Effect of the Herbicide Roundup® on Perkinsus olseni in vitro Proliferation and in vivo Survival when Infecting a Permissive Host, the Clam Ruditapes decussatus
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Coastal habitats are increasingly being exposed to herbicide contamination from urban and agricultural catchments. Data on its toxicity on aquatic ecosystems, especially those based on sediment, are relatively scarce. This study aimed at investigating whether the susceptibility of an aquatic filter-feeding organism, the carpet-shell clam (Ruditapes decussatus) to the parasite Perkinsus olseni was influenced by the herbicide Roundup® and its active ingredient glyphosate. The effect of Roundup® and glyphosate on P. olseni in vitro proliferation was also evaluated and appeared to confirm the higher toxicity of Roundup when compared with technical grade glyphosate.
KeywordsPerkinsus olseni Carpet-shell clam Glyphosate Roundup®
This work was funded in part by grants POCTI/BIO/12143/1998 and POCTI/MAR/15308/1999 from the Portuguese Science and Technology Foundation. R. B. Leite is a recipient of PhD fellowships from the Portuguese Science and Technology Foundation, reference SFRH/BD/30112/2006.
- Abdelghani AA, Tchounwou PB, Anderson AC, Sujono H, Heyer LR, Monkiedje A (1997) Toxicity evaluation of single and chemical mixtures of Roundup, Garlon-3A, 2,4-D, and syndets surfactant to channel catfish (Ictalurus punctatus), bluegill sunfish (Lepomis microchirus) and crawfish (Procambarus spp.). Environ Toxicol Water Qual 12:237–244CrossRefGoogle Scholar
- Anderson RS, Burreson EM, Unger MA (1995) The effects of environmental contaminants on the progression of Perkinsus marinus infection in the eastern oyster. In: Chesapeake Bay environmental effect studies. Toxics research program 1994 workshop report. Solomons, Md. Virginia Sea Grant College Program, pp 150–155Google Scholar
- Bushek D, Allen SK Jr, Alcox KA, Gustafson RG, Ford SE (1997) Response of Crassostrea virginica to in vitro cultured Perkinsus marinus: preliminary comparisons of three inoculation methods. J Shellfish Res 16:479–485Google Scholar
- Fisher WS, Oliver LM, Sutton EB, Manning CS, Walker WW (1995) Exposure of eastern oysters to tributyltin increases the severity of Perkinsus marinus disease. J Shellfish Res 14:265–266Google Scholar
- Ruano F, Cachola R (1986) Outbreak of a severe epizootic of Perkinsus marinus (Levin-78) at Ria de Faro clam’s culture beds. 2nd Int Colloq Mar Aquac 41–42Google Scholar
- Worthing CR (1987) The pesticide manual. British Crop Protection Council, Worcestershire, p 1077Google Scholar