Diabetologia

, Volume 43, Issue 5, pp 583–588

Short-term treatment with GLP-1 increases pulsatile insulin secretion in Type II diabetes with no effect on orderliness

  • C. B. Juhl
  • O. Schmitz
  • S. Pincus
  • J. J. Holst
  • J. Veldhuis
  • N. Pørksen
Articles

Abstract

Aims/hypothesis. The enteric incretin hormone, glucagon-like peptide-1 (GLP-1), is a potent insulin secretagogue in healthy humans and patients with Type II (non-insulin-dependent) diabetes mellitus. In this study we assessed the impact of short-term GLP-1 infusion on pulsatile insulin secretion in Type II diabetic patients. Methods. Type II diabetic patients (n = 8) were studied in a randomised cross-over design. Plasma insulin concentration time series were obtained during basal conditions and during infusion with saline or GLP-1 (1.2 pmol/l · kg–1· min–1) on 2 separate days. Plasma glucose was clamped at the initial concentration by a variable glucose infusion. Serum insulin concentration time series were evaluated by deconvolution analysis, autocorrelation analysis, spectral analysis and approximate entropy. Results. Serum insulin concentrations increased by approximately 100 % during GLP-1 infusion. Pulsatile insulin secretion was increased as measured by secretory burst mass (19.3 ± 3.8 vs 53.0 ± 10.7 pmol/l/pulse, p = 0.02) and secretory burst amplitude (7.7 ± 1.5 vs 21.1 ± 4.3 pmol/l/min, p = 0.02). A similar increase in basal insulin secretion was observed (3.6 ± 0.9 vs 10.2 ± 2.2 pmol/l/min, p = 0.004) with no changes in the fraction of insulin delivered in pulses (0.50 ± 0.06 vs 0.49 ± 0.02, p = 0.84). Regularity of secretion was unchanged as measured by spectral analysis (normalised spectral power: 5.9 ± 0.6 vs 6.3 ± 0.8, p = 0.86), autocorrelation analysis (autocorrelation coefficient: 0.19 ± 0.04 vs 0.18 ± 0.05, p = 0.66) and the approximate entropy statistic (1.48 ± 0.02 vs 1.51 ± 0.02, p = 0.86). Conclusion/interpretation. Short-term stimulation with GLP-1 jointly increases pulsatile and basal insulin secretion, maintaining but not improving system regularity in Type II diabetic patients. [Diabetologia (2000) 43: 583–588]

Keywords GLP-1 Insulin pulsatility insulin secretion time series Type II diabetes human. 

Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • C. B. Juhl
    • 1
  • O. Schmitz
    • 1
  • S. Pincus
    • 2
  • J. J. Holst
    • 3
  • J. Veldhuis
    • 4
  • N. Pørksen
    • 1
  1. 1.Department of Medicine M (Endocrinology and Diabetes), University Hospital of Aarhus, Aarhus, DenmarkDK
  2. 2.Guilford, Connecticut, USAUS
  3. 3.Department of Medical Physiology, University of Copenhagen, DenmarkDK
  4. 4.Endocrinology Division, General Clinical Research Center, University of Virginia, Charlottesville, Virginia, USAUS

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