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Cytogenetic Biomonitoring of the Mzamza Population Exposed to Untreated Wastewaters

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Abstract

A cytogenetic biomonitoring study was performed on people from the Mzamza community near Settat, Morocco. These subjects live in and near the Bou Moussa valley where wastewaters from a great number of industries are discharged without any treatment. This wastewater is used as a source of drinking water for their cattle and irrigation of their land. The Mzamza population is therefore presumably exposed to continuous low doses of different kinds of pollutants. Our study demonstrated significant increases in micronucleated white blood cells indicating a considerable genetic risk in these subjects.

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References

  1. Albertini RJ, Anderson D, Douglas GR, Hagmar L, Hemminki K, Merlo F, Natarajan AT, Norpa H, Shuker DEG, Tice R, Waters MD, Aitio A (2000) IPCS Guidelines for the monitoring of genoxic effects of carcinogens in humans. Mutat Res 463:111–172

  2. Anderson D (1999) Factors contributing to biomarkers responses in exposed workers. Mutat Res 428:197–202

  3. Bonassi S, Abbondandolo A, Camuri L, Dal Pra De Ferrari M, Degrassi F et al (1995) Are chromosome aberrations in circulating lymphocytes predictive of a future cancer onset in humans? Preliminary results of an Italian cohort study. Cancer Genet. Cytogenet 79:133–135

  4. Bonassi S, Znaor A, Ceppi M, Lando C, Chang WP, Holland N, Kirsch-Volders M, Zeiger E, Ban S, Barale R, Bigatti MP, Bolognesi C, Cebulska-Wasilewska A, Fabianova E, Fucic A, Hagmar L, Joksic G, Martelli A, Migliore L, Mirkova E, Scarfi MR, Zijno A, Norppa H, Fenech M (2007) An increased micronucleus frequency in peripheral blood lymphocytes predicts the risk of cancer in humans. Carcinogenesis 28:625–631

  5. Carrano AV, Natarajan AT (1988) ICPEMC: International Commission for Protection against Environmental Mutagens and Carcinogens; publication n°14 “considerations for population monitoring using cytogenetic techniques”. Mutat Res 204:379–406

  6. EHC (2001) Biomarkers. In risk assessment: validity and validation, environmental health monograph 222, IPCS-INCHEM

  7. Fenech M (2000) The in vitro micronucleus technique. Mutat Res 455:81–95

  8. Hagmar L, Brøgger A, Hansteen I-L, Heim S, Högstedt B, Knudsen L, Lambert B, Linnainmaa K, Mitelman F, Nordenson I, Reuterwall C, Salomaa S, Skerfving S, Sorsa M (1994) Cancer risk in humans predicted by increased levels of chromosomal aberrations in lymphocytes: Nordic Study Group on the health risk of chromosome damage. Cancer Res 54:2919–2922

  9. Hung HT, Gerber GB, Léonard ED, Crutzen-Fayt MC, Richard F, Léonard A (1995) Utilisation du test micronoyaux comme dosimètre biologique en cas d’exposition homogène ou non homogène aux radiations ionisantes C R Seances Soc Biol 189:1137–1142

  10. IAEA (1986) International Atomic Energy Agency. Biological dosimetry: chromosomal aberration analysis for dose-assessment, Vienna, ISBN 92-0-125086-X

  11. Jemali A, Kefati A (2002) Forum de la gestion de la demande en eau “réutilisation des eaux usées, cas du Maroc”. Ministère de l’agriculture, Rabat, Maroc, Mars 2002

  12. Kholtei S, Bouzidi A, Bonini M, Fekhaoui M, Sbai K, Anane R, Creppy EE (2003) Contamination des eaux souterraines de la plaine de Berréchid dans la région de Chaouia au Maroc par des métaux présents dans les eaux usées: effet de la pluviométrie. Vecteur Environ 36:68–80

  13. Klemans W, Vleminckx C, Schriewer L, Joris I, Maes A, Ottogali M, Pays A, Planard C, Rigaux G, Ros Y, Vande Rivière M, Vandenvelde J, Verschaeve L, Deplaen P, Lakhanisky Th (1995) Cytogenetic biomonitoring of a population of children allegedly exposed to environmental pollutants, phase 2: results of a three-year longitudinal study. Mutat Res. 342:147–156

  14. Laamari A, El Kettani S, Bouzidi A, and Tanji A (2004) Evaluation de l’impact de l’utilisation des eaux usées en agriculture sur l’écocystème et sur la santé humaine de la communauté Mzamza; rapport d’activité (Septembre 2002–Avril 2004), projet INRA/CRDI N°100771−004

  15. Léonard A, Léonard ED, Decat G, Hilali A (1998) Surveillance biologique, par analyse des anomalies chromosomiques des personnes ayant pu être exposées aux radiations ionisants. Ann Assoc Belge Radioprot 23:113–123

  16. Martinez V, Creus A, Venegas W, Arroyo A, Beck JP, Gebel TW, Surrallés J, Marcos R (2004) Evaluation of micronucleus induction in a Chilean population environmentally exposed to arsenic. Mutat Res 564:65–74

  17. Meftah El Kadmiri I, Glouib K, Verschaeve L, Hilali A (2006) Cytogenetic monitoring of domestic mammals exposed to wastewaters from the localities of Dladla and Boukallou near Settat, Morocco. Environ Int 32:690–696

  18. Mitelman F (1994) chromosomes, genes and cancer (editorial). CA Cancer J Clin 44:133–135

  19. Obe G, Beek G (1982) The human leukocyte system. In: de Serres FJ, Hollaender A (eds) Chemical mutagens and methods for their detection, vol.7. Plenum, New York, pp 337–400

  20. Obe G, Beek G (1984) Human peripheral lymphocytes in mutation research. In: Obe G (ed) Mutation in man. Springer, Berlin, pp 177–197

  21. Rew DA (1994) Significance of aneuploidy. Brit J Surg 81:1416–1422

  22. Sang N, Li G.K (2004) Genotoxicity of municipal landfill leachate on root tips of Vicia fava. Mutat Res 560:159–165

  23. Sang N, Li GK (2005) chromosomal aberration induced in mouse bone marrow cells by municipal landfill leachate. Environ Toxicol Pharmacol 20:219–224

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Acknowledgements

This work received the financial support of the Research Center for the International Development and Ford Foundation (IDRC/Ford programs Ecosanté N° 100771-0004).

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Correspondence to A. Hilali.

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Glouib, K., Hilali, A., Kettani, S.E. et al. Cytogenetic Biomonitoring of the Mzamza Population Exposed to Untreated Wastewaters. Bull Environ Contam Toxicol 79, 231–236 (2007). https://doi.org/10.1007/s00128-007-9180-0

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Keywords

  • Cytogenetic biomonitoring
  • Micronucleus test
  • Mitotic index
  • Proliferation index
  • Mzamza population
  • Wastewater