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Diabetologia

, Volume 42, Issue 9, pp 1071–1079 | Cite as

Changes in glucose transport and protein kinase Cβ2 in rat skeletal muscle induced by hyperglycaemia

  • Y. Kawano
  • J. Rincon
  • A. Soler
  • J. W. Ryder
  • L. A. Nolte
  • J. R. Zierath
  • H. Wallberg-Henriksson
Short communication

Abstract

Aims/hypothesis. We have previously reported that hyperglycaemia activates glucose transport in skeletal muscle by a Ca2+-dependent pathway, which is distinct from the insulin-signalling pathway. The aim of this study was to explain the signalling mechanism by which hyperglycaemia autoregulates glucose transport in skeletal muscle. Methods. Isolated rat soleus muscle was incubated in the presence of various concentrations of glucose or 3-O-methylglucose and protein kinase C and phospholipase C inhibitors. Glucose transport activity, cell surface glucose transporter 1 and glucose transporter 4 content and protein kinase C translocation was determined. Results. High concentrations of 3-O-methylglucose led to a concentration-dependent increase in [3H]-3-O-methylglucose transport in soleus muscle. Dantrolene, an inhibitor of Ca2+ released from the sarcoplasmic reticulum, decreased the Vmax and the Km of the concentration-response curve. Protein kinase C inhibitors (H-7 and GF109203X) inhibited the stimulatory effect of high glucose concentrations on hexose transport, whereas glucose transport stimulated by insulin was unchanged. Incubation of muscle with glucose (25 mmol/l) and 3-O-methylglucose (25 mmol/l) led to a three fold gain in protein kinase Cβ 2 in the total membrane fraction, whereas membrane content of protein kinase Cα, β 1, δ, ɛ and ϑ were unchanged. A short-term increase in the extracellular glucose concentration did not change cell surface recruitment of glucose transporter 1 or glucose transporter 4, as assessed by exofacial photolabelling with [3H]-ATB-BMPA bis-mannose. Conclusion/interpretation. Protein kinase Cβ 2 is involved in a glucose-sensitive, Ca2+-dependent signalling pathway, which is possibly involved in the regulation of glucose transport in skeletal muscle. This glucose-dependent increase in 3-0-methylglucose transport is independent of glucose transporter 4 and glucose transporter 1 translocation to the plasma membrane and may involve modifications of cell surface glucose transporter activity. [Diabetologia (1999) 42: 1071–1079]

Keywords Protein kinase C Phosphatidylinositol 3 kinase Phospholipase C GLUT 4 skeletal muscle hyperglycaemia. 

Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • Y. Kawano
    • 1
  • J. Rincon
    • 1
  • A. Soler
    • 1
  • J. W. Ryder
    • 1
  • L. A. Nolte
    • 1
  • J. R. Zierath
    • 1
  • H. Wallberg-Henriksson
    • 1
  1. 1.Departments of Clinical Physiology, and Physiology and Pharmacology, Karolinska Institute, Karolinska Hospital, Stockholm, SwedenSE

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