GA, Yasutake WT (1977) Clinical methods for the assessment of the effects of environmental stress on fish health. U.S. Tech Pap USFWS 89:1–18Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Seong Gil Kim
    • 1
    Email author
  • Dae Kuk Park
    • 1
  • Suck Woo Jang
    • 1
  • Jae Seong Lee
    • 1
  • Seong Soo Kim
    • 1
  • Mi Hee Chung
    • 2
  1. 1.Marine Environmental Management TeamNational Fisheries Research and Development InstituteBusanSouth Korea
  2. 2.Institute of Environmental StudiesPusan National UniversityBusanSouth Korea

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