Diabetologia

, Volume 44, Issue 9, pp 1189–1196

The LEW.1AR1/Ztm-iddm rat: a new model of spontaneous insulin-dependent diabetes mellitus

  • S. Lenzen
  • M. Tiedge
  • M. Elsner
  • S. Lortz
  • H. Weiss
  • A. Jörns
  • G. Klöppel
  • D. Wedekind
  • C. -M. Prokop
  • H. J. Hedrich

Abstract.

Aims/hypothesis:

We describe a new Type I (insulin-dependent) diabetes mellitus rat model (LEW.1AR1/Ztm-iddm) which arose through a spontaneous mutation in a congenic Lewis rat strain with a defined MHC haplotype (RT1.Aa B/Du Cu).

Methods:

The development of diabetes was characterised using biochemical, immunological and morphological methods.

Results:

Diabetes appeared in the rats with an incidence of 20 % without major sex preference at 58 ± 2 days. The disease was characterised by hyperglycaemia, glycosuria, ketonuria and polyuria. In peripheral blood, the proportion of T lymphocytes was in the normal range expressing the RT6.1 differentiation antigen. Islets were heavily infiltrated with B and T lymphocytes, macrophages and NK cells with beta cells rapidly destroyed through apoptosis in areas of insulitis.

Conclusion/interpretation:

This Type I diabetic rat develops a spontaneous insulin-dependent autoimmune diabetes through beta cell apoptosis. It could prove to be a valuable new animal model for clarifying the mechanisms involved in the development of autoimmune diabetes. [Diabetologia (2001) 44: 1189–1196]

Keywords Type I diabetes mellitus, animal model, rat, apoptosis. 

Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  • S. Lenzen
    • 1
  • M. Tiedge
    • 1
  • M. Elsner
    • 1
  • S. Lortz
    • 1
  • H. Weiss
    • 1
  • A. Jörns
    • 2
  • G. Klöppel
    • 3
  • D. Wedekind
    • 4
  • C. -M. Prokop
    • 4
  • H. J. Hedrich
    • 4
  1. 1.Institute of Clinical Biochemistry, Hannover Medical School, Hannover, GermanyDE
  2. 2.Centre of Anatomy, Hannover Medical School, Hannover, GermanyDE
  3. 3.Institute of Pathology, University of Kiel, Kiel, GermanyDE
  4. 4.Institute for Laboratory Animal Science, Hannover Medical School, Hannover, GermanyDE

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