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Diabetologia

, Volume 42, Issue 8, pp 995–998 | Cite as

Oxidative DNA damage in diabetes mellitus: its association with diabetic complications

  • Y. Hinokio
  • S. Suzuki
  • M. Hirai
  • M. Chiba
  • A. Hirai
  • T. Toyota
Short communication

Abstract

Aims/hypothesis. Augmented oxidative stress induced by hyperglycaemia possibly contributes to the pathogenesis of diabetic complications. Oxidative stress is known to increase the conversion of deoxyguanosine to 8-oxo, 2 ′-deoxyguanosine in DNA. To investigate the possible contribution of oxidative DNA damage to the pathogenesis of diabetic complications, we measured the content of 8-oxo, 2 ′-deoxyguanosine in the urine and the blood mononuclear cells of Type II (non-insulin-dependent) diabetic patients. Methods. We studied 53 Type II diabetic patients and 39 age-matched healthy control subjects. We assayed 8-oxo, 2 ′-deoxyguanosine by HPLC-electrochemical detection method. Results. The content of 8-oxo, 2 ′-deoxyguanosine in the urine and the mononuclear cells of the Type II diabetic patients was much higher than that of the control subjects. Urinary 8-oxo, 2 ′-deoxyguanosine excretion and the 8-oxo, 2 ′-deoxyguanosine content in the mononuclear cells from the diabetic patients with complications were higher than those from the diabetic patients without complications. Urinary excretion of 8-oxo, 2 ′-deoxyguanosine was significantly correlated with the 8-oxo, 2 ′-deoxyguanosine content in the mononuclear cells. The 8-oxo, 2 ′-deoxyguanosine content in the urine and mononuclear cells was correlated with the haemoglobin A1 c value. Conclusion/interpretation. This is the first report of a direct association between oxidative DNA damage and the complications of diabetes. The augmented oxidative DNA damage in diabetes is speculated to contribute to the pathogenesis of diabetic complications. [Diabetologia (1999) 42: 995–998]

Keywords Oxidative stress 8-oxo-2 ′-deoxyguanosine diabetic complication smoking. 

Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • Y. Hinokio
    • 1
  • S. Suzuki
    • 1
  • M. Hirai
    • 1
  • M. Chiba
    • 1
  • A. Hirai
    • 1
  • T. Toyota
    • 1
  1. 1.The Third Department of Internal Medicine, Tohoku University School of Medicine, Sendai, JapanJP

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